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The map label for this gene is rnhA

Identifier: 160875176

GI number: 160875176

Start: 2452206

End: 2452676

Strand: Reverse

Name: rnhA

Synonym: Sbal195_2062

Alternate gene names: 160875176

Gene position: 2452676-2452206 (Counterclockwise)

Preceding gene: 160875181

Following gene: 160875173

Centisome position: 45.87

GC content: 49.04

Gene sequence:

>471_bases
ATGACTGAACTAAAACTGATCCACATCTTCACCGACGGCTCTTGCTTAGGCAATCCCGGTCCTGGCGGCTATGGCATTGT
AATGAATTATAAAGGCCATACAAAGGAGATGTCCGACGGTTTTGCGCTCACGACCAATAATCGCATGGAATTATTAGCCC
CGATCATAGCGTTAGAAAGCTTAAAAGAGCCTTGCCAAGTCGTGCTCACCAGCGACAGCCAATATATGCGCCAAGGCATA
ATGACGTGGATCCATGGCTGGAAGAAAAAAGGTTGGATGACATCAAACCGTACCCCAGTCAAAAACGTTGACCTGTGGAA
ACGTTTAGATAAAGCCTCACAGATGCACACTATCGACTGGCAATGGGTTAAAGGCCACGCGGGCCACGCCGAAAACGAAC
GTTGCGATGTATTAGCTCGCACCGCGGCAGAATCAAAACCGACCCAACCCGACTTAGGCTACCAGCCTTAA

Upstream 100 bases:

>100_bases
CCTGACGGCTAGCGTTAGAGATAATATTCATTTTTTATGCGCTTCTTATGACAATTTTTGGTTATACTCGGCCGAAATTC
CCGGCTATGGTAACAATTTG

Downstream 100 bases:

>100_bases
CTCTGTGGCCCTTACTTAAACTAGCTAAGTTTGAACAGCCAAAGTTCATACAAGCTAATTCGAAAAAGTAAGTTCGATGC
CATCACGCCTAAGCTAAACA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCQVVLTSDSQYMRQGI
MTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKASQMHTIDWQWVKGHAGHAENERCDVLARTAAESKPTQPDLGYQP

Sequences:

>Translated_156_residues
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCQVVLTSDSQYMRQGI
MTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKASQMHTIDWQWVKGHAGHAENERCDVLARTAAESKPTQPDLGYQP
>Mature_155_residues
TELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCQVVLTSDSQYMRQGIM
TWIHGWKKKGWMTSNRTPVKNVDLWKRLDKASQMHTIDWQWVKGHAGHAENERCDVLARTAAESKPTQPDLGYQP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=34.0277777777778, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=65.7894736842105, Blast_Score=220, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=36.1702127659575, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SHEB2 (B8E599)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358246.1
- ProteinModelPortal:   B8E599
- SMR:   B8E599
- GeneID:   7087738
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2324
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17584; Mature: 17453

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALES
CCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCQVVLTSDSQYMRQGIMTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKASQMHTIDW
HCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCE
QWVKGHAGHAENERCDVLARTAAESKPTQPDLGYQP
EEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALES
CCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCQVVLTSDSQYMRQGIMTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKASQMHTIDW
HCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCE
QWVKGHAGHAENERCDVLARTAAESKPTQPDLGYQP
EEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA