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The map label for this gene is gloB [H]

Identifier: 160875173

GI number: 160875173

Start: 2449381

End: 2450172

Strand: Reverse

Name: gloB [H]

Synonym: Sbal195_2059

Alternate gene names: 160875173

Gene position: 2450172-2449381 (Counterclockwise)

Preceding gene: 160875176

Following gene: 160875172

Centisome position: 45.82

GC content: 49.37

Gene sequence:

>792_bases
ATGCTCACTATAACAGCGATCAAAGCCTTTAATGACAATTATATCTGGGTGTTACAACAACAGCCCCATACACAGGTTTA
TGTCGTCGATCCCGGCGATGCGAGTGTGGTGATTGACTATCTAGAGGCCAATCAACTCACGCTAGTGGGCATTTTACTGA
CCCACCACCACAATGACCATACGGGTGGCGTCGCTGAACTTCAAGCCTACAGTCAAGACCGACTCACAGTGTATGGCCCA
GATAATGAGAAGATTGAAGGCATCACCCATCCGTTGCACGCAACAGCACAGCCCAGATTTACACTCGACTATATCAGTGG
CGAACTGCAAGTCTTGGATGTACCGGGGCACACAGCAGGACATATCGCCTATGTTATTGCAGATGCCTTATTCTGCGGTG
ATACCTTATTTAGCGGTGGCTGCGGACGCTTATTTGAAGGTACGCCAGCGCAAATGCTCAACTCACTACAGCAACTTGCC
CAGCTTCCCGCCTACACTCGGGTGTATTGTGCCCATGAGTACACACTTTCAAACCTCAAATTTGCCCTCGCGGTCAACCC
CAACAATTGCGCGCTGCAAGACTATAACGAACGCGCCATAGCGTTGCGTCGCCAAGACAAAGCGACTATCCCCTCCACCA
TCGCCTTAGAACGCGCCATCAATCCCTTTCTACGTGCCAGCGACACTGAGATTGTTGGCAGCATAAAACAGCATTTTAGC
GATCTAAGTCATGCCAATTTAGACGAACTTGGCGGTTTTACCCTGCTCAGACAGTGGAAAGATAATTTTTGA

Upstream 100 bases:

>100_bases
CCATTTAGCCATAGGGCAAAAGCGGCAAAGGTCGCCAAGGGCCGTTCACTCATGGATAATACCTTTATCTTCGAATTGAA
ATACAGACATAGGCGGTTCT

Downstream 100 bases:

>100_bases
TCACAAAAGCAATATTGTAGATTGAAATTTAAACAGTTTTTTCCATTTTTGATGATGTAAATCAAAAATGGCCTTACATT
ATGTACGATAATTAAGGGAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLVGILLTHHHNDHTGGVAELQAYSQDRLTVYGP
DNEKIEGITHPLHATAQPRFTLDYISGELQVLDVPGHTAGHIAYVIADALFCGDTLFSGGCGRLFEGTPAQMLNSLQQLA
QLPAYTRVYCAHEYTLSNLKFALAVNPNNCALQDYNERAIALRRQDKATIPSTIALERAINPFLRASDTEIVGSIKQHFS
DLSHANLDELGGFTLLRQWKDNF

Sequences:

>Translated_263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLVGILLTHHHNDHTGGVAELQAYSQDRLTVYGP
DNEKIEGITHPLHATAQPRFTLDYISGELQVLDVPGHTAGHIAYVIADALFCGDTLFSGGCGRLFEGTPAQMLNSLQQLA
QLPAYTRVYCAHEYTLSNLKFALAVNPNNCALQDYNERAIALRRQDKATIPSTIALERAINPFLRASDTEIVGSIKQHFS
DLSHANLDELGGFTLLRQWKDNF
>Mature_263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLVGILLTHHHNDHTGGVAELQAYSQDRLTVYGP
DNEKIEGITHPLHATAQPRFTLDYISGELQVLDVPGHTAGHIAYVIADALFCGDTLFSGGCGRLFEGTPAQMLNSLQQLA
QLPAYTRVYCAHEYTLSNLKFALAVNPNNCALQDYNERAIALRRQDKATIPSTIALERAINPFLRASDTEIVGSIKQHFS
DLSHANLDELGGFTLLRQWKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=270, Percent_Identity=34.4444444444444, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=34.4444444444444, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=35.0210970464135, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI116642887, Length=234, Percent_Identity=37.6068376068376, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI21703352, Length=234, Percent_Identity=37.6068376068376, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=35.8974358974359, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1786406, Length=238, Percent_Identity=45.3781512605042, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787158, Length=214, Percent_Identity=30.8411214953271, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=274, Percent_Identity=29.9270072992701, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320478, Length=236, Percent_Identity=29.6610169491525, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=241, Percent_Identity=30.7053941908714, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=270, Percent_Identity=36.6666666666667, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24667711, Length=270, Percent_Identity=36.6666666666667, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24667703, Length=270, Percent_Identity=36.6666666666667, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29145; Mature: 29145

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLVGILLTHHHNDH
CEEEEEEEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEEEECCCCC
TGGVAELQAYSQDRLTVYGPDNEKIEGITHPLHATAQPRFTLDYISGELQVLDVPGHTAG
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCHH
HIAYVIADALFCGDTLFSGGCGRLFEGTPAQMLNSLQQLAQLPAYTRVYCAHEYTLSNLK
HHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCEE
FALAVNPNNCALQDYNERAIALRRQDKATIPSTIALERAINPFLRASDTEIVGSIKQHFS
EEEEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
DLSHANLDELGGFTLLRQWKDNF
HCCCCCHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLVGILLTHHHNDH
CEEEEEEEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEEEECCCCC
TGGVAELQAYSQDRLTVYGPDNEKIEGITHPLHATAQPRFTLDYISGELQVLDVPGHTAG
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCHH
HIAYVIADALFCGDTLFSGGCGRLFEGTPAQMLNSLQQLAQLPAYTRVYCAHEYTLSNLK
HHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCEE
FALAVNPNNCALQDYNERAIALRRQDKATIPSTIALERAINPFLRASDTEIVGSIKQHFS
EEEEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
DLSHANLDELGGFTLLRQWKDNF
HCCCCCHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA