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The map label for this gene is iadA [H]

Identifier: 160875161

GI number: 160875161

Start: 2431531

End: 2432700

Strand: Reverse

Name: iadA [H]

Synonym: Sbal195_2047

Alternate gene names: 160875161

Gene position: 2432700-2431531 (Counterclockwise)

Preceding gene: 160875162

Following gene: 160875160

Centisome position: 45.49

GC content: 47.86

Gene sequence:

>1170_bases
ATGTTAACTTTAATCCGTAACGCCGCCGTTTATGCGCCGCAGTTTTTAGGGCTACGCGACGTGTTATTTTGCGAGGCGAA
AGTCCTTAAAATCGCCCCCGTCATTGATCTTAAGCATGCAGACTTAGTCACAATCATCGAAGCCAATGGCCGCCCTTTAA
TCCCAGGTTTAGTGGACCCCTTAGTGCATATCACAGGCGGTGGCGGTGAAGGCGGTTACCACACGCGTACTCCAGAGATG
CAACTAACAGATGCTAGCCTTGCTGGGGTCACTACGCTCGTCGCAGCATTGGGAACCGACGCCATTAGTCGCAGCTTGAA
CGAACTTGTCGTCAAAGCGAAAGCGCTCACATACGAAGGCTTGTCTGTCTTTTGCTACACAGGCTCTTACGAGTACCCCG
TAAAGACCCTTACTGGCGATATTACCCAAGATCTGGTGTTAATCGACAATGTTATTGGTGTGGGTGAGATTGCAATTGCA
GATCACCGAGGATCGCAACTCACTGTGCCAGAACTCGCCAGAGTCGCAGCGCAAAGCCGAGTCGGTGGCATGCTCAGCGG
TAAAGGCGGCATAGTGTTTGTGCATGTGGGCGATGGTGCAACTGGTATCCAAATTTTGCATCACATCGCGCAGCAGTCTG
ATATCCCTTTAACGCAGTTCTATCCCACTCACATGAATCGCTCCCAACAGCTATTAGAGCAAGGGCTTGAATTTGCACGA
CAAGGTGGGGTTATCGATTTCACGACGTCGACTAATACTCAATATTTAAACGAGGGTGAAATCGCGGTCGTTGATGCGGT
CCATTTTGCAATCTCGCAAGGTATCAACTTGAGTCAAATCAGCTTAAGTTCCGACGGTAATGCTAGCCTGCCTGTATTTA
ATGCTGACGGAGAGTGTATTGGAATAGAAATGGGTAAAGTCAGCAGTCTATTTGAAGCGCTAGTGGCTTTAATTAACAGT
GGTTTATCCCTAGAACAAGTGCTTCCTTTAGTGACCTCAAATGCCGCTCGGGTGCTCAAGCTAAAAAGTAAGGGCGGGAT
AAGCGAAACACACGATGCCGACTTTGTGTTATTAACGGATAACTTTAATATCGATAAAGTATGGAGCAAAGGGCAACTAC
TCGTTGATGAAGGTAACGCACTGAAATGGGGAACATTTGAATCGCAGTAA

Upstream 100 bases:

>100_bases
CATACGCTCAATGGCTTGCGCTTATTTGGCGCTTTCAATTATTGCTGACCTTGATGGCCAGTGCCGTCATTTTATTTGCC
GTTATTACAGGATATAGCTG

Downstream 100 bases:

>100_bases
CTCAAAATGGCGCACGCGGCGCGCGTGCACCACACTCATAAACTGTGTGAAATGCGAGTAGATTACTCGCAAACAGAGCG
TATCAAGCTCACTTTCTCAA

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDITQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGATGIQILHHIAQQSDIPLTQFYPTHMNRSQQLLEQGLEFAR
QGGVIDFTTSTNTQYLNEGEIAVVDAVHFAISQGINLSQISLSSDGNASLPVFNADGECIGIEMGKVSSLFEALVALINS
GLSLEQVLPLVTSNAARVLKLKSKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESQ

Sequences:

>Translated_389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDITQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGATGIQILHHIAQQSDIPLTQFYPTHMNRSQQLLEQGLEFAR
QGGVIDFTTSTNTQYLNEGEIAVVDAVHFAISQGINLSQISLSSDGNASLPVFNADGECIGIEMGKVSSLFEALVALINS
GLSLEQVLPLVTSNAARVLKLKSKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESQ
>Mature_389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDITQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGATGIQILHHIAQQSDIPLTQFYPTHMNRSQQLLEQGLEFAR
QGGVIDFTTSTNTQYLNEGEIAVVDAVHFAISQGINLSQISLSSDGNASLPVFNADGECIGIEMGKVSSLFEALVALINS
GLSLEQVLPLVTSNAARVLKLKSKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESQ

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=388, Percent_Identity=39.6907216494845, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 41360; Mature: 41360

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDP
CEEEEECCHHHCHHHCCHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCEECCHHHCC
LVHITGGGGEGGYHTRTPEMQLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEG
EEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
LSVFCYTGSYEYPVKTLTGDITQDLVLIDNVIGVGEIAIADHRGSQLTVPELARVAAQSR
CEEEEEECCCCCCHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHHH
VGGMLSGKGGIVFVHVGDGATGIQILHHIAQQSDIPLTQFYPTHMNRSQQLLEQGLEFAR
CCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHEECCCCCCHHHHHHHHHHHHHH
QGGVIDFTTSTNTQYLNEGEIAVVDAVHFAISQGINLSQISLSSDGNASLPVFNADGECI
CCCEEEEECCCCCEEECCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEE
GIEMGKVSSLFEALVALINSGLSLEQVLPLVTSNAARVLKLKSKGGISETHDADFVLLTD
EEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEC
NFNIDKVWSKGQLLVDEGNALKWGTFESQ
CCCHHEECCCCEEEEECCCEEECCCCCCC
>Mature Secondary Structure
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDP
CEEEEECCHHHCHHHCCHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCEECCHHHCC
LVHITGGGGEGGYHTRTPEMQLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEG
EEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
LSVFCYTGSYEYPVKTLTGDITQDLVLIDNVIGVGEIAIADHRGSQLTVPELARVAAQSR
CEEEEEECCCCCCHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHHH
VGGMLSGKGGIVFVHVGDGATGIQILHHIAQQSDIPLTQFYPTHMNRSQQLLEQGLEFAR
CCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHEECCCCCCHHHHHHHHHHHHHH
QGGVIDFTTSTNTQYLNEGEIAVVDAVHFAISQGINLSQISLSSDGNASLPVFNADGECI
CCCEEEEECCCCCEEECCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEE
GIEMGKVSSLFEALVALINSGLSLEQVLPLVTSNAARVLKLKSKGGISETHDADFVLLTD
EEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEC
NFNIDKVWSKGQLLVDEGNALKWGTFESQ
CCCHHEECCCCEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]