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The map label for this gene is prtR [H]

Identifier: 160875134

GI number: 160875134

Start: 2397352

End: 2398053

Strand: Reverse

Name: prtR [H]

Synonym: Sbal195_2020

Alternate gene names: 160875134

Gene position: 2398053-2397352 (Counterclockwise)

Preceding gene: 160875147

Following gene: 160875126

Centisome position: 44.85

GC content: 45.87

Gene sequence:

>702_bases
ATGAAAACACTCGGTGAACGCTCTAAAGAGCGAAGAAAAACTATCAACCTGACTCAACTCCAACTTAGTAAAAAAGTAGG
AGTTTCAAGCGTTACCATTTCACAATGGGAATCAGGTGACACATCTCCAAAGGGTGAAAATCTTTACAAGTTGGCTCAAG
CACTTCAGTGCTCACCTGACTGGTTGATGTTTGGCGATAGCAAAAACACGAAGAAACCTGAATCGAATGCTGAATGGGCA
GGCGGTTTTGATCTATGGGACAACGATAGCCCACTGGGTGAGGATGAAGTTGAAATCCCCTTTTACGTGGATATCGAACT
CTCAGCGGGTGAAGGCATAGTTGAAGGTGTTGAATATAAAGGCCCTAAGCTCCGTTTTGCCAAAAGCAGCCTTAAGCGTC
AAGGCGTAAACACAGACCATGCGGCCTGTGTAAAAGTGAATGGCAACAGCATGGAACCTGTATTACCCCACGGCTCAACT
GTGGGGGTAGATACCTCAGCCACCGAAATTGTTGATGGTAAAATGTACGCCATTAACCACGATGGCATGATGCGGGTCAA
AATGCTCTACAAACTTCCCGGCGGTGGCCTGCGCCTACGTAGCTACAACACGGATGAGTGGCCAGACGAGCACATCAATA
ACGAACAACTTAAAAACATTAAAGTCATCGGCAAAGTGTTTTGGTACTCAGTTCTGTTGTAA

Upstream 100 bases:

>100_bases
GACGGCAAGCTGAGTTTTCATTTTCCACCACCCTAAACTTAAGTTTTCCTAAGTATCAATACAAATAAAACCTAAGTCAA
ATTGATTTAAGATAACTTAT

Downstream 100 bases:

>100_bases
AGCATGTTCAATTCGAAAATAGCTTTTCCAACATATTGTTGAGTATAACCCTGTTAAACAATTACGCTTATTAGCCAGAT
TCAGCTTAACTATTCTATTA

Product: putative phage repressor

Products: NA

Alternate protein names: Pyocin repressor protein [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKTLGERSKERRKTINLTQLQLSKKVGVSSVTISQWESGDTSPKGENLYKLAQALQCSPDWLMFGDSKNTKKPESNAEWA
GGFDLWDNDSPLGEDEVEIPFYVDIELSAGEGIVEGVEYKGPKLRFAKSSLKRQGVNTDHAACVKVNGNSMEPVLPHGST
VGVDTSATEIVDGKMYAINHDGMMRVKMLYKLPGGGLRLRSYNTDEWPDEHINNEQLKNIKVIGKVFWYSVLL

Sequences:

>Translated_233_residues
MKTLGERSKERRKTINLTQLQLSKKVGVSSVTISQWESGDTSPKGENLYKLAQALQCSPDWLMFGDSKNTKKPESNAEWA
GGFDLWDNDSPLGEDEVEIPFYVDIELSAGEGIVEGVEYKGPKLRFAKSSLKRQGVNTDHAACVKVNGNSMEPVLPHGST
VGVDTSATEIVDGKMYAINHDGMMRVKMLYKLPGGGLRLRSYNTDEWPDEHINNEQLKNIKVIGKVFWYSVLL
>Mature_233_residues
MKTLGERSKERRKTINLTQLQLSKKVGVSSVTISQWESGDTSPKGENLYKLAQALQCSPDWLMFGDSKNTKKPESNAEWA
GGFDLWDNDSPLGEDEVEIPFYVDIELSAGEGIVEGVEYKGPKLRFAKSSLKRQGVNTDHAACVKVNGNSMEPVLPHGST
VGVDTSATEIVDGKMYAINHDGMMRVKMLYKLPGGGLRLRSYNTDEWPDEHINNEQLKNIKVIGKVFWYSVLL

Specific function: Represses the promoter activity of the prtN gene [H]

COG id: COG2932

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787853, Length=71, Percent_Identity=47.887323943662, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF01381 HTH_3; PF00717 Peptidase_S24 [H]

EC number: NA

Molecular weight: Translated: 25940; Mature: 25940

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLGERSKERRKTINLTQLQLSKKVGVSSVTISQWESGDTSPKGENLYKLAQALQCSPD
CCCHHHHHHHHHHHCCHHHEEHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
WLMFGDSKNTKKPESNAEWAGGFDLWDNDSPLGEDEVEIPFYVDIELSAGEGIVEGVEYK
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCC
GPKLRFAKSSLKRQGVNTDHAACVKVNGNSMEPVLPHGSTVGVDTSATEIVDGKMYAINH
CCEEEHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCEEECCEEEEECC
DGMMRVKMLYKLPGGGLRLRSYNTDEWPDEHINNEQLKNIKVIGKVFWYSVLL
CCEEEEEEEEECCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLGERSKERRKTINLTQLQLSKKVGVSSVTISQWESGDTSPKGENLYKLAQALQCSPD
CCCHHHHHHHHHHHCCHHHEEHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
WLMFGDSKNTKKPESNAEWAGGFDLWDNDSPLGEDEVEIPFYVDIELSAGEGIVEGVEYK
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCC
GPKLRFAKSSLKRQGVNTDHAACVKVNGNSMEPVLPHGSTVGVDTSATEIVDGKMYAINH
CCEEEHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCEEECCEEEEECC
DGMMRVKMLYKLPGGGLRLRSYNTDEWPDEHINNEQLKNIKVIGKVFWYSVLL
CCEEEEEEEEECCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444788; 10984043 [H]