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The map label for this gene is napA [H]

Identifier: 160875058

GI number: 160875058

Start: 2322491

End: 2324980

Strand: Direct

Name: napA [H]

Synonym: Sbal195_1943

Alternate gene names: 160875058

Gene position: 2322491-2324980 (Clockwise)

Preceding gene: 160875057

Following gene: 160875059

Centisome position: 43.43

GC content: 50.44

Gene sequence:

>2490_bases
ATGAACAGACGTGATTTTATTAAGGCCAATGCCGTGATTGCCGCTGCAGGGGCCGCAGGTTTAGCCTTGCCCGCTGGCGC
AAGTAACCTGATAACCAGCTCAGAACAAACCAAATTAGAGTGGAACAAGGCTCCATGCCGTTTCTGTGGTACGGGCTGCT
CTGTGATTGTCGCCACTCGCGACGGCAAAGTGGTGGCCACCCATGGCGATGCCAACAGTGAAGTGAACCGTGGACTTAAC
TGTATCAAGGGTTATTTCCTATCGAAAATCATGTATGGCAGCGATCGCTTAACCACGCCCATGCTGCGAATGACCGATGG
CAAATACGACAAGCACGGCGAGTTTTCGCCGATAAGCTGGGACAGCGCCTTCGATATCATGGCGCAAAAATGGAAAGAGA
CCATTAAAGCCAAGGGCCCAACCGCTGTGGGCATGTTTGGTTCGGGCCAATGGACAGTGTGGGAAGGTTACGCCGCCGTT
AAGTTGATGAAGGCGGGCTTTGGCTCTAACAATATCGACCCTAATGCGCGTCATTGTATGGCGTCGGCCGTGGTCGGTTT
TATGCGCACTTTCGGTATCGATGAGCCAATGGGCTGCTATGACGATATGGAAGCCGCCGATGCGTTTGTGCTTTGGGGCT
CGAACATGGCCGAAATGCACCCGATCCTGTGGAGCCGCGTGACCGACAGACGCTTGAGTGAACCTCATGTCAAAGTGGCC
GTGTTATCGACTTTCCAACATCGCTCCTTCGATCTGGCAGATTTACCGATAGTGTTCACTCCGCAAACCGATTTGGCCAT
GTTGAACTTTATTGCTAACTATATTATTCAAAACGATAAAGTGAACTGGGATTTCGTGAAGAAACACGTAAATTTCCGCC
AAGGCACGACTGATATCGGCTACGGTTTACGTCCGACTCATCCGCTTGAACAGAAAGCCAAAAACGTCAAAACGGCGGGG
GACTCAACGCCAATCGATTTTGAAGCCTTCAAAGCCTTTGTTGCCGAATACACAGTCGAGAAAGTCAGTAAACTGTCTGG
CGTGCCAGAAGATAAACTGATCGAACTGGCGAAGCTGTATGCTGATCCTAATACGAAAGTGACGTCATTCTGGACCATGG
GCTTTAACCAACATACCCGTGGTGTGTGGTGTAACAACCTGATTTACAACATCCATTTATTAACAGGTAAGATTTCTACC
CCTGGCAACAGTCCGTTCTCGCTGACAGGTCAGCCATCGGCCTGTGGCACGGCCCGTGAAGTCGGGACTTTCTCTCACCG
TTTACCTGCGGACATGGTGGTGACCAATCCCGAGCACAGAAAACACGCTGAAGAAACGTGGAAAATTCCAAGCGGCATTA
TTCCACCTAAACCCGGTTATCATGCTGTCGAGCAAAATCGCCGCCTAAAAGACGGTGACTTGAACTGCTATTGGGTGCAA
GTGAACAACAATATGCAGGCGGGTCCGAATATCAACGAAGAAGGGTTGCCGGGCTATCGTAATCCCGAAAACTTTATCGT
GGTGTCCGATGCTTATCCCACCGTCACCACTCAAGCTGCCGATCTTATTTTGCCCACTGCAATGTGGGTCGAGAAAGAGG
GCGCTTACGGTAACGCCGAGCGCCGTACTCAGTTCTGGCATCAAATGGTGCAAGCGCCGGGCGAATCGCATTCGGATCTG
TGGCAGTTGATGGAATTTGCGAAACGTTTCACCACAGATGAAGTGTGGCCCAAGGATGTATTAGCGGTGAACCCTGAGTT
CAAAGGCAAAACGCTTTTCGAAGTCCTGTACCAAAACGGCAATGTTGATAAGTTCCCATTGACGGATGCGGATCCTAAGT
ATCTGAACGATGAAGCTAAACATTTTGGTTTCTATGTGCAAAAAGGCCTGTTTGAAGAATACGCCTCCTTTGGACGCGGT
CATGGCCACGACTTAGCACCATTTGATGCTTATCACGAGGCCCATGGTCTGCGTTGGCCTGTGGTCGATGGCAAGGAAAC
CAAGTGGCGTTTCCGTGAGGGGAGCGATCCTTATGTTAAAGCAGGTAAGGGCTTTGAGTTTTATGGTCATCCCGATGGCC
GTGCGGTGATTTTTGCGCTGCCCTATGAGCCCGCCGCCGAGTCGCCAGACGAAGAATACGATATCTGGTTATCGACGGGC
CGAGTGCTGGAACATTGGCATTCGGGTTCTATGACCCAACGTGTGCCTGAGCTTTATCGTGCTTTCCCTGATGCTGTGTG
TTTTATGCATCCCGAAGATGCCAAGAAACGCGGTCTGCGCCGCGGTGACGAAATTCGTGTGGTGTCACGACGGGGTGAAA
TTAAGACCCGAGTTGAAACCCGTGGCCGTAATAAACCGCCTGTGGGCTTAGTGTTTGTGCCTTGGTTTGATGCTAGCCAG
CTCATTAATAAAGTCACTTTAGATGCGACAGATCCCTTGTCAAAACAAACTGACTTTAAAAAATGTGCGGTGAAGATCGT
CAAGGTTTAA

Upstream 100 bases:

>100_bases
TGATGGACTGCATAGAAGCGATCAATGCCATTCCGGGTGTGTTATCCGCCACTATGGTTTACCACCAGAGTGAAACGCTC
GAAGAGGGTGAAGAATGAAT

Downstream 100 bases:

>100_bases
GGAGAACACGATGAAAACTCTACAATTAGTTTCAGCGATCGGTTTACTGGCATTCGGTTCATTGATGATGACGGCAGTCT
CTGCCGCCAGTATTCCCGAC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQHRSFDLADLPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNTKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAVNPEFKGKTLFEVLYQNGNVDKFPLTDADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGKGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Sequences:

>Translated_829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQHRSFDLADLPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNTKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAVNPEFKGKTLFEVLYQNGNVDKFPLTDADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGKGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV
>Mature_829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQHRSFDLADLPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNTKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAVNPEFKGKTLFEVLYQNGNVDKFPLTDADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGKGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=829, Percent_Identity=72.0144752714113, Blast_Score=1281, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=23.5813366960908, Blast_Score=212, Evalue=8e-56,
Organism=Escherichia coli, GI3868720, Length=666, Percent_Identity=23.1231231231231, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787870, Length=885, Percent_Identity=23.0508474576271, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI3868719, Length=480, Percent_Identity=23.3333333333333, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI87081797, Length=895, Percent_Identity=22.4581005586592, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92966; Mature: 92966

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
VLSTFQHRSFDLADLPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTPIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNTKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKHAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFAKRFTTDEVWPKDVLAVNPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCC
NVDKFPLTDADPKYLNDEAKHFGFYVQKGLFEEYASFGRGHGHDLAPFDAYHEAHGLRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKAGKGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC
>Mature Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
VLSTFQHRSFDLADLPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTPIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNTKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKHAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFAKRFTTDEVWPKDVLAVNPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCC
NVDKFPLTDADPKYLNDEAKHFGFYVQKGLFEEYASFGRGHGHDLAPFDAYHEAHGLRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKAGKGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA