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The map label for this gene is iaaA [H]

Identifier: 160875052

GI number: 160875052

Start: 2314313

End: 2315293

Strand: Direct

Name: iaaA [H]

Synonym: Sbal195_1937

Alternate gene names: 160875052

Gene position: 2314313-2315293 (Clockwise)

Preceding gene: 160875049

Following gene: 160875053

Centisome position: 43.28

GC content: 50.56

Gene sequence:

>981_bases
ATGTCACACAACACCTTTGCCAATGACAAACCGTTCGCGATTGCGATCCACGGCGGCGCTGGCACGATTTCTAAGGCTAA
TCTCACCCCTGAACAACGCCAAGCCTACAAAGATAAGTTAAAGGAAGCCGTTGATAAAGGCTCAAAAGTGTTAGAAGAGG
GCGGTGACAGTTTAGTCGCGGTGCAAACAGCCATTAATATTCTCGAAAATAGCCCACTGTTCAATGCTGGCATAGGCGCG
GTGTACACCTATGACGGCGGGCATGAGTTAGATGCGTCCATTATGGATGGCAAAACCATGAATGCTGGCGCAATTGCTGG
AGTGCGCCATATCGCCAATCCCATTGATCTCGCTTTAGCCGTGATGGATAAGTCTGAGCATGTGATGCTCTCGGGCGCAG
GTGCAGAAGAATTTGCCCTGACTCAAGGCTTTAGCTTAGTGCCAAACAGTCATTTTGATACTGACGAGCGTTATCAGCAA
TTACTCGATGCTCGCCAAAAACTGCAAGCTGCGGCTAAATCAGAGCAAGTCGCGGGTGTTGAGATGAAAGATCTCGATTA
CAAATTTGGTACCGTAGGCGCCGTCGCCTTAGACAAAAATGGCAACTTAGCTGCTGGTACCTCAACCGGCGGCATGACGG
CAAAACGCTTCGGCCGCATTGGAGATTCCCCTGTGATTGGTGCCGGTACTTATGCTGAAAATGGGGTGTGCGCCGTATCG
GCAACCGGCCATGGTGAGTTCTTTATTCGTTATCAAGTGGCGGGGGATATTTGTGCCAAAGTAAAATACCAGCAGAAATC
CATTATTCAAGCCGCCGATGAAGTGATCAATCAACGTCTTATCACCGCTGGTGGTTCGGGCGGCGTGATTGCCGTCGATC
AGCGCGGTAATATCGCCACGCCATTTAACACTGAAGGCATGTACCGCGCTACCCGTAGCAATGGCGAACCCGCCCAAGTG
CTGATCTGGCAAGACCAATAA

Upstream 100 bases:

>100_bases
CACGATGAAATGGGTACAAAGGACGCTACTTTTTAAGGGGATGTAAAGAATGAAAACAAAATTAGCCACCTTTAGTTTAA
TTATCTCGGGTTTACTTTTT

Downstream 100 bases:

>100_bases
CTTAAATTGTAAAAGCAGTTGGTTCGAGACGGACATTGCTTACAAGATTGTCTAGAAACATCACTTATAAACCTTAAAAA
GCGAGTGCTGCGAAAGTGGC

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQTAINILENSPLFNAGIGA
VYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMDKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQ
LLDARQKLQAAAKSEQVAGVEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVS
ATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFNTEGMYRATRSNGEPAQV
LIWQDQ

Sequences:

>Translated_326_residues
MSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQTAINILENSPLFNAGIGA
VYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMDKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQ
LLDARQKLQAAAKSEQVAGVEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVS
ATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFNTEGMYRATRSNGEPAQV
LIWQDQ
>Mature_325_residues
SHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQTAINILENSPLFNAGIGAV
YTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMDKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQL
LDARQKLQAAAKSEQVAGVEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVSA
TGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFNTEGMYRATRSNGEPAQVL
IWQDQ

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=306, Percent_Identity=38.562091503268, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI145275200, Length=306, Percent_Identity=38.562091503268, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI98991769, Length=323, Percent_Identity=29.4117647058824, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI285002251, Length=231, Percent_Identity=32.9004329004329, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=50.7987220447284, Blast_Score=294, Evalue=7e-81,
Organism=Drosophila melanogaster, GI18921183, Length=305, Percent_Identity=37.0491803278689, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI21357893, Length=271, Percent_Identity=33.5793357933579, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI221330102, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652135, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 34355; Mature: 34224

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVA
CCCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VQTAINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALA
HHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCHHHHHHHHHCCHHHEEEE
VMDKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQLLDARQKLQAAAKSEQVAGV
EECCCCEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCC
EMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVS
CHHCCCEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCCCCCEEEEE
ATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIAT
ECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCC
PFNTEGMYRATRSNGEPAQVLIWQDQ
CCCCCCCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
SHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVA
CCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VQTAINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALA
HHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCHHHHHHHHHCCHHHEEEE
VMDKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQLLDARQKLQAAAKSEQVAGV
EECCCCEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCC
EMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVS
CHHCCCEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCCCCCEEEEE
ATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIAT
ECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCC
PFNTEGMYRATRSNGEPAQVLIWQDQ
CCCCCCCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]