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The map label for this gene is hydA [H]

Identifier: 160875037

GI number: 160875037

Start: 2293416

End: 2294552

Strand: Reverse

Name: hydA [H]

Synonym: Sbal195_1922

Alternate gene names: 160875037

Gene position: 2294552-2293416 (Counterclockwise)

Preceding gene: 160875048

Following gene: 160875036

Centisome position: 42.91

GC content: 51.54

Gene sequence:

>1137_bases
ATGGACACACATGCAGCCCTATATGAACAGGGAAAGGCGCGCTTAGATGCGTTACGCCAATTCGCTCCCCGTCAACAACA
ATCGCTGATTGAGAAAATGCAACAGCACGGCATTAGCCGCCGTGATTTTATGAAGTGGAGTGCCATGGTGACGAGCATGC
TCGCATTGCCGCTCCCCTTTAGTAACTTAGTAGCCGAAGCTGCCGAATTAGCCGACCGTGTACCCTTAATCTGGTTACAC
ATGGCCGAATGTACTGGCTGCTCAGAATCCTTAATTCGTACTGATACCCCCAATCTAGATTCGCTGATTTTTGACCATAT
CTCCTTGGAATATCACGAAACCTTAATGGCTGCTGCGGGTTGGCAAGCCGAAGAGAACCTCGAAAATGCACTCGAAACCT
ACAAGGGCCGCTATTTACTGGCCGTTGAAGGGGCGATTCCAACCGCCAATAACGGCAGCTTCCTCACTGTGGGCTGTAAA
GGCCACACAGGATTAGAAATCATCAAGCATGCTGCAGAAGGCGCCGCCGCGATTATCTCTGTCGGCACTTGTGCGGCGTT
TGGTGGTGTGCAAGCCGCCTATCCAAACCCAACGGGGGCGAAAGGCGTGCACGAAGTGGTCAGTAAACCCGTCATTAACT
TAGGCGGTTGCCCACCGAGCGAAAAGAACATAGTCGGCACTTTGATGTACTTCATCATGTTCGGCAAATTACCTGCGCTG
GATATGTTTAACAGACCAAAATGGGCTTACGGCGCACGGGTACACGATAACTGTGAACGTCGCGGCCGCTTTGATGCCGG
TGAATTCGTTGAAGAATTTGGCGATCACGGCGCGAAAGAAGGCTACTGCCTGTATAAAGTCGGTTGTAAAGGTCCTTACA
CCTACAACAACTGCCCTACCGAGCGCTTTAACCATCACACCAGCTGGCCAGTACTTGCTGGACACGGTTGTATGGGCTGC
TCAGAACCTAACTTCTGGGATGACATGGCCGACTTTGAAAAACCCCTTGGCCGTCAACTCCTCCATGGATTGGATGCGAC
CGCAGATACTATTGGTGCGGTGATTTTAGGCGCGACTGTTGTGGGCATTGGAGCCCATGCCGTTGCCAGTATTTTTGCCA
AACCGCTGGAGGAATAA

Upstream 100 bases:

>100_bases
ATATACATCAAGAATCTTTACACTCCAACGGCCTAAACTGACCCTGTCAATTATGTAAACCCTTACCAATTTACATTTAA
ATAACAAAAAAGGTTGACCC

Downstream 100 bases:

>100_bases
CCCATGAGCAAGCGCGTTGTTATCGACCCTATCACACGTATCGAGGGTCACTTACGTATCGAAGTTGAAGTTGATGAAAA
TAACGTCATCAGCAAGGCTT

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDTHAALYEQGKARLDALRQFAPRQQQSLIEKMQQHGISRRDFMKWSAMVTSMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLIRTDTPNLDSLIFDHISLEYHETLMAAAGWQAEENLENALETYKGRYLLAVEGAIPTANNGSFLTVGCK
GHTGLEIIKHAAEGAAAIISVGTCAAFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASIFAKPLEE

Sequences:

>Translated_378_residues
MDTHAALYEQGKARLDALRQFAPRQQQSLIEKMQQHGISRRDFMKWSAMVTSMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLIRTDTPNLDSLIFDHISLEYHETLMAAAGWQAEENLENALETYKGRYLLAVEGAIPTANNGSFLTVGCK
GHTGLEIIKHAAEGAAAIISVGTCAAFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASIFAKPLEE
>Mature_378_residues
MDTHAALYEQGKARLDALRQFAPRQQQSLIEKMQQHGISRRDFMKWSAMVTSMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLIRTDTPNLDSLIFDHISLEYHETLMAAAGWQAEENLENALETYKGRYLLAVEGAIPTANNGSFLTVGCK
GHTGLEIIKHAAEGAAAIISVGTCAAFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASIFAKPLEE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=333, Percent_Identity=43.5435435435435, Blast_Score=283, Evalue=9e-78,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=50.3424657534247, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 41101; Mature: 41101

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHAALYEQGKARLDALRQFAPRQQQSLIEKMQQHGISRRDFMKWSAMVTSMLALPLPF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
SNLVAEAAELADRVPLIWLHMAECTGCSESLIRTDTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLENALETYKGRYLLAVEGAIPTANNGSFLTVGCKGHTGLEIIKHAAEGAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHH
VGTCAAFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASIFAKPLEE
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTHAALYEQGKARLDALRQFAPRQQQSLIEKMQQHGISRRDFMKWSAMVTSMLALPLPF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
SNLVAEAAELADRVPLIWLHMAECTGCSESLIRTDTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLENALETYKGRYLLAVEGAIPTANNGSFLTVGCKGHTGLEIIKHAAEGAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHH
VGTCAAFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASIFAKPLEE
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]