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The map label for this gene is hydB [H]

Identifier: 160875036

GI number: 160875036

Start: 2291709

End: 2293412

Strand: Reverse

Name: hydB [H]

Synonym: Sbal195_1921

Alternate gene names: 160875036

Gene position: 2293412-2291709 (Counterclockwise)

Preceding gene: 160875037

Following gene: 160875035

Centisome position: 42.89

GC content: 49.77

Gene sequence:

>1704_bases
ATGAGCAAGCGCGTTGTTATCGACCCTATCACACGTATCGAGGGTCACTTACGTATCGAAGTTGAAGTTGATGAAAATAA
CGTCATCAGCAAGGCTTGGTCATCCTCCACACTCTGGCGCGGTATTGAAGTCATCCTCAAGGGCCGTACACCTATGGATG
TTGGCCTTATTGTGCAACGTATCTGTGGTGTCTGTACCTATTCCCACTACCGCTGCGGCACTGAAGCGGTTGAGAACGCT
CTGGGAATTCAAATTCCGTTGAACGCGAGATACTTACGTTCATTGATGCAAACCTCTTTATACATGCATGACCATATTGT
GCATTTCTATCATCTACACGGTCTTGATTGGGTGGATGTGGTGTCTGCACTGAGCGCCGATCCGGCCAAAGCTGCACAAG
TTGCGCTGCAATACACAGACAAGCCCATAGCCGCGGGTGAAGGTGATTTAAGAGCCGTGCAAGAAAGAGTCAAAGGCTTT
GTCGAAACGGGTAAATTAGGTCCCTTCGCCAACGCTTATTGGGGCAATGGCACCTATAAGTTTACTCCAGAGCAAAACCT
TATCGCGCTATCCCACTACCTTAAAGCACTTGAAGTACAACGTGTTGCGGCGGAAATGCTGGCCATCTTTGGCGGTAAAT
CTCCACATCCACAATCCGTTGTTGTCGGTGGTGTGACGTCGGTGCGTGACATGTTAAGCCCTGCTCGCCTGCAGGAATGG
AAGCAAAAACATGCGACCGTGACTGACTTTATCCTGCGTGCTTATCAAGCGGATATCGTAATGGCGGCCGAAGCCTTTGG
TACTGAGCCAAGCGTGCTAGGCGGCGTGAATGTGAAAAACTTCATGGCGACCGATGACTTTGTGTTAGCCAATGGCGAAT
ACATGTTCAATCAGGGCGTGATCATGAACGGCGACCTTGCCGGTGTCAGCGATGTAAACCCAGATCTGATTGCTGAAGAC
GTCAGCCATGCTTGGTATAGCGCCGATGCGCCGCAGCATCCCTATGACGGTACCACCATCCCCAATTACACTGGTTTTGT
TGAACGCGATACTGTCTATGGCAAGCTGCCAACACTGGATGGCGATGGCAAATACTCTTGGGTGAAATCCCCCCGTTATC
AAGGCGAACCTGTTGAAGTGGGCCCATTAGCCTGCCTATTGGTGAGCTATGCCCGTGGTAACAAAGTGGTTGTGGATTCT
GTCAATGCCTTGCTCGCTCGCACTGGCTTACCCGTTGCCGCCCTATTTACCACTTTAGGTCGCACCGCTGCCCGTATGTT
ACAGACAGTTATCGTCGCCCAAGAAGGATTAAAAACCTTCGATGCGCTGCTGACGAATATCCAGTCTGATGAATCAACTT
ATGTGAAACCCAATATCGACCCGAGCAAAGAGTACATAGGTCACGCCATGATTGAGGCGCCACGCGGCATGTTAAGCCAC
TGGATCCGCATTAAAAATGGAGTGATTGAGAACTATCAAGCCGTGGTACCAACTACCTGGAACGCAGGTCCTGTGGATGC
CAACGGTAAAATGGGTCCCTATGAAGCCTCTTTAATTGGCTTGAAACTAGAAGATCCTACTAAGCCCCTTGAGGTGATAC
GTATTATTCACTCATTTGACCCTTGCATGGCGTGTTCTGTACACGTGATGGATTTTAAAGGTCAGGCATTGAGCGAGTTC
CGTGTCAGCCCCAATGGTCAATAA

Upstream 100 bases:

>100_bases
CGCAGATACTATTGGTGCGGTGATTTTAGGCGCGACTGTTGTGGGCATTGGAGCCCATGCCGTTGCCAGTATTTTTGCCA
AACCGCTGGAGGAATAACCC

Downstream 100 bases:

>100_bases
TCGAGGGAGGGAACGCAAAATGAACCATTCTGCAACCCGCATTCGGACACTGGTTTTTAGTCCGGCAATACGGATCTTCC
ACTGGCTCAGGGCACTATCA

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSKRVVIDPITRIEGHLRIEVEVDENNVISKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGIQIPLNARYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALQYTDKPIAAGEGDLRAVQERVKGF
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSVVVGGVTSVRDMLSPARLQEW
KQKHATVTDFILRAYQADIVMAAEAFGTEPSVLGGVNVKNFMATDDFVLANGEYMFNQGVIMNGDLAGVSDVNPDLIAED
VSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLACLLVSYARGNKVVVDS
VNALLARTGLPVAALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDESTYVKPNIDPSKEYIGHAMIEAPRGMLSH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEF
RVSPNGQ

Sequences:

>Translated_567_residues
MSKRVVIDPITRIEGHLRIEVEVDENNVISKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGIQIPLNARYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALQYTDKPIAAGEGDLRAVQERVKGF
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSVVVGGVTSVRDMLSPARLQEW
KQKHATVTDFILRAYQADIVMAAEAFGTEPSVLGGVNVKNFMATDDFVLANGEYMFNQGVIMNGDLAGVSDVNPDLIAED
VSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLACLLVSYARGNKVVVDS
VNALLARTGLPVAALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDESTYVKPNIDPSKEYIGHAMIEAPRGMLSH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEF
RVSPNGQ
>Mature_566_residues
SKRVVIDPITRIEGHLRIEVEVDENNVISKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENAL
GIQIPLNARYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALQYTDKPIAAGEGDLRAVQERVKGFV
ETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSVVVGGVTSVRDMLSPARLQEWK
QKHATVTDFILRAYQADIVMAAEAFGTEPSVLGGVNVKNFMATDDFVLANGEYMFNQGVIMNGDLAGVSDVNPDLIAEDV
SHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLACLLVSYARGNKVVVDSV
NALLARTGLPVAALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDESTYVKPNIDPSKEYIGHAMIEAPRGMLSHW
IRIKNGVIENYQAVVPTTWNAGPVDANGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEFR
VSPNGQ

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=583, Percent_Identity=45.2830188679245, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1787207, Length=585, Percent_Identity=40.8547008547009, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 62162; Mature: 62031

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVVIDPITRIEGHLRIEVEVDENNVISKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCCEEECCHHHCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGIQIPLNARYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEHEECCCCHHHH
VSALSADPAKAAQVALQYTDKPIAAGEGDLRAVQERVKGFVETGKLGPFANAYWGNGTYK
HHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSVVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCHHHHHHH
KQKHATVTDFILRAYQADIVMAAEAFGTEPSVLGGVNVKNFMATDDFVLANGEYMFNQGV
HHHHCHHHHHHHHHHHHHHEEEHHHCCCCCCEECCCCHHHEECCCCEEEECCCEEECCCE
IMNGDLAGVSDVNPDLIAEDVSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLD
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYSWVKSPRYQGEPVEVGPLACLLVSYARGNKVVVDSVNALLARTGLPVAALFTTLG
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAQEGLKTFDALLTNIQSDESTYVKPNIDPSKEYIGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCC
PCMACSVHVMDFKGQALSEFRVSPNGQ
CHHEEEEEEEECCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SKRVVIDPITRIEGHLRIEVEVDENNVISKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGIQIPLNARYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEHEECCCCHHHH
VSALSADPAKAAQVALQYTDKPIAAGEGDLRAVQERVKGFVETGKLGPFANAYWGNGTYK
HHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKSPHPQSVVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCHHHHHHH
KQKHATVTDFILRAYQADIVMAAEAFGTEPSVLGGVNVKNFMATDDFVLANGEYMFNQGV
HHHHCHHHHHHHHHHHHHHEEEHHHCCCCCCEECCCCHHHEECCCCEEEECCCEEECCCE
IMNGDLAGVSDVNPDLIAEDVSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLD
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYSWVKSPRYQGEPVEVGPLACLLVSYARGNKVVVDSVNALLARTGLPVAALFTTLG
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAQEGLKTFDALLTNIQSDESTYVKPNIDPSKEYIGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCC
PCMACSVHVMDFKGQALSEFRVSPNGQ
CHHEEEEEEEECCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]