Click here to switch to the map view.

The map label for this gene is yeaJ [H]

Identifier: 160874890

GI number: 160874890

Start: 2127699

End: 2129105

Strand: Direct

Name: yeaJ [H]

Synonym: Sbal195_1775

Alternate gene names: 160874890

Gene position: 2127699-2129105 (Clockwise)

Preceding gene: 160874889

Following gene: 160874892

Centisome position: 39.79

GC content: 43.64

Gene sequence:

>1407_bases
ATGGGACATTCGGCACTCAACTGGTTGTTATTACGTCGGTCACTTGCACTTGCGACCCTCTTGTTTGTCATATTCTTGCT
GATCCTATTGCCGTTTAAAATCGATAGAGAACACCAATCCTTTGTGTCTTTTGTAAAAACAAAGCAAATCACACCGAGCT
ATATTGAACTGCTCGGTAAAAGCTTATCCCTGCACGATATCATTGATTTCAATAAATCTTCTTTAGCGCCTTTTCAGCTC
GATCCCACCCGATTAATGCTGCCCCAAGGGGTGGAGCTTTCTGCTGAAGAAGCGCAAGTCTTACGCTACTTTGATTATCT
CTACTCCAGTAAAAAGACAAATGCAGAAGATATTTTCCTGTATGTGGGGCTTTATCGTAGTGATGCTGTGTATTTTGCAA
CCCCAATCCCAGAGGACGTAAAAGCGCGGCTGATTAATGACCGTACTTCGACAAATGAGTTTTGTCAGAGCCTGCACTAT
TGTGCGAAAGACGCGACGCCAGAGTCCTTGGCCGATGGCATAGTGTTATCCATTCCTTATCAAGATGAAGTGACTGGTAA
AGTCATTTTATCTATGGCCTCGCCGATTACATCATCTAGCGATCCTAATCATTTTATTGGTGATGTGATTGCCGACACGG
CCTTTATGACGTCGGGTTTTATGCGTAATAAACATGTGGAATGGCAGAATACAGATTATGGCACTGAGCTGGTGATCACG
ACAGATAGCCTTTGGGCTGAACTGACGCCGGAGTTCTTGTTTTATTCGATGACAAATCATTTAGATAACATGACTTCACT
GACATACAAAATCAGTATATTTCGTTTTATTGTGTGGAATTTGCCCCTGTGGTTTTTGTTATCTATCGTGATCTTCTTCG
TTCAAATTAATATCAATCGTCTTAGGAAACTTCGTACTGAGCGCCAACGGTTTAGTGAAGCGATGTTATTGGATGCTTTT
ACTCGCACCTACAATAAGAAAGTCTATGAGACCACGCTGTTTGATGTCGCAACTAAGGGGCATTACGCTGTGATCTGTGT
GGATGGCGATAAAATCAAAACCATTAATGATACCTACGGGCATAAACTCGGGGACATGGCGATCATGCAAATTGTCGATG
CAATGCGCGGCGTATTTCGAGAAAACGATTTGTTAATCAGAACCGGCGGTGATGAGTTTGTCGTCATCTTAGCTAATTGT
ACTTTTGAACGGGCTTTGGTATTGGCTGAACATTTACGTCAAGCGGTCGCAGAGCGGCAGTTTTTAAATGTGTTGACCGT
TTCCTGCGGTGCCGCTGATTCCCAAGACTATGCAAACTTTGAGGCCGTGCTGCAAGCGGCGGATAAAGCGCTGTATGAGG
ATAAAAAGAATAAGCAGGGTAGCCGTAAGCTCGAAGCGACACGGTAA

Upstream 100 bases:

>100_bases
AATTGCAGTTGTTTTTATTGGTTTATTTTGTGGTTTAATGGGACATTACTTAAGTTTTGGGCAAGGCATATTGTCGGTAT
AACAAGAATTAAGAGGGATT

Downstream 100 bases:

>100_bases
CTATTGTTAGTTATCAGGCTATTTATTGCGTTAACTATTGATATTGAGTTAGCTATTGAGTTAATCATCGAGTGAATTCG
TCACGTGACCGCAATAAAGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGHSALNWLLLRRSLALATLLFVIFLLILLPFKIDREHQSFVSFVKTKQITPSYIELLGKSLSLHDIIDFNKSSLAPFQL
DPTRLMLPQGVELSAEEAQVLRYFDYLYSSKKTNAEDIFLYVGLYRSDAVYFATPIPEDVKARLINDRTSTNEFCQSLHY
CAKDATPESLADGIVLSIPYQDEVTGKVILSMASPITSSSDPNHFIGDVIADTAFMTSGFMRNKHVEWQNTDYGTELVIT
TDSLWAELTPEFLFYSMTNHLDNMTSLTYKISIFRFIVWNLPLWFLLSIVIFFVQININRLRKLRTERQRFSEAMLLDAF
TRTYNKKVYETTLFDVATKGHYAVICVDGDKIKTINDTYGHKLGDMAIMQIVDAMRGVFRENDLLIRTGGDEFVVILANC
TFERALVLAEHLRQAVAERQFLNVLTVSCGAADSQDYANFEAVLQAADKALYEDKKNKQGSRKLEATR

Sequences:

>Translated_468_residues
MGHSALNWLLLRRSLALATLLFVIFLLILLPFKIDREHQSFVSFVKTKQITPSYIELLGKSLSLHDIIDFNKSSLAPFQL
DPTRLMLPQGVELSAEEAQVLRYFDYLYSSKKTNAEDIFLYVGLYRSDAVYFATPIPEDVKARLINDRTSTNEFCQSLHY
CAKDATPESLADGIVLSIPYQDEVTGKVILSMASPITSSSDPNHFIGDVIADTAFMTSGFMRNKHVEWQNTDYGTELVIT
TDSLWAELTPEFLFYSMTNHLDNMTSLTYKISIFRFIVWNLPLWFLLSIVIFFVQININRLRKLRTERQRFSEAMLLDAF
TRTYNKKVYETTLFDVATKGHYAVICVDGDKIKTINDTYGHKLGDMAIMQIVDAMRGVFRENDLLIRTGGDEFVVILANC
TFERALVLAEHLRQAVAERQFLNVLTVSCGAADSQDYANFEAVLQAADKALYEDKKNKQGSRKLEATR
>Mature_467_residues
GHSALNWLLLRRSLALATLLFVIFLLILLPFKIDREHQSFVSFVKTKQITPSYIELLGKSLSLHDIIDFNKSSLAPFQLD
PTRLMLPQGVELSAEEAQVLRYFDYLYSSKKTNAEDIFLYVGLYRSDAVYFATPIPEDVKARLINDRTSTNEFCQSLHYC
AKDATPESLADGIVLSIPYQDEVTGKVILSMASPITSSSDPNHFIGDVIADTAFMTSGFMRNKHVEWQNTDYGTELVITT
DSLWAELTPEFLFYSMTNHLDNMTSLTYKISIFRFIVWNLPLWFLLSIVIFFVQININRLRKLRTERQRFSEAMLLDAFT
RTYNKKVYETTLFDVATKGHYAVICVDGDKIKTINDTYGHKLGDMAIMQIVDAMRGVFRENDLLIRTGGDEFVVILANCT
FERALVLAEHLRQAVAERQFLNVLTVSCGAADSQDYANFEAVLQAADKALYEDKKNKQGSRKLEATR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081974, Length=368, Percent_Identity=26.6304347826087, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=215, Percent_Identity=29.7674418604651, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI145693134, Length=198, Percent_Identity=32.3232323232323, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=155, Percent_Identity=30.3225806451613, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=148, Percent_Identity=34.4594594594595, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082007, Length=287, Percent_Identity=25.4355400696864, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=28.9940828402367, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081977, Length=153, Percent_Identity=28.7581699346405, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53341; Mature: 53210

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHSALNWLLLRRSLALATLLFVIFLLILLPFKIDREHQSFVSFVKTKQITPSYIELLGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCC
SLSLHDIIDFNKSSLAPFQLDPTRLMLPQGVELSAEEAQVLRYFDYLYSSKKTNAEDIFL
CCCHHHHHCCCCCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
YVGLYRSDAVYFATPIPEDVKARLINDRTSTNEFCQSLHYCAKDATPESLADGIVLSIPY
EEEEEECCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEEEECC
QDEVTGKVILSMASPITSSSDPNHFIGDVIADTAFMTSGFMRNKHVEWQNTDYGTELVIT
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
TDSLWAELTPEFLFYSMTNHLDNMTSLTYKISIFRFIVWNLPLWFLLSIVIFFVQININR
ECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHH
LRKLRTERQRFSEAMLLDAFTRTYNKKVYETTLFDVATKGHYAVICVDGDKIKTINDTYG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCEEEECCHHHC
HKLGDMAIMQIVDAMRGVFRENDLLIRTGGDEFVVILANCTFERALVLAEHLRQAVAERQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
FLNVLTVSCGAADSQDYANFEAVLQAADKALYEDKKNKQGSRKLEATR
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GHSALNWLLLRRSLALATLLFVIFLLILLPFKIDREHQSFVSFVKTKQITPSYIELLGK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCC
SLSLHDIIDFNKSSLAPFQLDPTRLMLPQGVELSAEEAQVLRYFDYLYSSKKTNAEDIFL
CCCHHHHHCCCCCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
YVGLYRSDAVYFATPIPEDVKARLINDRTSTNEFCQSLHYCAKDATPESLADGIVLSIPY
EEEEEECCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEEEECC
QDEVTGKVILSMASPITSSSDPNHFIGDVIADTAFMTSGFMRNKHVEWQNTDYGTELVIT
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
TDSLWAELTPEFLFYSMTNHLDNMTSLTYKISIFRFIVWNLPLWFLLSIVIFFVQININR
ECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHH
LRKLRTERQRFSEAMLLDAFTRTYNKKVYETTLFDVATKGHYAVICVDGDKIKTINDTYG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCEEEECCHHHC
HKLGDMAIMQIVDAMRGVFRENDLLIRTGGDEFVVILANCTFERALVLAEHLRQAVAERQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
FLNVLTVSCGAADSQDYANFEAVLQAADKALYEDKKNKQGSRKLEATR
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]