Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 160874887

GI number: 160874887

Start: 2124788

End: 2125432

Strand: Reverse

Name: purN [H]

Synonym: Sbal195_1772

Alternate gene names: 160874887

Gene position: 2125432-2124788 (Counterclockwise)

Preceding gene: 160874888

Following gene: 160874886

Centisome position: 39.75

GC content: 48.99

Gene sequence:

>645_bases
ATGCCCCAGAGCTGTCGTGTAGTTGTATTAATTTCCGGAAATGGCAGTAACCTGCAAGCGATTATCGACGGTTGCGACGA
TAATCTGCAGGCCGAAGTTGTCGGAGTCATCAGTAATAAACCCGATGCCTATGGCCTAGTGCGCGCCCATCATGGCGAAA
TTGATACCAGCTGCGTGATAGCTCACCAAGGTGAATCTCGCTCTGAATACGATGCGCGTTTAATGACAGTCATCGAACAG
TACCAACCCGATTTAATCGTGTTGGCAGGATTTATGCGGATCTTAACCGATGATTTCGTGAATCATTACCTAGGTCGCAT
GATCAATATCCATCCGTCACTGTTACCGAAATACACTGGCTTAAACACCCATCAACGTGCCATTGACGCTAACGACAGCG
AACATGGTGCCAGCGTGCATTTTGTCACGCCAGAGCTTGATGCAGGTCCGGTGATTTTACAGGCTAAAGTGCCTGTTTAT
GAAGAAGATACCGCTGACATGCTCGCCGCCCGTGTGCATGAGCAAGAACATGCCATTTATCCACTCGTGGTGAAATGGTT
TAGTCAGCACCGTCTTAACATGCAAAACGGCCAAGCCTATCTTGATGGCAATCTCATCGGCCCGAGCGGTTACGCGCCTG
ATTAG

Upstream 100 bases:

>100_bases
ATGCTGCACTTGCATTACTGGCTGCAGAAGGCGAACAAGCTTGGCTGATCGGTGCTATCGCAGATCGTGAAGGCAATGAA
GAGCAAGTGGAGATCCTGTA

Downstream 100 bases:

>100_bases
TTTCGCGAAATAGCAATTGGTCGCGAGATCACAAATAGAGACCTTAGGGTCTCTATTTTTTTAACTATTTTCCACAAAAG
CATTGTTTTCTGGGGCAATG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQ
YQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EEDTADMLAARVHEQEHAIYPLVVKWFSQHRLNMQNGQAYLDGNLIGPSGYAPD

Sequences:

>Translated_214_residues
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQ
YQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EEDTADMLAARVHEQEHAIYPLVVKWFSQHRLNMQNGQAYLDGNLIGPSGYAPD
>Mature_213_residues
PQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQY
QPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYE
EDTADMLAARVHEQEHAIYPLVVKWFSQHRLNMQNGQAYLDGNLIGPSGYAPD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=59.3301435406699, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1787483, Length=186, Percent_Identity=30.6451612903226, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=36.7231638418079, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=32.994923857868, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=49.4444444444444, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23584; Mature: 23453

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVI
CCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCEEEE
AHQGESRSEYDARLMTVIEQYQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEEDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
PLVVKWFSQHRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHCCCCCCCCEEECCEEECCCCCCCC
>Mature Secondary Structure 
PQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVI
CCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCEEEE
AHQGESRSEYDARLMTVIEQYQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEEDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
PLVVKWFSQHRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHCCCCCCCCEEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]