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The map label for this gene is asnB [H]

Identifier: 160874884

GI number: 160874884

Start: 2119686

End: 2121350

Strand: Reverse

Name: asnB [H]

Synonym: Sbal195_1769

Alternate gene names: 160874884

Gene position: 2121350-2119686 (Counterclockwise)

Preceding gene: 160874885

Following gene: 160874880

Centisome position: 39.67

GC content: 47.81

Gene sequence:

>1665_bases
ATGTGTTCAATCTTTTCGATTTTAGATATCCAATCTGATGCCAAAGAACTGCGCCAAGTCGCGCTAGAAATGTCTAAGCT
AATGCGCCACCGTGGCCCTGACTGGTCAGGTATTTATGCCAGTGATAAGGCAATTCTTGCCCACGAACGTTTAGCGATTG
TTGACATTGAACACGGTGCTCAGCCGCTACTGACCGAAGATGGCAGTTTGATCCTTGCGGTCAACGGCGAAATCTATAAC
CACAAAGAATTGAAAGCCCAGCTAGGCGATAAGTACAGCTATCAAACCAACTCTGACTGCGAAGTCATTCTGGCGCTGTA
TCAAGAATATGGCACTGACTTCCTTGATAAATTAAACGGTATCTTCGCCTTTGTGTTATATGACAAAGCCCAAGATAGAT
ACCTTATCGGCCGCGATCACATGGGTATCATCCCGCTATACACAGGCCGTGACGCTGCGGGTAACTTCTATGTTGCCTCT
GAAATGAAAGCGCTGATGCCAGTGTGTAAAACCGTAGAAGAGTTCTTACCGGGCCAGTATCTGTATTCAAAAGATGGTGA
AGCCGTTAAATACTATAGCCGCGATTGGCAAAGCTATGATGCGGTTAAAGATAACCCAGCCAGCCAAGAAGAATTACGTG
ACGCATTAGAAGCAGCGGTCAAACGCCAATTAATGTCAGACGTGCCCTATGGTGTGTTGCTATCAGGTGGCTTAGATTCA
TCGATCATTTCCGCCGTGACGCAAACCTTTGCTAAACGCCGCATTGAAGACGATGGCGAAACCAACGCTTGGTGGCCACA
GTTGCATTCTTTTGCTGTCGGCCTTGAAGGTGCGCCAGATTTAATCGCCGCCAAAAAAGTCGCTGATGCCATTGGTACGA
TTCACCATGAGATACATTTCACCTTCCAAGAAGGCTTAGATGCGATTAAAGAAGTGATTTATCACTTAGAAACCTACGAT
GTCACCACCATACGTGCCGCGACACCTATGTATTTAATGGCGCGTAAAATCAAAGCCATGGGCATTAAGATGGTGTTATC
GGGAGAAGGTGCTGATGAGCTATTTGGCGGCTATTTATACTTCCATAAAGCGCCAAACGCACAGGCATTCCACGAGGAAT
TAGTCCGCAAACTCGATAAGCTGCATCTGTTCGATTGTCTGCGTGCCAACAAAGCCATGGCCGCTTGGGGATTAGAAGCC
CGCGTGCCCTTCCTCGATAAAGAGTTTATGGATGTGGCGATGCGCATCAACCCTGAAGCGAAAATGTCTAAAGATGGTCG
TATCGAGAAACACATTCTGCGCCAAGCCTTTGAGCATAAATTGCCAAAAGAAGTGGCGTGGCGTCAAAAGGAACAATTCA
GTGACGGCGTCGGCTACTCTTGGATTGACGGTTTGAAAGAACAAGCTGCAGCGCGGGTGGATGATTTACAATTGGCAAAC
GCGAAATTCCGTTTCCCATACAACACCCCAGAGACCAAAGAAGCCTATTTCTATCGCTGCTTCTTTGAGGAACATTATCC
ATTGAGCGGCGCCGCTGAAACGGTTCCAGGTGGTAAATCCGTCGCCTGTTCAACGCCAGAAGCGCTGGCTTGGGACGAAA
GCTTACGCGGCATTATCGACCCATCGGGCCGAGCAGTACGCTCAGTGCACACCTCAAGTTATTAA

Upstream 100 bases:

>100_bases
ATAAAACATCAAAAATAGTGTTTGGCAAATACCTAAATTGCTGGCACATTAAACCTCAATTGAGACATCCTCAAATGACA
GACAGTGTGGGAGTTGTACT

Downstream 100 bases:

>100_bases
TAGCCTAACGTTGAAGTGCTCAGTTGATAAACTAGGTTAATACGCTAAATTAATAAGCTAAGTGAATCAGCTGGATTGAT
AAACAGGTTAAGATAGCCAA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKAQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY

Sequences:

>Translated_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKAQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY
>Mature_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKAQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=41.0681399631676, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=41.0681399631676, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=41.0681399631676, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=42.6070038910506, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=43.652561247216, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=43.652561247216, Blast_Score=319, Evalue=4e-87,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.3971119133574, Blast_Score=830, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=569, Percent_Identity=42.530755711775, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71993933, Length=531, Percent_Identity=43.6911487758945, Blast_Score=420, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=35.5640535372849, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=312, Percent_Identity=39.1025641025641, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.4587155963303, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=51.0989010989011, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI45553209, Length=578, Percent_Identity=47.2318339100346, Blast_Score=502, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62369; Mature: 62369

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IFAFVLYDKAQDRYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFLPGQY
EEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
LYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTSSY
CCCHHHHHHHCCCC
>Mature Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IFAFVLYDKAQDRYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFLPGQY
EEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
LYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTSSY
CCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]