Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 160874791

GI number: 160874791

Start: 2019338

End: 2020225

Strand: Reverse

Name: gcvA [H]

Synonym: Sbal195_1674

Alternate gene names: 160874791

Gene position: 2020225-2019338 (Counterclockwise)

Preceding gene: 160874796

Following gene: 160874785

Centisome position: 37.78

GC content: 48.65

Gene sequence:

>888_bases
ATGTTGACCGAACTGCCACCACTTAATTCCCTCCGAGCTTTTGAAGCGGCTGCACGCTTGCAGAGCATGACATTAGCCAG
CAAGGAGCTGCATGTCACCCATGGTGCGGTCAGTAAACAAATCCGAGTATTAGAGGATTATTTGGGTTTTGCGCTTTTTG
TGCGCTTACATAAAAAAGTCAGTTTAACCGATGAAGCCAAGCGCTATTTGCCCCATGTCCAAGCCGCGCTGCAAACCCTG
TCAAGTGCAACGAGTGACCTACGCCGCCAGCCTTCTAAGCCACAAACCTTAGCCATCAATGTGCTGCCAAGCTTAACCAT
TAATTGGCTCATCCCGAGAATGGAGCAGTTTAAGTCCCGCCATCCGCACCTTTATGTCGATTTGTCGATTGGTGACTTTG
CCGTGGACTTTAGCCAAGGCCGTTATGATATTGCGATTCGCAGCTGCACAGTCATTCCTAAGGGCGCGAATTATATCAAG
CTGATGGATGAAGATTTGTGTTTAGTTTGCGCGCCATCACTAGCGCCAAAACTGCAAAACATTGAGGATATCAATCACGT
CACCTTACTGAAACACACGACAAGACCCGAATTGTGGGAATATTGGGCAGAAAAAGTGGGCTTAACCCTTACTCAAGCGC
AATCCTTTGGTGTCGAGCATTTCTACATGCTCAGCCAAGCCGCCGCGAGTGGCATGGGGGCAGCGCTTATTCCGCGATTC
TTTATCGAAGATCAATTGGCAAACGGCAGCTTAGTCATTCCCTTCGACGCCCCTTTCACTAGCCCCTATCGATATTACTT
ACTCACGCCCAAGTCCCCTAGCTTGCCGTTAAAAGCCCAAGCCTTTATTGACTGGTTATTAGAAGTGTTTGCCCCTTATC
GCCACTAA

Upstream 100 bases:

>100_bases
GTTTGCCAAATAAACGCAAAGGAGTTATTGTCGCCACTGCGTGAGTTAAACTCACACAAATCTGTAATCCTGTTCGAATC
TCCCAACTGTAGGACATCAA

Downstream 100 bases:

>100_bases
ACAACATTCGGAATGTCACCATTCCAGCAAGGTGCAATAACGCTATATGTGCCAAATAAAGCTATGGAGTACTGAAAATA
AACGCAGCAAATGAGCGCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTVIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQAQSFGVEHFYMLSQAAASGMGAALIPRF
FIEDQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH

Sequences:

>Translated_295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTVIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQAQSFGVEHFYMLSQAAASGMGAALIPRF
FIEDQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
>Mature_295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTVIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQAQSFGVEHFYMLSQAAASGMGAALIPRF
FIEDQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=35.3951890034364, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=31.6151202749141, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=31.3380281690141, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=25.6227758007117, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=23.841059602649, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=165, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=30.5164319248826, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=22.8373702422145, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33264; Mature: 33264

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLTDEAKRYLPHVQAALQTLSSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
HPHLYVDLSIGDFAVDFSQGRYDIAIRSCTVIPKGANYIKLMDEDLCLVCAPSLAPKLQN
CCEEEEEEECCCEEEECCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEECCHHCHHHCC
IEDINHVTLLKHTTRPELWEYWAEKVGLTLTQAQSFGVEHFYMLSQAAASGMGAALIPRF
HHHCCEEEEEEECCCHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
FIEDQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
HHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLTDEAKRYLPHVQAALQTLSSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
HPHLYVDLSIGDFAVDFSQGRYDIAIRSCTVIPKGANYIKLMDEDLCLVCAPSLAPKLQN
CCEEEEEEECCCEEEECCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEECCHHCHHHCC
IEDINHVTLLKHTTRPELWEYWAEKVGLTLTQAQSFGVEHFYMLSQAAASGMGAALIPRF
HHHCCEEEEEEECCCHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
FIEDQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
HHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]