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The map label for this gene is mak [H]

Identifier: 160874338

GI number: 160874338

Start: 1452379

End: 1453275

Strand: Direct

Name: mak [H]

Synonym: Sbal195_1219

Alternate gene names: 160874338

Gene position: 1452379-1453275 (Clockwise)

Preceding gene: 160874337

Following gene: 160874339

Centisome position: 27.16

GC content: 48.94

Gene sequence:

>897_bases
ATGTTACGAATTGGTATCGATCTTGGCGGCACAAAAATCGAGTTAGTCGCATTGGATGACGATGGTAAAGAAGTGTTTCG
TAAACGCATCCCAACGCCAAGAGAATATGTTGCGACAGTCGATGCATTAGTCGCACTCGTGACAGAAGTCGAAGAGAGCT
TGCAACAAACCGCCACAATAGGCATAGGTATTCCAGGGGTGATTTCTCCTTTTACTGGCCTAGTTAAAAATGCTAATTCA
ACGTGGATCAATGGCCATCCCCTCGATGTGGATCTGGGCGCTCGATTAAACCGTAAGGTGAAAATCGCTAATGATGCGAA
CTGTTTTGCCGTATCAGAAGCGGTCGATGGCGCAGCTGCTGGCAAGTCAGTGGTCTTTGGCGCCATTATTGGTACTGGAT
GTGGTGCAGGTATCGCCATCAAAGGCCAAGTGCATGATGGTGGTAATGGCATAGGCGGTGAGTGGGGGCATAATCCTTTA
CCTTGGATGAAAGCCGATGAATTTAATACCACGACGTGTTTTTGTGGTAACAGAGACTGCATCGAGACGTTTATCTCAGG
GACGGGTTTTGTACGCGATTTTCGCGCAGCAGGTGGCGTTGCTGACAGCGGTATTGAAATCGCCCAAATGATGCAAGCGG
GCGATCCGCTGGCAACTCAAGCTTTTGATCGCTTTATCGATCGTTTAGCCCGCTCATTGGCCCATGTGATCAATATGATG
GATCCTGATGTAATCGTCCTAGGCGGCGGAGTGTCCAACATCGATGCCATTTACACTGAATTGCCAAAAGTTTTACCGCT
ATATGTGGTTGGCCGTGAGTGTCACACTCCGGTTGTTAAAAACTGTTATGGCGCTTCATCGGGCGTACGTGGTGCCGCTT
GGTTGTGGGGACGCTAA

Upstream 100 bases:

>100_bases
TGACCTGAGGCAGTTTTCAATTACAGTGTATGGCATCAGGCAGTAGAGCCGAGCTTCACTGAGCCGCTTTGCGTTAAATA
TATTGAGATGGAGTAAGTTG

Downstream 100 bases:

>100_bases
CTTTAGACTTAATTTGAAATAGTGTTTGATATTTAAGGCCGAATTCATTTATTTGAATTTGGCCTTTTTTTGATTTTGTC
ATTCTAAAGTTTTACATTTC

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLRIGIDLGGTKIELVALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPLYVVGRECHTPVVKNCYGASSGVRGAAWLWGR

Sequences:

>Translated_298_residues
MLRIGIDLGGTKIELVALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPLYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
>Mature_298_residues
MLRIGIDLGGTKIELVALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPLYVVGRECHTPVVKNCYGASSGVRGAAWLWGR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=58.5284280936455, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=37.7926421404682, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1790522, Length=298, Percent_Identity=25.503355704698, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=22.5454545454545, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787878, Length=228, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31426; Mature: 31426

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGIDLGGTKIELVALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATI
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
GIGIPGVISPFTGLVKNANSTWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCEEEEECCCCEEEEHHHHCCHHC
GKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPLPWMKADEFNTTTCFCGNRDC
CCHHEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
IETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
HHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
DPDVIVLGGGVSNIDAIYTELPKVLPLYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
CCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHCCCCCCCCCCEECCCC
>Mature Secondary Structure
MLRIGIDLGGTKIELVALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATI
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
GIGIPGVISPFTGLVKNANSTWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCEEEEECCCCEEEEHHHHCCHHC
GKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPLPWMKADEFNTTTCFCGNRDC
CCHHEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
IETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
HHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
DPDVIVLGGGVSNIDAIYTELPKVLPLYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
CCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]