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The map label for this gene is dhfrIII [H]

Identifier: 160874194

GI number: 160874194

Start: 1271791

End: 1272273

Strand: Direct

Name: dhfrIII [H]

Synonym: Sbal195_1074

Alternate gene names: 160874194

Gene position: 1271791-1272273 (Clockwise)

Preceding gene: 160874193

Following gene: 160874195

Centisome position: 23.78

GC content: 44.51

Gene sequence:

>483_bases
ATGCGCATTGCTATGATAGCCGCGATGTCAAACAATCGAGTCATCGGAAAAGAGAATAAAATGCCATGGCATCTTCCAGA
AGATCTACGTCACTTTAAAGCCATGACCCTAGGTAAGCCTGTGCTGATGGGGCGTAAGACCTTTGAGTCCATTGGTAGAC
CCCTTCCTGGTCGACATAACATAGTGATTTCACGTCAAACGGATCTCGTCATTGAAGGCGTGACCTGTGTGACCTCTTTT
GATGCAGCCAAACAAGCCGCTGGCGAGTGTGAAGAGTTAGTGGTGATTGGTGGCGGTCAGCTTTATGCCGAGTTATTACC
TTACGCAGATACCTTATATTTGACGCAAATCAAGCTAGAGGTTGAGGGTGATACATTTTTCCCCCACTGGGATGACGGAA
CTTGGAAAGAAACGGAATCTTTGTCTAGTATAAATGCGGATGGTTTGGAATATCGCTTTATCAATTTGGTAAAAAAATGT
TAA

Upstream 100 bases:

>100_bases
TCTCGGGGCGTTAGCTGTGGGGTTGGCCATGCCGAGCTTGCTGTATTTCAGAACCCGCCCCGTCATTCAATAATTCTATT
TCAATAAAACAGGAGTGAGC

Downstream 100 bases:

>100_bases
ATTGCAGCCTCTGTTGTGTACCCTGTGTGTATGGGTCGTCAGTTTATCTAACGTATTGCATCATATCGGACATTTAGTTT
TCACGGAAAGAAGAGATAGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRIAMIAAMSNNRVIGKENKMPWHLPEDLRHFKAMTLGKPVLMGRKTFESIGRPLPGRHNIVISRQTDLVIEGVTCVTSF
DAAKQAAGECEELVVIGGGQLYAELLPYADTLYLTQIKLEVEGDTFFPHWDDGTWKETESLSSINADGLEYRFINLVKKC

Sequences:

>Translated_160_residues
MRIAMIAAMSNNRVIGKENKMPWHLPEDLRHFKAMTLGKPVLMGRKTFESIGRPLPGRHNIVISRQTDLVIEGVTCVTSF
DAAKQAAGECEELVVIGGGQLYAELLPYADTLYLTQIKLEVEGDTFFPHWDDGTWKETESLSSINADGLEYRFINLVKKC
>Mature_160_residues
MRIAMIAAMSNNRVIGKENKMPWHLPEDLRHFKAMTLGKPVLMGRKTFESIGRPLPGRHNIVISRQTDLVIEGVTCVTSF
DAAKQAAGECEELVVIGGGQLYAELLPYADTLYLTQIKLEVEGDTFFPHWDDGTWKETESLSSINADGLEYRFINLVKKC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=173, Percent_Identity=32.9479768786127, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786233, Length=140, Percent_Identity=55, Blast_Score=148, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=30.8988764044944, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324810, Length=135, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008026, Length=150, Percent_Identity=32.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647458, Length=150, Percent_Identity=32.6666666666667, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17956; Mature: 17956

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAMIAAMSNNRVIGKENKMPWHLPEDLRHFKAMTLGKPVLMGRKTFESIGRPLPGRHN
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCC
IVISRQTDLVIEGVTCVTSFDAAKQAAGECEELVVIGGGQLYAELLPYADTLYLTQIKLE
EEEECCCCEEEECEEEEEEHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCEEEEEEEEEE
VEGDTFFPHWDDGTWKETESLSSINADGLEYRFINLVKKC
ECCCEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRIAMIAAMSNNRVIGKENKMPWHLPEDLRHFKAMTLGKPVLMGRKTFESIGRPLPGRHN
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCC
IVISRQTDLVIEGVTCVTSFDAAKQAAGECEELVVIGGGQLYAELLPYADTLYLTQIKLE
EEEECCCCEEEECEEEEEEHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCEEEEEEEEEE
VEGDTFFPHWDDGTWKETESLSSINADGLEYRFINLVKKC
ECCCEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]