Click here to switch to the map view.

The map label for this gene is cysN [H]

Identifier: 160874083

GI number: 160874083

Start: 1142155

End: 1143597

Strand: Direct

Name: cysN [H]

Synonym: Sbal195_0963

Alternate gene names: 160874083

Gene position: 1142155-1143597 (Clockwise)

Preceding gene: 160874082

Following gene: 160874084

Centisome position: 21.36

GC content: 49.27

Gene sequence:

>1443_bases
ATGGACAAAGCGGTCAATAAAACCAATCTCATGGCGGCAGAAATTACCCTCCATGGTGTGAAAGAATATCTAGCCCTGCA
GCAGCACAAAGGCTTACTGCGCTTTTTAACCTGTGGCAGTGTGGATGACGGCAAAAGTACCTTAATCGGCCGCCTGTTAC
ACGACAGCGCCCAGATCTATGAAGATCAATTGGCGAGCCTAAAGAGCGACAGCGCTAAGATGGGCACCACAGGTGAAGCC
ATCGACTTAGCCTTGCTGGTCGATGGTCTGCAAGCTGAGCGCGAGCAGGGCATCACTATCGATGTGGCTTACCGTTACTT
TTCAAGTGATAAGCGTAAGTTCATCATCGCCGATACGCCAGGGCATGAGCAGTACACGCGCAACATGGCAACGGGTGCAT
CGACCTGTGATTTGGCGGTGATCTTAGTCGATGCCCGCTACGGCGTGCAGACTCAGACTAAACGCCATGCGTTTATCGCG
TCATTACTGGGGATTCGTCACTTCGTTGTTGCCGTGAACAAGATGGATTTGCTTGGGTTTGACGAGCAAGTATTCAATCG
CATTCGTGCTGACTTTACCGATTTCGTTAAAGATTTCGGTAATCTCGATATCCACTTTGTGCCGTTATCGGCGCTTAATG
GTGACAACGTGGTTGAGCGCAGTACGCAAAGCGACTGGTATCAAGGTGGTACTTTGCTTGAACTGCTAGAAACTATTGAT
ACGCAACGTGAACTCAGCTCATTGCCAGTACGTTTTCCTGTGCAATATGTGTCGCGTCCTAACTTAGATTTTCGCGGTTT
TGCAGGCACTTTAGCCTCTGGCATTATCAATGTCGGCGACGAGTTAGTCGCTTTGCCATCGGGCAAACGCAGCAAGGTTG
AACGTATTGTGACCTTCGATGGCGACATACAAGAAGCGGTTGCTGGCCAAGCTGTGACCATCACGCTGGAAGATGAAATT
GATATTTCCCGTGGCGATTTACTGGCAAGACCGGATTCAGCACCCGCCGTCGCTAACGTGATCAGCGCTGACATAGTGTG
GATGGATGAAAAGCCATTACAACTCGGTCAGTTATACGACATCAAAGTGGCCGGTAAGAAGACACAAGCCTCTGTGACTG
CTATCGATTATGTGGTTGATGTTAATACCTTAGCGCGCAGCGCGACGGATACACTTGGGTTAAACGCGATTGCTCGCGTG
ACCTTAGAGCTGACCGAAGACATAGTGTTCGATGCGTATTCATTAGTGCGTGATACGGGCGGAATGATCCTAATCGATCG
CCTGAGCAATGCGACGGTTGCCGCAGTGATGGTGGTGTCGGGTCAGCATGTTAACAAGCAAACAGCATCACAATTTAGTG
CCTTTGAAGTTGAGTTTAATGCCTTAGTGCGTAAACACTACCCACATTGGCAAGCGATAGATATCTCTAAGCTTGGAGCT
TAA

Upstream 100 bases:

>100_bases
GTCAAGGCTACTTCTAATCTCGGCAAGGTTAACCCTATTTCGCGAAGGTGGCGCAAGCAAAGCGCGACCTTCAGCAAAGC
AGATTAAGAGTGAGAATAAG

Downstream 100 bases:

>100_bases
TTTGTGAGTGACTTGTGGCTGTTAGCGATTATTTTGTTAGGTCTTGTGGCTGGCTTAATTAGCGGCCGTATCAATCCAGC
GGTATTGTTTTTATTTGCAT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLARPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA

Sequences:

>Translated_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLARPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
>Mature_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLARPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=29.1375291375291, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.4663573085847, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=336, Percent_Identity=29.4642857142857, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI194018522, Length=418, Percent_Identity=27.7511961722488, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI194018520, Length=418, Percent_Identity=27.7511961722488, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI194097354, Length=418, Percent_Identity=27.7511961722488, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI4503471, Length=360, Percent_Identity=28.6111111111111, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI46094014, Length=418, Percent_Identity=27.2727272727273, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI34147630, Length=314, Percent_Identity=29.9363057324841, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI157426893, Length=278, Percent_Identity=26.2589928057554, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789108, Length=460, Percent_Identity=63.695652173913, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790412, Length=314, Percent_Identity=27.7070063694268, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789737, Length=314, Percent_Identity=27.7070063694268, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI2367247, Length=256, Percent_Identity=27.34375, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566629, Length=429, Percent_Identity=27.039627039627, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17552884, Length=360, Percent_Identity=30.5555555555556, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=360, Percent_Identity=30.5555555555556, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=317, Percent_Identity=30.5993690851735, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=317, Percent_Identity=30.5993690851735, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=353, Percent_Identity=28.8951841359773, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=235, Percent_Identity=27.6595744680851, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=352, Percent_Identity=24.7159090909091, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=334, Percent_Identity=29.6407185628743, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=334, Percent_Identity=29.6407185628743, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=408, Percent_Identity=29.9019607843137, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=314, Percent_Identity=28.343949044586, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324761, Length=339, Percent_Identity=26.2536873156342, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=178, Percent_Identity=32.0224719101124, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=26.0969976905312, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24652838, Length=316, Percent_Identity=29.4303797468354, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137572, Length=316, Percent_Identity=29.4303797468354, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553807, Length=270, Percent_Identity=31.1111111111111, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45553816, Length=270, Percent_Identity=31.1111111111111, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24651721, Length=270, Percent_Identity=31.1111111111111, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI17864154, Length=270, Percent_Identity=31.1111111111111, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI17137380, Length=424, Percent_Identity=25.4716981132075, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI281363316, Length=315, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17864358, Length=315, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921738, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52542; Mature: 52542

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHH
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFD
HHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDIQEAVAGQAVTITLEDEIDISRGDLLARPDSAPAVANVISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCCCCCCCEEE
IKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHCCCCCEEECHHHCCC
>Mature Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHH
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFD
HHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDIQEAVAGQAVTITLEDEIDISRGDLLARPDSAPAVANVISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCCCCCCCEEE
IKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHCCCCCEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]