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The map label for this gene is cysH [H]

Identifier: 160874075

GI number: 160874075

Start: 1131907

End: 1132689

Strand: Direct

Name: cysH [H]

Synonym: Sbal195_0955

Alternate gene names: 160874075

Gene position: 1131907-1132689 (Clockwise)

Preceding gene: 160874074

Following gene: 160874076

Centisome position: 21.17

GC content: 48.4

Gene sequence:

>783_bases
ATGGTTGATCATCAACAAGAAAACAGTGATACAGTGCCGACTGTGGTCAGCACGGCCGAGTTAACGGCGCTATTGAGTGC
GCCCAAGGCCGAGCAAACGATAGAGCTTGAGCGCATAAATCAGTTCTTAGCCGGATTGAGCGCACAAGAGCGAGTGTTAT
GGGGCTTAACTTATTTGCCCGGTAATCATGCGCTGTCGTCAAGTTTTGGTATTCAAGCCGCAGTGATGCTGTATATGGTG
AGCCAGGCACAGGCGAATATTCCGGTTATCCTCACGGATACAGGGTATTTGTTCCCTGAAACCTATCAGTTTATCGATGA
GCTGACACAGCGTTTATCACTGAATGTAAAAGTATATCAAGCGCCGATGACAGCCGCATGGCAAGAGGCACGCTTTGGTA
AGTTGTGGGAACAAGGGTTAGACGGGTTAGAGCAATACAACCGTTTAAACAAAGTTGAGCCTATGCAACGCGCCTTAGCC
GAGCTTGACGTTGGTACTTGGTTTGCAGGGCTGAGACGTAGCCAAGCCACCACCCGTGAGGCGCTGCCGATACTGGCGAT
TCATGGCACTCGCTTTAAGTTACTGCCGATTATCGAGTGGTCGAATAAAGATGTGCACCTATATTTGACTCAATTTGACT
TGCCGTACCACCCACTTTGGGACCAAGGCTATGTTTCTGTTGGTGATACCCATTCGAGTAAACCACTTGAATTAGGCATG
ACAGAGGAAGAAACTCGCTTTAATGGGCTGAAACGTGAGTGTGGTCTGCATTACGATATCTAG

Upstream 100 bases:

>100_bases
GGCCGCTACGCCGTCGAGCGTGATGCAGGCGAAACCTTTGGTAACTTCACCGTCCGCGTGGGCGTTGTGAAGGCGGTTAT
TGATGCCGCTAAGGATTTTC

Downstream 100 bases:

>100_bases
TTATCTTATTGATTTAAAGTATTAAACGCCTCAATTGCCATTGGGCGTTTTTTATCTCGGTAAGAAAGGTCTTATCTTTG
GTTGAATTTAATACTCTGGA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVDHQQENSDTVPTVVSTAELTALLSAPKAEQTIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLYMV
SQAQANIPVILTDTGYLFPETYQFIDELTQRLSLNVKVYQAPMTAAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELDVGTWFAGLRRSQATTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI

Sequences:

>Translated_260_residues
MVDHQQENSDTVPTVVSTAELTALLSAPKAEQTIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLYMV
SQAQANIPVILTDTGYLFPETYQFIDELTQRLSLNVKVYQAPMTAAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELDVGTWFAGLRRSQATTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI
>Mature_260_residues
MVDHQQENSDTVPTVVSTAELTALLSAPKAEQTIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLYMV
SQAQANIPVILTDTGYLFPETYQFIDELTQRLSLNVKVYQAPMTAAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELDVGTWFAGLRRSQATTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=242, Percent_Identity=61.1570247933884, Blast_Score=314, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6325425, Length=243, Percent_Identity=31.6872427983539, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29427; Mature: 29427

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHQQENSDTVPTVVSTAELTALLSAPKAEQTIELERINQFLAGLSAQERVLWGLTYLP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
GNHALSSSFGIQAAVMLYMVSQAQANIPVILTDTGYLFPETYQFIDELTQRLSLNVKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEE
APMTAAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALAELDVGTWFAGLRRSQATTRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYDI
CCHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure
MVDHQQENSDTVPTVVSTAELTALLSAPKAEQTIELERINQFLAGLSAQERVLWGLTYLP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
GNHALSSSFGIQAAVMLYMVSQAQANIPVILTDTGYLFPETYQFIDELTQRLSLNVKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEE
APMTAAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALAELDVGTWFAGLRRSQATTRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYDI
CCHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]