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The map label for this gene is speE [H]

Identifier: 160874044

GI number: 160874044

Start: 1088701

End: 1090425

Strand: Reverse

Name: speE [H]

Synonym: Sbal195_0924

Alternate gene names: 160874044

Gene position: 1090425-1088701 (Counterclockwise)

Preceding gene: 160874045

Following gene: 160874043

Centisome position: 20.39

GC content: 50.2

Gene sequence:

>1725_bases
ATGCAAGAAACCAGCTTAACCCCCTCGGCGGCTAAACCTAGCCGCCTAGGTTGGTTCGACGATGTACTCTTATTGGGCAT
CATGGCCGTATTAGCAGGCTGCGGCTTGATTTACGAATATCTTTTATCCCACTATGCGGGGCGCATTTTAGGCGCATTAG
AAGCCGCCATCTACACCATGATTGGCTTGATGATAGTCTCCATGGGACTAGGTGCCTTCGCCGCCCGTAAGATCCGTGAT
GCCTTTACCGGTTTTGTGATCTTAGAACTCACAGTCGCACTCTGCGGCTCACTCGCCATTTTAATCACAGCGGCGGTGAT
TGGTTTCGGCCAACAACTGCCCTTGATGATTGCCAACACCTTAGGTTTACCACCAGATCAACTGCCCGAAGGCGGCATGA
TAGGCTCGCTGCAAAAACTCAGCGAATACTTGCCCTATGTGTGGGGCGTGTTACTGGGCCTGATGATTGGTATGGAAATC
CCACTGATCGCCAGAGTGCGCCAATCACTCTCTGACGAGCATTTGCTGCACAATGCGGGGACAATTTACGGCGCCGATTA
CATAGGCGCTGGCATAGGGGCCGCCATTTGGGTGGGCTTTATGCTCGCCATCGATATCCAACTTGCCGCCGCCTTAACCG
CCAGTTTCAACCTGTTGGCGGGCTTTGTGTTTATCTGGCGCTTTTGGCCTAAAATCCGCCGCCCTAAACTGCTTTTAACT
GGGCATTTTATCGTGACTGGCATTTTACTCGTGTTAGCCATCCACGGCCCACGCTGGGAGCAAGAATTTAATAATTTGCT
CTATAAAGACAAAGTGGTTTACGCCAAGGCGACACGTTTCCAACAGTTGACCTTCACCGAGCGACTGCGCGGTAACGGTC
TGTCGCCCGTTTATGCGTTATACATCAATGGTCGCTTACAGTTTTCTAGCATAGACGAACACATTTACCATGCGTTTCTC
GTGCACCCCACACTCGCCGCCAGCGCCCGTCATAACAAGGTGTTGATCATTGGTGGCGGCGATGGACTCGGTCTTAAGCA
AGTGCTGCGCTGGGAGCCTGAGCAAGTGACGCTGCTCGATCTCGATGTCGGCCTAGTGCAATTGTTTAAGTCGCCTGATA
CAGATATGCCCGAGCGTTTGAGCCGCGCCATGTTGACGCTCAACGGCAATGCGCTAAACGATCCCCGTGTCACTGTGATC
AACGACGATGCATTTAATGGCGTCGATAAGCTGATTGCCACAGGCGTGAAATACGATGCGATTATCGTGGACTTGCCCGA
TCCGAGCCATCCCGATCTTAATAAGCTGTATTCGGATTATTTCTACCGTAAATTAAAGGAACTTATGAGTAGTGATGGTG
CTCTGACTGTACAGTCAACATCGCCTTACCATGCTCCTAAGGCCTTTATTTCGGTAGCAAAAACCTTGGCATTGGCAGGA
TTTGACGTCAAACAGTACCACCATAATGTGCCCAGTTTTGGCGAATGGGGCTGGAGTATCGCCACCGCAGGCGGACTCGA
TGCACAACACAGATTAGCTAAGTTAACTGAGCTGCCCATCAAAGATGACTGGCTCACCTTAGGTTTAGTGGATGGCGCCT
TTGAGTTTCCAGCAAATTTTTATCAAGATGCCGCCAAAATAAAACCCAATGAATTGGGTTCGTTGCAGCTGTATCACTAC
CATTTACAAGATTGGTCCGAGAATCAGGGACTGAATCTTTTTTAA

Upstream 100 bases:

>100_bases
GATGAAGTGGAAAGACAACACAATATCGGCATCGCCGCCGTTGAAATGGCGATCAGTATCGCTATCGCACTTATCCTCAC
GGCACTGATGGCCTAATCGA

Downstream 100 bases:

>100_bases
TAGGATGAGATAGTGCTTGACATATTATGTCTCAGTGCTATCTTTGAACACAGACATAATATGTCAATAAGGACAAATAT
GAATATCCATACCATTGCCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MQETSLTPSAAKPSRLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARKIRD
AFTGFVILELTVALCGSLAILITAAVIGFGQQLPLMIANTLGLPPDQLPEGGMIGSLQKLSEYLPYVWGVLLGLMIGMEI
PLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIRRPKLLLT
GHFIVTGILLVLAIHGPRWEQEFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPVYALYINGRLQFSSIDEHIYHAFL
VHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDVGLVQLFKSPDTDMPERLSRAMLTLNGNALNDPRVTVI
NDDAFNGVDKLIATGVKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSSDGALTVQSTSPYHAPKAFISVAKTLALAG
FDVKQYHHNVPSFGEWGWSIATAGGLDAQHRLAKLTELPIKDDWLTLGLVDGAFEFPANFYQDAAKIKPNELGSLQLYHY
HLQDWSENQGLNLF

Sequences:

>Translated_574_residues
MQETSLTPSAAKPSRLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARKIRD
AFTGFVILELTVALCGSLAILITAAVIGFGQQLPLMIANTLGLPPDQLPEGGMIGSLQKLSEYLPYVWGVLLGLMIGMEI
PLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIRRPKLLLT
GHFIVTGILLVLAIHGPRWEQEFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPVYALYINGRLQFSSIDEHIYHAFL
VHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDVGLVQLFKSPDTDMPERLSRAMLTLNGNALNDPRVTVI
NDDAFNGVDKLIATGVKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSSDGALTVQSTSPYHAPKAFISVAKTLALAG
FDVKQYHHNVPSFGEWGWSIATAGGLDAQHRLAKLTELPIKDDWLTLGLVDGAFEFPANFYQDAAKIKPNELGSLQLYHY
HLQDWSENQGLNLF
>Mature_574_residues
MQETSLTPSAAKPSRLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARKIRD
AFTGFVILELTVALCGSLAILITAAVIGFGQQLPLMIANTLGLPPDQLPEGGMIGSLQKLSEYLPYVWGVLLGLMIGMEI
PLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIRRPKLLLT
GHFIVTGILLVLAIHGPRWEQEFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPVYALYINGRLQFSSIDEHIYHAFL
VHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDVGLVQLFKSPDTDMPERLSRAMLTLNGNALNDPRVTVI
NDDAFNGVDKLIATGVKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSSDGALTVQSTSPYHAPKAFISVAKTLALAG
FDVKQYHHNVPSFGEWGWSIATAGGLDAQHRLAKLTELPIKDDWLTLGLVDGAFEFPANFYQDAAKIKPNELGSLQLYHY
HLQDWSENQGLNLF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786312, Length=296, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323175, Length=244, Percent_Identity=25.8196721311475, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 63151; Mature: 63151

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]