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The map label for this gene is gluQ [H]

Identifier: 160874004

GI number: 160874004

Start: 1044596

End: 1045510

Strand: Reverse

Name: gluQ [H]

Synonym: Sbal195_0883

Alternate gene names: 160874004

Gene position: 1045510-1044596 (Counterclockwise)

Preceding gene: 160874005

Following gene: 160874003

Centisome position: 19.55

GC content: 49.95

Gene sequence:

>915_bases
ATGGCGACTTTATCTTCTCCAACTTCAATTTATCGCCCCTATGTCGGCCGTTTTGCGCCTTCGCCTTCGGGTGCCTTGCA
CTTTGGCTCGCTTATCGCCGCCCTTGGCAGTTATCTGCGTGCGCGGTCGCAACAGGGTAAATGGTTGATTCGCATTGAAG
ACATCGATCCTCCACGGGAAGTCAAAGGCGCCGCCGATGATATCTTGCGCACCTTAGAAGCCTATGGATTTGAGTGGGAT
GATGTGCCTCTGTATCAGAGCCAGCGCACTGCTGCCTATCAAGCACAAATTGATTCGCTGCTCGCCCAAGATAAAGCTTA
TTTCTGCCAATGTAGTCGCAAGCAAATTCAAGCCATGGGCGGCGTCTACGATGGCCGCTGTCACAGCTTGCCACAAACCC
AAGCTGCAAATGTGGTTAAGTCTGGGGCGGTGCGGATAGTTAATACCGCGCAAGTGCAGCAATTTGACGATAACCTCATG
GGGCATATTTGCGTCGATAGCCATTTTGCCGCCGAGGATTTCATTATCAAACGCAGCGATGGTCTGTACGCCTATCAATT
AGCCGTGGTGCTCGACGATGCTTATCAAGGCATCACTGAAGTGGTTCGAGGTTGTGATTTAATCGAAGCCAGTTGTCGCC
AACTGAGTTTGTATCAAAATTTTGACTTAGCGGCACCGCAATGGTTGCACTTGCCACTCGCCTGTTTAAGTAAAGGTTTT
AAGCTCTCAAAACAAAATCATGCACAGGCAATTGATACGCTTAATCCGCAGTCGAGCCTCAATGCGGCGCTGGCATTTTT
AGGCCAACAACCCGTTGAGACCCAAGGTTCTGTGGCGCAAATGCTCGCTCAAGCCGTAGATCAGTTTCAATTATCCACTA
TACCGCAACAATCTGAGATACTGATCGAGCCCTGA

Upstream 100 bases:

>100_bases
TTGAAGCCAAGCTCACTCCAATTTACCCCGATTCGCTTTTATCCTTGTATCCCTCTAAAATACGCCCACTTTGACTGACA
CTAGCTGCTAACTCAGTACT

Downstream 100 bases:

>100_bases
TATACTCATTAGGCCGCATTTGGTATATGATAGCCGCCAGATTTTTAATCAAATAACAGATCATATTTCGAGGTGTCCCA
TTTTTCGCCGTATCAGCCAA

Product: glutamate--tRNA ligase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWD
DVPLYQSQRTAAYQAQIDSLLAQDKAYFCQCSRKQIQAMGGVYDGRCHSLPQTQAANVVKSGAVRIVNTAQVQQFDDNLM
GHICVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFDLAAPQWLHLPLACLSKGF
KLSKQNHAQAIDTLNPQSSLNAALAFLGQQPVETQGSVAQMLAQAVDQFQLSTIPQQSEILIEP

Sequences:

>Translated_304_residues
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWD
DVPLYQSQRTAAYQAQIDSLLAQDKAYFCQCSRKQIQAMGGVYDGRCHSLPQTQAANVVKSGAVRIVNTAQVQQFDDNLM
GHICVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFDLAAPQWLHLPLACLSKGF
KLSKQNHAQAIDTLNPQSSLNAALAFLGQQPVETQGSVAQMLAQAVDQFQLSTIPQQSEILIEP
>Mature_303_residues
ATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWDD
VPLYQSQRTAAYQAQIDSLLAQDKAYFCQCSRKQIQAMGGVYDGRCHSLPQTQAANVVKSGAVRIVNTAQVQQFDDNLMG
HICVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFDLAAPQWLHLPLACLSKGFK
LSKQNHAQAIDTLNPQSSLNAALAFLGQQPVETQGSVAQMLAQAVDQFQLSTIPQQSEILIEP

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=310, Percent_Identity=32.5806451612903, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI226510925, Length=288, Percent_Identity=54.8611111111111, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1788743, Length=260, Percent_Identity=34.6153846153846, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=315, Percent_Identity=30.1587301587302, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=33.4507042253521, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=33.7349397590361, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 33445; Mature: 33314

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPRE
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VKGAADDILRTLEAYGFEWDDVPLYQSQRTAAYQAQIDSLLAQDKAYFCQCSRKQIQAMG
HCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVYDGRCHSLPQTQAANVVKSGAVRIVNTAQVQQFDDNLMGHICVDSHFAAEDFIIKRSD
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCEEEECCC
GLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFDLAAPQWLHLPLACLSKGF
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCC
KLSKQNHAQAIDTLNPQSSLNAALAFLGQQPVETQGSVAQMLAQAVDQFQLSTIPQQSEI
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LIEP
EECC
>Mature Secondary Structure 
ATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPRE
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VKGAADDILRTLEAYGFEWDDVPLYQSQRTAAYQAQIDSLLAQDKAYFCQCSRKQIQAMG
HCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVYDGRCHSLPQTQAANVVKSGAVRIVNTAQVQQFDDNLMGHICVDSHFAAEDFIIKRSD
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCEEEECCC
GLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFDLAAPQWLHLPLACLSKGF
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCC
KLSKQNHAQAIDTLNPQSSLNAALAFLGQQPVETQGSVAQMLAQAVDQFQLSTIPQQSEI
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LIEP
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]