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The map label for this gene is pcnB [H]

Identifier: 160874003

GI number: 160874003

Start: 1043035

End: 1044576

Strand: Reverse

Name: pcnB [H]

Synonym: Sbal195_0882

Alternate gene names: 160874003

Gene position: 1044576-1043035 (Counterclockwise)

Preceding gene: 160874004

Following gene: 160874002

Centisome position: 19.53

GC content: 49.03

Gene sequence:

>1542_bases
TTGGTATATGATAGCCGCCAGATTTTTAATCAAATAACAGATCATATTTCGAGGTGTCCCATTTTTCGCCGTATCAGCCA
ATTCTGCAAACAGCTATTTGAAGACGCGCCTAAAACGACCGCTCCGACTCCAGCTCAGATTGAACCAACAACGCCTGAAC
AAAGTTCTGGAGCGTTAAGCTTAGAGTTAGTGCCAAGAGATGCCCATACCATCTCGCGCAAACAAATCAGCGAAAATGCC
CTTAAGGTGTTATACAGACTGAATAAATCAGGTTTTAAAGCCTATTTAGTCGGCGGCGGTGTACGCGATTTACTCTTAGG
GATTGAACCGAAAGATTTCGACGTAGTGACCAATGCGACACCGGAAGAAATCAAAAAACTGTTCCGCAACTGCCGAGTCG
TTGGCCGTCGTTTCCGTCTGGCCCATATTGTTTTCGGCCGCGATGTTATCGAAGTGGCGACCTTTCGTGGTCACCATGGC
GAAAGCAATGAGAAAGTCTCTAAAGCCAATGCCGAAGGGCGTTTGCTACGTGATAACGTTTATGGCGAAATCGACGAAGA
TGCCGAGCGCCGCGATTTTACCGTCAATGCCCTCTATTACGATATCAGCGATTACTCGATCCGCAGCTATGGCGGCGGTA
TGGCCGATCTCAAAGCGGGTACGTTGAGATTAATTGGCGATCCTGAAACCCGTTACCGTGAAGATCCGGTGCGTATGTTA
AGGGCGGTGCGTTTCGCCACTAAACTCAACATGGGTATCGAAACAGTAACAGCTGCGCCGATTAAACAATTAGCGCCACT
GCTAAAAGATATTCCCGCAGCGCGTATGTACGAAGAAGTGCTGAAACTCTTCTTTGCAGGCAAAGCCTTAGCCAACTTCG
AACTGATGCAAGAATACAAGCTATTCGCGCCGCTGTTTCCGCAAGTGGCCGCTCTGCTAAAAGATGATCCTAAAGGCACG
ACCATGAAAATGGTGCAAGCCATTATGAAGAGCACAGATCAAAGAGTTAACGAAGATAAGCCGGTGACGCCTTCATTCTT
CTATGCTGCGCTTCTCTGGTATCCACTGCGTCAACGCGCCGAAGATATTGCCGTTGAAAGTGGCCTGACGTTATATGATG
CCTTCTTTGCTGCTATGGGCGATGTGATGGAGCAGCAATGCCAAACCATTAGCATTCCACGCCGTTTTAGTACGCCAGCA
AAAGACATTTGGCAGTTACAGTTACGCTTTGACCGTAGCCAAGGCACTCGTGCTTTTAAACTAATGGAACACCCTAAGTT
TAGAGCCGCATACGATTTGCTATTGCTCAGGGGCGAAGTCGAGGCGGGTAATGTCGCTAAGAGTGCCGCTTGGTGGCAAC
AGTTTGTTGAAGCCGATGAAACTGAACGCTTAGCCATTGCGCGTAGCGGCAGTAAATCGCCCAATCGTAACCGTAATGCC
ACTCAACGTCGTCGCCGTCCTCGCGCACCGAATGCAGATGGTTCAGCCGCGACGAGCGCACCTAAAACAAGTGGCACGCC
AAAAGCGAAAGCGGCTGAATAA

Upstream 100 bases:

>100_bases
GCGCAAATGCTCGCTCAAGCCGTAGATCAGTTTCAATTATCCACTATACCGCAACAATCTGAGATACTGATCGAGCCCTG
ATATACTCATTAGGCCGCAT

Downstream 100 bases:

>100_bases
TGCCTCGTGTCTACGTCGCATTAGGCGCAAATCTTGAAGATCCCAAGGCTCAATTGGACAATGCCGTCACGGCATTGTCC
GCATTAGCCTTAGGCGATAG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MVYDSRQIFNQITDHISRCPIFRRISQFCKQLFEDAPKTTAPTPAQIEPTTPEQSSGALSLELVPRDAHTISRKQISENA
LKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHG
ESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMADLKAGTLRLIGDPETRYREDPVRML
RAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFAPLFPQVAALLKDDPKGT
TMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPA
KDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGSKSPNRNRNA
TQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE

Sequences:

>Translated_513_residues
MVYDSRQIFNQITDHISRCPIFRRISQFCKQLFEDAPKTTAPTPAQIEPTTPEQSSGALSLELVPRDAHTISRKQISENA
LKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHG
ESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMADLKAGTLRLIGDPETRYREDPVRML
RAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFAPLFPQVAALLKDDPKGT
TMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPA
KDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGSKSPNRNRNA
TQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE
>Mature_513_residues
MVYDSRQIFNQITDHISRCPIFRRISQFCKQLFEDAPKTTAPTPAQIEPTTPEQSSGALSLELVPRDAHTISRKQISENA
LKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHG
ESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMADLKAGTLRLIGDPETRYREDPVRML
RAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFAPLFPQVAALLKDDPKGT
TMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPA
KDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGSKSPNRNRNA
TQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=213, Percent_Identity=29.5774647887324, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI87081691, Length=438, Percent_Identity=54.337899543379, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=26.865671641791, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281360112, Length=290, Percent_Identity=27.2413793103448, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357337, Length=290, Percent_Identity=27.2413793103448, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57826; Mature: 57826

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYDSRQIFNQITDHISRCPIFRRISQFCKQLFEDAPKTTAPTPAQIEPTTPEQSSGALS
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LELVPRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNAT
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCC
PEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCC
YGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMADLKAGTLRLIGDPETRYREDPVRML
CCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCEEEECCCHHHHCCCHHHHH
RAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAPLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHH
LMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGSKSPNRNRNA
HHHCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
TQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVYDSRQIFNQITDHISRCPIFRRISQFCKQLFEDAPKTTAPTPAQIEPTTPEQSSGALS
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LELVPRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNAT
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCC
PEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCC
YGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMADLKAGTLRLIGDPETRYREDPVRML
CCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCEEEECCCHHHHCCCHHHHH
RAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAPLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHH
LMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGSKSPNRNRNA
HHHCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
TQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]