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The map label for this gene is rsmE [H]

Identifier: 160873963

GI number: 160873963

Start: 987034

End: 987765

Strand: Reverse

Name: rsmE [H]

Synonym: Sbal195_0841

Alternate gene names: 160873963

Gene position: 987765-987034 (Counterclockwise)

Preceding gene: 160873964

Following gene: 160873962

Centisome position: 18.47

GC content: 50.82

Gene sequence:

>732_bases
ATGAGAGTCCCAAGAATTTATCAACCTCAGCCATTAGCCATCAATCAACAGCTGAACTTAGACGAAGATGGCGCGGCCCA
TATCGGCAAAGTGCTGCGCATGGGTAACGGCGAACATATCAGCCTATTCAATGGCGATGGCCATGATTATCTTGCTGAAA
TTGTCGATGCAGGTAAAAAGCATGTCACAGTAAAACTGCTTTCCTGTGAGGCAAATTCATCTGAGTCGCCACTCAACTTG
CACTTAGGCCAAGTGATTTCCCGTGGCGATAGAATGGAATTCACCATTCAAAAATCGGTCGAGCTTGGGGTAAATACCAT
TACGCCGCTGTTTTCCGATCGCTGCGGCGTAAAACTCAATGGCGAACGCCTAGAAAAGAAAATTCAACAATGGCAGAAAA
TCGTCATTAGTGCCTGTGAGCAATCGGGCCGCAGCCAAGTGCCCGTGGTTCGCCCGGCGATGGACTTGCATGATTGGTGC
AGTGAGCCGACCTCGGCGCTAAAACTCAATCTGCACCCAAGGGCCGCACACGGTATCAATGGCTTAGATTTAGCCCATAC
CCGCGTACGCCTATTAATCGGCCCTGAGGGCGGTTTGTCGGCGGAAGAAATCGCCATGACGGAAACCTATCAATTTACCG
ATGTATTGCTTGGCCCCAGAGTGCTGCGCACCGAAACCGCCTCGCTCACCGCCATTACGGCGCTGCAACTCAGATTTGGT
GATTTAGGCTGA

Upstream 100 bases:

>100_bases
CGATAACGCTATAGCGCAAACTCAAGGCAACCATAATCCCTTTGTGCAAAATGCCTGTAAGGCGCCACCACTGCGTCAAC
AACTAGCCGAGTAAGGTCGA

Downstream 100 bases:

>100_bases
TCGACCCAAGCAAACAGATAATCAATGCATTTTTGCCTGCAAACGGCGACAAATAAGGAACAGATTAATGATAAAACTCG
GCATAGTGATGGACCCCATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRVPRIYQPQPLAINQQLNLDEDGAAHIGKVLRMGNGEHISLFNGDGHDYLAEIVDAGKKHVTVKLLSCEANSSESPLNL
HLGQVISRGDRMEFTIQKSVELGVNTITPLFSDRCGVKLNGERLEKKIQQWQKIVISACEQSGRSQVPVVRPAMDLHDWC
SEPTSALKLNLHPRAAHGINGLDLAHTRVRLLIGPEGGLSAEEIAMTETYQFTDVLLGPRVLRTETASLTAITALQLRFG
DLG

Sequences:

>Translated_243_residues
MRVPRIYQPQPLAINQQLNLDEDGAAHIGKVLRMGNGEHISLFNGDGHDYLAEIVDAGKKHVTVKLLSCEANSSESPLNL
HLGQVISRGDRMEFTIQKSVELGVNTITPLFSDRCGVKLNGERLEKKIQQWQKIVISACEQSGRSQVPVVRPAMDLHDWC
SEPTSALKLNLHPRAAHGINGLDLAHTRVRLLIGPEGGLSAEEIAMTETYQFTDVLLGPRVLRTETASLTAITALQLRFG
DLG
>Mature_243_residues
MRVPRIYQPQPLAINQQLNLDEDGAAHIGKVLRMGNGEHISLFNGDGHDYLAEIVDAGKKHVTVKLLSCEANSSESPLNL
HLGQVISRGDRMEFTIQKSVELGVNTITPLFSDRCGVKLNGERLEKKIQQWQKIVISACEQSGRSQVPVVRPAMDLHDWC
SEPTSALKLNLHPRAAHGINGLDLAHTRVRLLIGPEGGLSAEEIAMTETYQFTDVLLGPRVLRTETASLTAITALQLRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=66.6666666666667, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26684; Mature: 26684

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPRIYQPQPLAINQQLNLDEDGAAHIGKVLRMGNGEHISLFNGDGHDYLAEIVDAGKK
CCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
HVTVKLLSCEANSSESPLNLHLGQVISRGDRMEFTIQKSVELGVNTITPLFSDRCGVKLN
EEEEEEEEECCCCCCCCCEEHHHHHHCCCCCEEEEEECCHHCCCHHHCHHHCCCCCCEEC
GERLEKKIQQWQKIVISACEQSGRSQVPVVRPAMDLHDWCSEPTSALKLNLHPRAAHGIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHCCCCCEEEEECCCCHHCCCC
GLDLAHTRVRLLIGPEGGLSAEEIAMTETYQFTDVLLGPRVLRTETASLTAITALQLRFG
CCEECCEEEEEEECCCCCCCHHHEEEHHHHHHHHHHHCCEEEEECCCHHEEEEEEEEECC
DLG
CCC
>Mature Secondary Structure
MRVPRIYQPQPLAINQQLNLDEDGAAHIGKVLRMGNGEHISLFNGDGHDYLAEIVDAGKK
CCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
HVTVKLLSCEANSSESPLNLHLGQVISRGDRMEFTIQKSVELGVNTITPLFSDRCGVKLN
EEEEEEEEECCCCCCCCCEEHHHHHHCCCCCEEEEEECCHHCCCHHHCHHHCCCCCCEEC
GERLEKKIQQWQKIVISACEQSGRSQVPVVRPAMDLHDWCSEPTSALKLNLHPRAAHGIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHCCCCCEEEEECCCCHHCCCC
GLDLAHTRVRLLIGPEGGLSAEEIAMTETYQFTDVLLGPRVLRTETASLTAITALQLRFG
CCEECCEEEEEEECCCCCCCHHHEEEHHHHHHHHHHHCCEEEEECCCHHEEEEEEEEECC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]