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The map label for this gene is phoB [H]

Identifier: 160873961

GI number: 160873961

Start: 984619

End: 985938

Strand: Reverse

Name: phoB [H]

Synonym: Sbal195_0839

Alternate gene names: 160873961

Gene position: 985938-984619 (Counterclockwise)

Preceding gene: 160873962

Following gene: 160873960

Centisome position: 18.44

GC content: 50.0

Gene sequence:

>1320_bases
ATGAGTTTCACCAAAGCGCCACTCTTGTTACTCGGCATCAGTTTGCTGTTATCTACGCCAGTTTGGGCAGATGAAACGGG
TCCAACGAAAGCGCCATCGCGACCGAAAAATATCGTCATCATGATAGGTGATGGTATGGGGCCGTCTTACACTAGCGCCT
ACCGCTACTACAAAGATAATCCTGACACTGAAGAAGTCGAGCAAACCGTATTCGACCGTTTACTGGTGGGCATGGCAAGC
ACCTACCCCGCGAGCGTCAGTGGTTATGTGACTGATTCAGCCGCGGCCGCGACAGCGCTAGCCACAGGGGTTAAATCTTA
TAATGGGGCGATTTCGGTCGATACCCAAAAACAACCGCTGCCGACCATACTCGAAAAAGCCAAATCCCTTGGCCTCAGCA
CAGGTGTCGCGGTAACATCGCAAATCAACCACGCTACGCCAGCGGCCTTTTTGGCCCACAACGAAAGCCGTAAAAATTAC
GACGCTCTGGCACTCAGCTATTTAGAAACCAACGCCGATGTATTTTTAGGCGGTGGTCAAAAGTATTTCCCCCCAGCGCT
GTTAGAGCAGTTCAAAGCCAAGGGCTATCAGCACATCAGCCGTTTTGAGGATCTCGCCAGCATCACCCAACCAAAAGTGT
TGGGCCTGTTTGCCGAAGTACAATTGCCTTGGGCGATTGATGAGAAAGATGCAAGAAAACTCAGCACCTTAACTCAAAAG
GCACTCAGCTTACTGTCGCAAAATGACCAAGGTTTTGTGCTGTTAGTCGAAGGGAGTCTCATCGACTGGGCGGGCCACAA
TAACGATATCGCCACTGCCATGAGTGAGATGGATGAATTCTCCAATGCCATTGAAGTGGTTGAGCAATTTGTGCGTGAAC
ATCCCGATACCCTGATGGTGATCACCGCCGACCACAATACCGGTGGCCTATCGATTGGCGTCGATGGTAACTACAACTGG
AACCCTGAAATCCTGCGTAATATCTCGGCCAGCACAGACACTTTGGCCTTGGCCACCATCACTGGTGAACAATGGCAAGC
CGATCTCGCCCGCGGTTTAGGCTTTGAGCTTAGCGAAGAAGAAGTCGCTAAATTGAACGTCGCCAGAATGCAGGGATTAG
AAACCATGGCGGTGGCGATTCGCCATCTTATTGATAAACGTACTGATACAGGTTGGACAACCGACGGCCACACAGGCACA
GACGTACAAGTATTTGCCGCAGGTCCGGCATCTGAGCTGTTTAACGGCCATCAAGATAATACCGATATCGCCAATAAGAT
TTTTAGCTTATTACCTAAACCGAAGAAACCTAAAAGCTAA

Upstream 100 bases:

>100_bases
AAGCACGCATTAACACCAAAGCATAAGAATTTACCAACGAGTTTGTTGATTGTAAGGCGCGAGCAAATCGCGAGCGCCGA
ACCACTTCTAGGGATGTGAT

Downstream 100 bases:

>100_bases
GCGTTAATCGATTCCGTTCATTAAAGGCAATCACATAAGGCAACCGCATAATGCAGTTGCCTTTTTTATTGGGTCTTTTT
ATTAGGGATTTTATTAAGTC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSFTKAPLLLLGISLLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMAS
TYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNY
DALALSYLETNADVFLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQK
ALSLLSQNDQGFVLLVEGSLIDWAGHNNDIATAMSEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYNW
NPEILRNISASTDTLALATITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGT
DVQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKS

Sequences:

>Translated_439_residues
MSFTKAPLLLLGISLLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMAS
TYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNY
DALALSYLETNADVFLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQK
ALSLLSQNDQGFVLLVEGSLIDWAGHNNDIATAMSEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYNW
NPEILRNISASTDTLALATITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGT
DVQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKS
>Mature_438_residues
SFTKAPLLLLGISLLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMAST
YPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYD
ALALSYLETNADVFLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKA
LSLLSQNDQGFVLLVEGSLIDWAGHNNDIATAMSEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYNWN
PEILRNISASTDTLALATITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTD
VQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKS

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=454, Percent_Identity=29.9559471365639, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI94721246, Length=452, Percent_Identity=28.7610619469027, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI116734717, Length=456, Percent_Identity=28.0701754385965, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI294660770, Length=452, Percent_Identity=27.8761061946903, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=28.0973451327434, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI48994877, Length=328, Percent_Identity=35.3658536585366, Blast_Score=160, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320689, Length=458, Percent_Identity=30.3493449781659, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355981, Length=442, Percent_Identity=27.8280542986425, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651554, Length=463, Percent_Identity=30.0215982721382, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19921912, Length=460, Percent_Identity=26.9565217391304, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI85725200, Length=444, Percent_Identity=26.5765765765766, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI85815833, Length=444, Percent_Identity=26.5765765765766, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24657842, Length=449, Percent_Identity=27.3942093541203, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859923, Length=293, Percent_Identity=28.6689419795222, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358067, Length=449, Percent_Identity=27.3942093541203, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355151, Length=448, Percent_Identity=25.2232142857143, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24657835, Length=448, Percent_Identity=27.0089285714286, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585213, Length=478, Percent_Identity=26.1506276150628, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24657827, Length=445, Percent_Identity=26.2921348314607, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47480; Mature: 47349

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTKAPLLLLGISLLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDN
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEECCC
PDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCH
PTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADVFLGGGQ
HHHHHHHHHCCCCCCEEHECCCCCCCCHHHEECCCCCCCHHHHHHHHEECCCCEEECCCC
KYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHHHHHHH
ALSLLSQNDQGFVLLVEGSLIDWAGHNNDIATAMSEMDEFSNAIEVVEQFVREHPDTLMV
HHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ITADHNTGGLSIGVDGNYNWNPEILRNISASTDTLALATITGEQWQADLARGLGFELSEE
EEECCCCCCEEEEECCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHH
EVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELFNGHQDN
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
TDIANKIFSLLPKPKKPKS
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SFTKAPLLLLGISLLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDN
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEECCC
PDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCH
PTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADVFLGGGQ
HHHHHHHHHCCCCCCEEHECCCCCCCCHHHEECCCCCCCHHHHHHHHEECCCCEEECCCC
KYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHHHHHHH
ALSLLSQNDQGFVLLVEGSLIDWAGHNNDIATAMSEMDEFSNAIEVVEQFVREHPDTLMV
HHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ITADHNTGGLSIGVDGNYNWNPEILRNISASTDTLALATITGEQWQADLARGLGFELSEE
EEECCCCCCEEEEECCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHH
EVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELFNGHQDN
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
TDIANKIFSLLPKPKKPKS
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]