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The map label for this gene is phoA [H]

Identifier: 160873817

GI number: 160873817

Start: 842309

End: 843805

Strand: Direct

Name: phoA [H]

Synonym: Sbal195_0695

Alternate gene names: 160873817

Gene position: 842309-843805 (Clockwise)

Preceding gene: 160873814

Following gene: 160873818

Centisome position: 15.75

GC content: 49.16

Gene sequence:

>1497_bases
ATGAATATTAAACGGATCGCTGCAGCTATCTCCTGCAGCTTAATGACGGTTGTAGCCCATGCTGCAGTCCTGCCAGCAAC
ACAAACAGACAGCCAGTGGTTTAAAGACAGTGCGGCAAACGTGGTGGCTAAAGCCCAAGTCGACACTAAAAAAACCGCTA
AAAATGTGATTTTATTTGTCGGTGACGGCATGAGTATTTCGACGCTAACGGCAGCCCGTATTCTGCAAGGGCAGGATAAA
GCTGACAACCAAGGCGGCGAAGAAAACTTCTTAAGTTTCGAACAGTTCCCCCACACGGCGTTAGTGAAAACCTATAACAC
TAACCAACAAACGCCAGATTCTGCGGGCACTATGACAGCCATGGCGACAGGCGTTAAAACCAAGGCGGGTATTATCTCTA
TTTCAGATACCAGCTTACGTGGTAACTGTTTGTCTTCAAAAGGCAATGAACTTGTTTCACTGGTCGACCTTGCTAATGCC
AAAGGATTATCAACAGGCATAGTCACTACGGCGCGTTTAACGCACGCTACGCCAGCGGCAACCTATGCCAAAACCCCTGA
GCGCGATTGGGAAGGCGATTTCAACTTACCTGCCGAAGCGGTTGCGAACGGTTGTACCGACATCGCAAGCCAATTGGTGA
TGCGCGATGAAGCCAATTCGTTAACTGTGGCATTAGGTGGCGGCCGTAGCTACTTCCTACCTACAACAGTGAAAGATGGC
GAGAACAAAGCCGGTAAACGTAAAGACGGTAAAGACTTAACCAAAGCTTGGGTCGATAATTTCAGCAAATCTGCTTACGT
GTGGGACAAAAAAGGCTTCGACGCCATTGATGCTGCCAACACAGATCATTTACTCGGCCTATTCAATCCATCACACATGG
AATACGAAGCAGACCGCGCTGATGACGTAGGTGGTGAGCCATCACTAACTGAAATGACCGCCAAATCCCTCGATATTTTG
AAGAAGAACGACAAAGGCTACTTGCTAATCGTTGAATCTGGCCGTATCGACCACGGTCACCATGCGGGCAATGCAAGCCG
TGCGCTGACCGATGCGGTTGAGTTATCTAATGCAGTGAAAGCGGCAATGGATGCAACGAGCAGCGACGATACCTTGATCA
TGGTAACAGCGGATCACAGCCATGTGTTCACTATCGCAGGCTACCCAAAACGCGGTAACCCTATCTTAGGTTTAGTGCAT
AACGTCGATGGCACGTTAGCGACAGCGAATGACAACAAGCCTTACACCACGCTGGCTTATACCAACGGCCCAGGTGCTGT
GGTGGGTGTGCGTGACGACTTAACCTCAGTCGATACGCAAGATAAAGATTTTATGCAACAAGCGCTTATCCCAATGGAAA
GTGAGACTCACGCCGGTGAAGACATCAGCTTACATGCCAGAGGTCCAGGTTCGAATCTGGTTCAGGGCGTGATTGAGCAG
AACGTGATCTTCCACATTATCAACCAAGCGCAGCAGCTTGGCGGCACTAAGTACTAA

Upstream 100 bases:

>100_bases
CATCCAAATCACGCCTATTCGTCACATAAATGCCTGAAAATCGGGTCGCCTCTACAGGGAATACCCTCATACACTCAATA
ATAAATCGGTGATGAACGTG

Downstream 100 bases:

>100_bases
TCACGGATTAGTTACTGAGTCCTCACTTTTGGAGCAAAAAATGAATAAGAAATTACTAGTAGTCGCGATTTCAGCCATGC
TGGGTTTAGCCGCATGCGGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNIKRIAAAISCSLMTVVAHAAVLPATQTDSQWFKDSAANVVAKAQVDTKKTAKNVILFVGDGMSISTLTAARILQGQDK
ADNQGGEENFLSFEQFPHTALVKTYNTNQQTPDSAGTMTAMATGVKTKAGIISISDTSLRGNCLSSKGNELVSLVDLANA
KGLSTGIVTTARLTHATPAATYAKTPERDWEGDFNLPAEAVANGCTDIASQLVMRDEANSLTVALGGGRSYFLPTTVKDG
ENKAGKRKDGKDLTKAWVDNFSKSAYVWDKKGFDAIDAANTDHLLGLFNPSHMEYEADRADDVGGEPSLTEMTAKSLDIL
KKNDKGYLLIVESGRIDHGHHAGNASRALTDAVELSNAVKAAMDATSSDDTLIMVTADHSHVFTIAGYPKRGNPILGLVH
NVDGTLATANDNKPYTTLAYTNGPGAVVGVRDDLTSVDTQDKDFMQQALIPMESETHAGEDISLHARGPGSNLVQGVIEQ
NVIFHIINQAQQLGGTKY

Sequences:

>Translated_498_residues
MNIKRIAAAISCSLMTVVAHAAVLPATQTDSQWFKDSAANVVAKAQVDTKKTAKNVILFVGDGMSISTLTAARILQGQDK
ADNQGGEENFLSFEQFPHTALVKTYNTNQQTPDSAGTMTAMATGVKTKAGIISISDTSLRGNCLSSKGNELVSLVDLANA
KGLSTGIVTTARLTHATPAATYAKTPERDWEGDFNLPAEAVANGCTDIASQLVMRDEANSLTVALGGGRSYFLPTTVKDG
ENKAGKRKDGKDLTKAWVDNFSKSAYVWDKKGFDAIDAANTDHLLGLFNPSHMEYEADRADDVGGEPSLTEMTAKSLDIL
KKNDKGYLLIVESGRIDHGHHAGNASRALTDAVELSNAVKAAMDATSSDDTLIMVTADHSHVFTIAGYPKRGNPILGLVH
NVDGTLATANDNKPYTTLAYTNGPGAVVGVRDDLTSVDTQDKDFMQQALIPMESETHAGEDISLHARGPGSNLVQGVIEQ
NVIFHIINQAQQLGGTKY
>Mature_498_residues
MNIKRIAAAISCSLMTVVAHAAVLPATQTDSQWFKDSAANVVAKAQVDTKKTAKNVILFVGDGMSISTLTAARILQGQDK
ADNQGGEENFLSFEQFPHTALVKTYNTNQQTPDSAGTMTAMATGVKTKAGIISISDTSLRGNCLSSKGNELVSLVDLANA
KGLSTGIVTTARLTHATPAATYAKTPERDWEGDFNLPAEAVANGCTDIASQLVMRDEANSLTVALGGGRSYFLPTTVKDG
ENKAGKRKDGKDLTKAWVDNFSKSAYVWDKKGFDAIDAANTDHLLGLFNPSHMEYEADRADDVGGEPSLTEMTAKSLDIL
KKNDKGYLLIVESGRIDHGHHAGNASRALTDAVELSNAVKAAMDATSSDDTLIMVTADHSHVFTIAGYPKRGNPILGLVH
NVDGTLATANDNKPYTTLAYTNGPGAVVGVRDDLTSVDTQDKDFMQQALIPMESETHAGEDISLHARGPGSNLVQGVIEQ
NVIFHIINQAQQLGGTKY

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=488, Percent_Identity=43.2377049180328, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI294660770, Length=449, Percent_Identity=44.543429844098, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI157266296, Length=482, Percent_Identity=42.1161825726141, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI157266292, Length=476, Percent_Identity=43.0672268907563, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI94721246, Length=482, Percent_Identity=42.1161825726141, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI294660772, Length=403, Percent_Identity=43.6724565756824, Blast_Score=341, Evalue=1e-93,
Organism=Escherichia coli, GI48994877, Length=476, Percent_Identity=29.2016806722689, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320689, Length=341, Percent_Identity=29.9120234604106, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21355149, Length=487, Percent_Identity=40.6570841889117, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI19921912, Length=487, Percent_Identity=40.4517453798768, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI85725200, Length=470, Percent_Identity=40.6382978723404, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI85815833, Length=470, Percent_Identity=40.6382978723404, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24585213, Length=502, Percent_Identity=38.8446215139442, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI21355981, Length=482, Percent_Identity=38.5892116182573, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21358067, Length=475, Percent_Identity=39.7894736842105, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24651554, Length=477, Percent_Identity=39.622641509434, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24657835, Length=492, Percent_Identity=39.4308943089431, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI21355151, Length=481, Percent_Identity=39.0852390852391, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24657842, Length=502, Percent_Identity=38.2470119521912, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24657827, Length=483, Percent_Identity=39.7515527950311, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24651556, Length=463, Percent_Identity=39.0928725701944, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI18859923, Length=521, Percent_Identity=35.1247600767754, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24645593, Length=288, Percent_Identity=31.25, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 52927; Mature: 52927

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKRIAAAISCSLMTVVAHAAVLPATQTDSQWFKDSAANVVAKAQVDTKKTAKNVILFV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEEECCHHHCCCEEEEEE
GDGMSISTLTAARILQGQDKADNQGGEENFLSFEQFPHTALVKTYNTNQQTPDSAGTMTA
ECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHCCCEEEEEEECCCCCCCCCCCCHHH
MATGVKTKAGIISISDTSLRGNCLSSKGNELVSLVDLANAKGLSTGIVTTARLTHATPAA
HHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEEEEECCCCCCH
TYAKTPERDWEGDFNLPAEAVANGCTDIASQLVMRDEANSLTVALGGGRSYFLPTTVKDG
HHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCC
ENKAGKRKDGKDLTKAWVDNFSKSAYVWDKKGFDAIDAANTDHLLGLFNPSHMEYEADRA
CCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
DDVGGEPSLTEMTAKSLDILKKNDKGYLLIVESGRIDHGHHAGNASRALTDAVELSNAVK
CCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAMDATSSDDTLIMVTADHSHVFTIAGYPKRGNPILGLVHNVDGTLATANDNKPYTTLAY
HHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEE
TNGPGAVVGVRDDLTSVDTQDKDFMQQALIPMESETHAGEDISLHARGPGSNLVQGVIEQ
ECCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
NVIFHIINQAQQLGGTKY
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIKRIAAAISCSLMTVVAHAAVLPATQTDSQWFKDSAANVVAKAQVDTKKTAKNVILFV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEEECCHHHCCCEEEEEE
GDGMSISTLTAARILQGQDKADNQGGEENFLSFEQFPHTALVKTYNTNQQTPDSAGTMTA
ECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHCCCEEEEEEECCCCCCCCCCCCHHH
MATGVKTKAGIISISDTSLRGNCLSSKGNELVSLVDLANAKGLSTGIVTTARLTHATPAA
HHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEEEEECCCCCCH
TYAKTPERDWEGDFNLPAEAVANGCTDIASQLVMRDEANSLTVALGGGRSYFLPTTVKDG
HHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCC
ENKAGKRKDGKDLTKAWVDNFSKSAYVWDKKGFDAIDAANTDHLLGLFNPSHMEYEADRA
CCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
DDVGGEPSLTEMTAKSLDILKKNDKGYLLIVESGRIDHGHHAGNASRALTDAVELSNAVK
CCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAMDATSSDDTLIMVTADHSHVFTIAGYPKRGNPILGLVHNVDGTLATANDNKPYTTLAY
HHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEE
TNGPGAVVGVRDDLTSVDTQDKDFMQQALIPMESETHAGEDISLHARGPGSNLVQGVIEQ
ECCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
NVIFHIINQAQQLGGTKY
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]