Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 160873802

GI number: 160873802

Start: 827398

End: 828798

Strand: Reverse

Name: pleD [H]

Synonym: Sbal195_0680

Alternate gene names: 160873802

Gene position: 828798-827398 (Counterclockwise)

Preceding gene: 160873804

Following gene: 160873798

Centisome position: 15.5

GC content: 47.47

Gene sequence:

>1401_bases
GTGCCAGACATTTATCCTTTTTTATCGCAGCGCCCCCATAAACATGCGGCGGTGATTGTCGCTCTCTTACTGCTAACATC
AGCTCTTGTGATCTCTCCTTTTATTTCAATACCTTTACCTGATATTGCCCCATTTTTACCTGTGTACGGCACCGCAGTAG
TGCTACTCGAAAGTATCACCGCGTATTTATTGCTGTCGCAGTTTGCCAGTAATAGGCAAGTTTTCGCCGGTTTTGTCGCC
AGTGCGTACTTGTTTCTTATCCCCTTAGTCACAGTTCAGCTTTTAGTGTTTCCAGGAGTCTTTGCCCCCAAGGGGTTACT
CAATGCCGGATCACAAAGTGCAGTTTGGATCTGGGTCTTCTGGCATGCATCGTTTCCAACCTTAATCCTGCTGGCTTTGA
TGGCTCAACAGAAGTTAACAACACAGCAAGTTGCGCCGATGCAACTGAAATATTGGCTGTGGGGTTTTGTATTAATTCCT
GTGTTGTGCAGTTTGGCACTGGCTTTGTTTGCAACATGGGGTAGCCATTGGTTACCTATCCTGATCGCTAAAAATAGCTA
TAAGCAGTTATTACACAGCCCTTATGCCTTACTCGTATGGGGACTAAACGCACTGGCTTTAGTCTGCATGATACGGCGCG
CCCGCAGTAACAATGTGCTCTATACATGGCTGAGTGTGGCGCTGCTGGCCAGCCTGATTGATGTCACATTAACCTTATTC
GCTGGTGCCCGCTTTAGTCTAGGTTGGTACGCCGCAAGAATTAGCAGTTCGGTCTCCTCTATGGTCTTACTCTGCGCCTT
ACTATGGGAAGTCAACAGACTGTATGTCGCAACCCAAAGGGCGAATGAGTCGCTGTACCAGCAGAGTGTCAAAGACACCT
TAACAGGCTTATTTAACCGCCGTTATCTAGAATCTCAGCTAGCAATACACATAGACCATGCGAAAAGGCATAAAGAGCCA
CTGTGCTTGTTAGTGCTCGATGTCGATCACTTTAAAGCTTTTAACGACCAGCATGGCCATTTGGCTGGGGACGACTGCCT
AGTGACCGTAGCAAAACAACTGGAGCAGAGTTTATATAGACCAGCGGATTTCATCGCCCGTTATGGTGGTGAAGAGTTTA
TAGTGGTATTGCCGCATACCGATGCGAAAGACGGCATGCAGATAGCAGAACGATTGCGGCTAAACGTCAGCGCAGAAAAG
ATTGAATATCAAGGTCAGTTACTTAGCGTCACAATCAGTATAGGTTGTGCAGTATTGCGGCACAGTGAAGAGACCGCCCA
AAGCTTACTGATTGCAGCCGATAGTGCCTTATATCAAGCTAAAGCCGCAGGCCGAAATTGCGTAAAAGTCGCGGTTGACG
AACCAGACATCACTCACCCACAGCCATGCTCATCAGCTTAG

Upstream 100 bases:

>100_bases
AGCAGGCTTATAGACCCAGCTTCACTGCGTTTACTCTGGTCAAAACGCCGATAACTTTCTATAGTGACAAGAAATAAATA
TTATAAAGCAGTGAGTGGTT

Downstream 100 bases:

>100_bases
CCATTCGATTAACCGCACTTAATTAAATGGTTGCTCTGGCATAAAAGGCAGGTGACTACCAGCGGGAATCATTTTCACAG
GTTTATTTTACCGTGGCCGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVA
SAYLFLIPLVTVQLLVFPGVFAPKGLLNAGSQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIP
VLCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEP
LCLLVLDVDHFKAFNDQHGHLAGDDCLVTVAKQLEQSLYRPADFIARYGGEEFIVVLPHTDAKDGMQIAERLRLNVSAEK
IEYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITHPQPCSSA

Sequences:

>Translated_466_residues
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVA
SAYLFLIPLVTVQLLVFPGVFAPKGLLNAGSQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIP
VLCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEP
LCLLVLDVDHFKAFNDQHGHLAGDDCLVTVAKQLEQSLYRPADFIARYGGEEFIVVLPHTDAKDGMQIAERLRLNVSAEK
IEYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITHPQPCSSA
>Mature_465_residues
PDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVAS
AYLFLIPLVTVQLLVFPGVFAPKGLLNAGSQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPV
LCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLFA
GARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEPL
CLLVLDVDHFKAFNDQHGHLAGDDCLVTVAKQLEQSLYRPADFIARYGGEEFIVVLPHTDAKDGMQIAERLRLNVSAEKI
EYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITHPQPCSSA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=229, Percent_Identity=34.9344978165939, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=37.4233128834356, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI145693134, Length=200, Percent_Identity=36, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787802, Length=266, Percent_Identity=27.8195488721804, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=38.2352941176471, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=37.7777777777778, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788085, Length=206, Percent_Identity=28.6407766990291, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=34.7560975609756, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=212, Percent_Identity=31.6037735849057, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=31.3186813186813, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=33.5227272727273, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=31.4606741573034, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=220, Percent_Identity=30.4545454545455, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=26.0355029585799, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51575; Mature: 51444

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESIT
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AYLLLSQFASNRQVFAGFVASAYLFLIPLVTVQLLVFPGVFAPKGLLNAGSQSAVWIWVF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEE
WHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPVLCSLALALFATWGSHWLPI
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
RYLESQLAIHIDHAKRHKEPLCLLVLDVDHFKAFNDQHGHLAGDDCLVTVAKQLEQSLYR
HHHHHHHHEEEEHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
PADFIARYGGEEFIVVLPHTDAKDGMQIAERLRLNVSAEKIEYQGQLLSVTISIGCAVLR
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHCCEEEEEEHHHHHHHHH
HSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITHPQPCSSA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESIT
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AYLLLSQFASNRQVFAGFVASAYLFLIPLVTVQLLVFPGVFAPKGLLNAGSQSAVWIWVF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEE
WHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPVLCSLALALFATWGSHWLPI
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
RYLESQLAIHIDHAKRHKEPLCLLVLDVDHFKAFNDQHGHLAGDDCLVTVAKQLEQSLYR
HHHHHHHHEEEEHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
PADFIARYGGEEFIVVLPHTDAKDGMQIAERLRLNVSAEKIEYQGQLLSVTISIGCAVLR
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHCCEEEEEEHHHHHHHHH
HSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITHPQPCSSA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA