Click here to switch to the map view.

The map label for this gene is ansB [H]

Identifier: 160873768

GI number: 160873768

Start: 776228

End: 777286

Strand: Direct

Name: ansB [H]

Synonym: Sbal195_0646

Alternate gene names: 160873768

Gene position: 776228-777286 (Clockwise)

Preceding gene: 160873759

Following gene: 160873769

Centisome position: 14.52

GC content: 47.97

Gene sequence:

>1059_bases
ATGAATTTTTCAATGCGTAAGCTCTTGCTCTCGGTCACCTTAGGGTTAGTTCTCTCTTCTGCGGCGCAGGCGCTTCCACA
GGTACGGATCATTGCCACGGGTGGCACTATTGCCGGAGTAGGGCAATCGGCCACTGAGTCTAACTATCAGGCTGGTGAAG
TGGGCATTAGTAGCTTAATTGCGGCCGTGCCTGAAATGAAACAGCTTGCCGATATTCAAGGTGAGCAACTGGTTAAAATC
GGTTCGCAGGACATGAATGATAAAGTTTGGCTAGCGTTAGCCAAGCGCATCAATCTGTTACTGGCCGATAAAGAGGTCGA
CGGTATCGTCATCACTCACGGCACCGACACCTTAGAAGAAACCGCTTATTTTCTAAACCTCACCATCAAGAGCGATAAAC
CTGTGGTATTGGTTGGCGCTATGCGTCCATCAACCGCCATGAGCGCCGATGGTCCAATGAATCTGTATAACGCCGTCGCA
ACCGCCGCGAACCTTGAATCTAAAGGTCGAGGTGTATTAGTGGTAATGAACGATACCGTATTGAACGCCCGCGACGTCAC
TAAGACTAATACCACAGGTGTGCATACCTTTGCCGCGCCTAACTTTGGCCCGCTTGGCGTGATCCATAACAGCAAGATTG
AGTACATGGGTAATACTGAGCGTCAACATACTACGCAAACGCCTTTTGATGTCAGCAAACTCGACAAGTTACCGCAGGTA
GGCATAGTGTATAACTACGCCAATGCCAGTGATTTACCAGCCAAAGCTATGGTTGAAGCGGGTTTTGATGGCATAGTCAG
TGCCGGTGTGGGTAACGGTAATATCTACCACTCTATATTCGATGTTTTAGCAAAAGCGAGTCAAAAAGGCACCTTAGTCG
TTCGCTCTAGCCGAGTGCCCACAGGTGCAACGACGCTCGATGCTGAGATAGATGATGCTAAATACGGTTTTATTGCAGCA
GGTAATCTTAACCCGCAAAAAGCCCGTATATTGTTGATGCTATCTTTAACGAAAAGCAAAGATCCACAGCAGATCCAACG
TTGGTTTCAACAGTTTTAG

Upstream 100 bases:

>100_bases
ACGCTAAATTTGTTATTTCATTTTAGTGAGAATTATCACGGTTAGAGTTAAATAATAGTGCACACTGTGGAGCGTTTATT
ATTAATAAAAGGATGTAAGC

Downstream 100 bases:

>100_bases
GATTTCAGTTGAGTGATAAAAAAAAGCCCTGCGCATATTCGTAGGGCTTTTGCATTTTGTCATTGAGGAGCTATCCATGA
CGTTGTTTTTCCTAGATGCC

Product: L-asparaginase

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNFSMRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQLVKI
GSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVA
TAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKLPQV
GIVYNYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYGFIAA
GNLNPQKARILLMLSLTKSKDPQQIQRWFQQF

Sequences:

>Translated_352_residues
MNFSMRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQLVKI
GSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVA
TAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKLPQV
GIVYNYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYGFIAA
GNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
>Mature_352_residues
MNFSMRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQLVKI
GSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVA
TAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKLPQV
GIVYNYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYGFIAA
GNLNPQKARILLMLSLTKSKDPQQIQRWFQQF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=352, Percent_Identity=67.8977272727273, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1788065, Length=343, Percent_Identity=25.9475218658892, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=337, Percent_Identity=24.9258160237389, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17558278, Length=337, Percent_Identity=24.9258160237389, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=337, Percent_Identity=24.9258160237389, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193207372, Length=386, Percent_Identity=23.8341968911917, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323189, Length=364, Percent_Identity=38.7362637362637, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323187, Length=364, Percent_Identity=38.7362637362637, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323186, Length=364, Percent_Identity=38.7362637362637, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323184, Length=364, Percent_Identity=38.7362637362637, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320527, Length=345, Percent_Identity=37.3913043478261, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24645530, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37563; Mature: 37563

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00445 FGGY_KINASES_2 ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSMRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLI
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHH
AAVPEMKQLADIQGEQLVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEE
HHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEECCCCCHHC
TAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVATAANLESKGRGVLVVMNDTV
CEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCE
LNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKLPQV
ECCCCCCCCCCCCEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCCCCCHHHHHCCCCC
GIVYNYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVP
EEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TGATTLDAEIDDAKYGFIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
CCCEEEECCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNFSMRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLI
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHH
AAVPEMKQLADIQGEQLVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEE
HHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEECCCCCHHC
TAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVATAANLESKGRGVLVVMNDTV
CEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCE
LNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKLPQV
ECCCCCCCCCCCCEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCCCCCHHHHHCCCCC
GIVYNYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVP
EEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TGATTLDAEIDDAKYGFIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
CCCEEEECCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]