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The map label for this gene is outD [H]

Identifier: 160873627

GI number: 160873627

Start: 577006

End: 578685

Strand: Direct

Name: outD [H]

Synonym: Sbal195_0505

Alternate gene names: 160873627

Gene position: 577006-578685 (Clockwise)

Preceding gene: 160873626

Following gene: 160873628

Centisome position: 10.79

GC content: 48.1

Gene sequence:

>1680_bases
ATGAAAGCGATTAAATACTTTACACCTCTGCTCTCACTTTGCCTTGTGGCGTGTCAGACCACAAACAGGCCACAGCCGGA
GGCGAGTAAGGCGGAATTAGCGGCCTCAATGGCGACGGCTACAGCATCAACGACGCCACCGCCTGCGACTATGCCGGATG
CGGTTCAGCGAGAATTGAATTCGGGCGCTTTACTGTCAGGTTTAACCCCTGCGCTGGAACCTGAGCGTCGTTTTGATGTC
TCTGCCCATGATGTGGATGCCAAAGTATTTTTTCCAAGCTTAGTCCAAGGTACGCCGCTGAGTGTTGCCGTGCATCCTGA
TGTGCAAGGTACGATTTCGCTATCCCTTAAAGGTGTAACCTTAAGCGAAGCGATTCAAGTGGTTGAAGATATCTACGGTT
ACGAAGTGAGCCGTGAAGGGCGTATTCTAAGAGTGTTCCCCGCTGGAATGCGTACTGAGACTTTCCCGCTGAATTATCTT
TATATGGAGCGCGACGGCTTATCAGTGACCTCTGTGAGTTCTGGACGGATTTCTGATAGCAATAATTCCAATAGTAATTC
GAGCAATAACAACTCAAATAACAGTGGTTCGAGTAATAACAGCTCCAACAGCAGTAATGGTAATAACTCCAGCAGCAATG
GCAGCAGCGATAGCAGTAACGGCACATTTATCCGCTCGCGCACTAAGACTGATTTTTGGGGCGAGCTGAAAGAAACGCTG
ACGGCGATTATTGGTGACACAGGTAATGGGCGGCAAGTGGTGGTCACACCGCAGGCGGGTTTAGTGACCATTCGTGCTTT
CCCTAGTGAATTACGCCAAGTTCGCACTTTCTTAAATTCTGCCGAGAGTCATCTGCAGCGCCAAGTAATCTTGGAGGCTA
AGATCATCGAAGTCACATTATCTGATGGCTATCAGCAAGGTATCCAATGGGAGAATGTGCTGGGTCATGTCGGCAATACG
AATGTGAATTTTGGGACTTCGAAAGGCCCTGGGTTAAGCGATAAAATCACCTCGGCGATTGGTGGCGTGACCTCGCTGAG
TATTAAAGGCTCCGACTTTACGACCATGATCAATCTGTTAGATACCCAAGGCGATGTGGATGTATTGTCTAGCCCAAGGG
TAACAGCATCGAATAACCAAAAAGCGGTGATTAAAGTCGGTACCGATGAGTATTTTGTCACTGACGTTTCATCGACCACA
GTAGCGGGCGCAACGCCTGTGACCACTCCACAAGTCGAGCTCACGCCGTTTTTCTCAGGCATTGCCTTAGATGTCACGCC
GCAAATTGATAGTGACGGTAATGTGTTATTGCACGTGCATCCATCCGTTATCGATGTGAAAGAGCAGACTAAAGATATCA
AAGTGAGTGATGCTTCGTTGGAGCTACCTTTAGCGCAGAGTGAAATTCGCGAGTCTGATACCGTTATCCGCGCCGCTTCC
GGTGACGTAGTCATCATAGGTGGCTTGATGAAGAGCGAAAATACCGAAGTCGTCTCGCAAGTGCCTTTGTTGGGGGATAT
TCCTTTCCTCGGGGAATTGTTTAAAAACCGCAGCAAGCAAAAGAAGAAAACTGAGCTGATTATTCTGTTAAAACCTACGG
TAGTTGGCGTCGATACCTGGAAAACTGAGCTACAACGTTCTAAGACCTTATTAGATCGTTGGTATCCGGAGACAAAGTAA

Upstream 100 bases:

>100_bases
TGAGTATTGTCAAGATCACCCCCACTTATGTGTCGCTGTCCGATGGACGGAAATTGACATTGTTTCAAGCAATAACAGAG
AGATAGGGAATACACCTCAG

Downstream 100 bases:

>100_bases
GCTTCTATGTATCAGAAGCATTTTGGCTTAAACCAAGCCCCATTTTCATTGACACCCAATACCGGATTTTTTTTCGGCCT
ATCGCCACACGTAGAAGCCC

Product: pilus (MSHA type) biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALEPERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNSSNSSNGNNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWENVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VAGATPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSENTEVVSQVPLLGDIPFLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK

Sequences:

>Translated_559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALEPERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNSSNSSNGNNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWENVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VAGATPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSENTEVVSQVPLLGDIPFLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK
>Mature_559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALEPERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNSSNSSNGNNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWENVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VAGATPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSENTEVVSQVPLLGDIPFLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=277, Percent_Identity=27.4368231046931, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1789793, Length=272, Percent_Identity=28.3088235294118, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 59939; Mature: 59939

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
SGALLSGLTPALEPERRFDVSAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVT
CCCEECCCCCCCCCCHHCCCCCCCCCCEEECHHHHCCCCEEEEECCCCCEEEEEEEECCC
LSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYLYMERDGLSVTSVSSGRISDS
HHHHHHHHHHHHCCEECCCCCEEEEECCCCCCCEECEEEEEEECCCCEEEEECCCCCCCC
NNSNSNSSNNNSNNSGSSNNSSNSSNGNNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTL
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
SDGYQQGIQWENVLGHVGNTNVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLL
CCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHH
DTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAGATPVTTPQVELTPFFSG
CCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCEEEEECCCC
IALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
EEEEEECCCCCCCCEEEEECCCEEECHHHCCCEEEECCEEECCCCHHHHCCCCCEEEECC
GDVVIIGGLMKSENTEVVSQVPLLGDIPFLGELFKNRSKQKKKTELIILLKPTVVGVDTW
CCEEEEECEECCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHH
KTELQRSKTLLDRWYPETK
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
SGALLSGLTPALEPERRFDVSAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVT
CCCEECCCCCCCCCCHHCCCCCCCCCCEEECHHHHCCCCEEEEECCCCCEEEEEEEECCC
LSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYLYMERDGLSVTSVSSGRISDS
HHHHHHHHHHHHCCEECCCCCEEEEECCCCCCCEECEEEEEEECCCCEEEEECCCCCCCC
NNSNSNSSNNNSNNSGSSNNSSNSSNGNNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTL
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
SDGYQQGIQWENVLGHVGNTNVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLL
CCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHH
DTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAGATPVTTPQVELTPFFSG
CCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCEEEEECCCC
IALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
EEEEEECCCCCCCCEEEEECCCEEECHHHCCCEEEECCEEECCCCHHHHCCCCCEEEECC
GDVVIIGGLMKSENTEVVSQVPLLGDIPFLGELFKNRSKQKKKTELIILLKPTVVGVDTW
CCEEEEECEECCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHH
KTELQRSKTLLDRWYPETK
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]