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The map label for this gene is priA [H]

Identifier: 160873618

GI number: 160873618

Start: 566665

End: 568860

Strand: Reverse

Name: priA [H]

Synonym: Sbal195_0496

Alternate gene names: 160873618

Gene position: 568860-566665 (Counterclockwise)

Preceding gene: 160873653

Following gene: 160873617

Centisome position: 10.64

GC content: 50.27

Gene sequence:

>2196_bases
ATGTCATTGTTTGTCGAAGTTGCGTTACCTGTGCCTATGCGGCAAACCTTTAGCTACCGCGTACCCGAAACTTATCCTAG
TACACCGCAGATAGGCGTGCGCGTTAAAGTCCCCTTCGGTCGTCAACAATTAATCGGATTAGTCACAGGTATCACTGATA
CATGTAGCTTAGCGCCTAAGCAGATTAAGTCTGTGCTTGAGTTTATCGACGATAAACCTTTGTTGCCTGAATCACTTTAT
AAATTAACCCTTTGGGCCGCGAGATACTATTTTTGCAGCCAAGGGCAGATGCTAACCCAAGCCTTACCTGTGGCCCTACG
TAAAGGGCTCGATGCCGCGCCGCAAAAGATCCAATTCTGGCAATTAACTGAGATAGGCCAAAATATTGCGCCAGAAGCGG
TTAAGCGAGCACCCGCACAGAAAAAATTACTCGAGTTACTCAAACCCGCCAGCTTAACCCAAGAAGAAGTGATCAGCTTA
GATATCAGTAAAGCGGCACTGAAAGCCCTAGAAGATAAAGGCTGGGTGGCACGCCATGAAAAAATTGCCGAGCTTAACCT
CGACTGGCGCACCACACTCGAAATGGACGAAACGCCGCATAAACTCAATAAAGAACAAGCCGTCGCCGTTGCCACTTTAA
ATCTGCAACAAGGGTATCACTGTACTTTATTAGAAGGCATTACCGGTTCGGGTAAAACGGAAGTCTATTTGGCGGTGCTG
GAAGAAATTCTTAAGCAAGGCAAGCAAGCGTTAATTTTAGTGCCTGAAATCGGTTTAACACCCCAGACAATCAATCGCTT
TAAGCGCCGTTTTAAAGTCAATGTCGCCATATTGCACTCAGGGTTAACCGACAATCAAAGACTCGAAGCTTGGCGCCAAG
CCCGCTGCGGTCAGGCCGCCATCATTATCGGCACCCGCTCGGCCTTGTTCACGCCGATGGCCTATCCCGGCATCATCATA
CTCGATGAAGAACACGACAGTAGCTTCAAGCAACAGGAAGGTGTCGGTTATCACGCCCGCGACTTGGCAGTCATGCGTGG
CCATTTAGAATCAATTCCTGTGATATTGGGCACGGCAACCCCCTCACTCGAAAGTCTACAAAATGCCTTAAGTGGCCGTT
ACCATCATTTGCAATTGGGTGAGCGCGCTGGCTCGGCTAAAAAAGTGCGCCAAGGGATTATCGACATTAAAAATATGCCG
TTAAAAGCAGGAATGTCGGTGCCGCTGCTGAACGAAATCCGTCTGCATTTAGACGCAGGCAACCAAGTATTACTGTTTCT
CAATCGCCGCGGCTTCGCACCAGCCCTGCTTTGTCACGAGTGTGGGCACTTACACGAGTGCGACCGCTGCGACGCGTTTT
TCACAGTTCATCAATCCCTTGGTGAAATTTGCTGCCACCATTGTGGCAATCAATATGCCATCCCTAAGCAATGCCACAAA
TGCGGTAGCACTATGCTGATGGGCCAAGGTATAGGCACAGAGCAACTCGCCGAGGCACTGCAACTGGAGTTTCCGAAACA
TCCAGTAGTGCGCATCGACCGCGATACCACTCGCTTGAAAGGCTCACTAGAAACCCACTTAAACGCAATTCATAAGGGCG
AATATAAAATCCTCGTCGGCACGCAAATGCTGGCTAAGGGTCATCACTTCCCTGATGTGACGCTTGTTGGGCTACTCGAT
GTCGATGGCGCCTTATTTAGCGCCGACTTTCGCGCGCCCGAGCGTTTTGGGCAGCTCTATACCCAAGTCGCCGGTCGTGC
GGGCCGTGCCAGTAAACCCGGCACGGTATTATTGCAAACTCACCAGAGCGACAATCCCATTTTGCGGGATTTACTGCACC
GCGGTTATGGCGAGTTTGCTCGCAGCCAGTTAAAAGAACGTCAACAAGCACTGCTGCCGCCAGTATGGCACATGGTGTTG
GTGCGCGCCGAAGCCCATTTAGCCAGTGATGCCGATAACTTTCTCAACGCCTTCGCCGCGCTTTTGCCGCAAGATAAAGA
ATTTGAGGTAATTGGTCCTATGCCAGCGCCCTTAGATAAAAAAGCCGGCAAGTTCCGTCGCCAACTGCTATTCCAAGCTA
AAAACCGCAACCGTCTGCAGCAAGAGTTTGAACGTATTCATCCGCTTGTAGAGCAGCTTATCGAAGCCAAGCGCTGCCGA
TGGAGCTTAGACAGAGATCCGCAGGATCTCCTCTGA

Upstream 100 bases:

>100_bases
ATTTACTCTCAACTATCCTTGGTAAACCATCATTAGGGCTAGCCCTAGCCAGCCGCGCTGAGTACACTAAACCACATTAT
TGCCTAAGACTTGCCTGCAT

Downstream 100 bases:

>100_bases
GTAGCGTATTTGATAAGTATTTAAGCGCCATTTGATAAAAAATCATCGGGAAGATAGCAATTGTTAACCACAAACGGCTA
AAATATGCGGTTATTCCCTG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSLFVEVALPVPMRQTFSYRVPETYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLY
KLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISL
DISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLGERAGSAKKVRQGIIDIKNMP
LKAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPVWHMVL
VRAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
WSLDRDPQDLL

Sequences:

>Translated_731_residues
MSLFVEVALPVPMRQTFSYRVPETYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLY
KLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISL
DISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLGERAGSAKKVRQGIIDIKNMP
LKAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPVWHMVL
VRAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
WSLDRDPQDLL
>Mature_730_residues
SLFVEVALPVPMRQTFSYRVPETYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLYK
LTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLD
ISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVLE
EILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIIIL
DEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLGERAGSAKKVRQGIIDIKNMPL
KAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHKC
GSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLDV
DGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPVWHMVLV
RAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCRW
SLDRDPQDLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=50.343878954608, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81772; Mature: 81641

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFVEVALPVPMRQTFSYRVPETYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPK
CCEEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCHH
QIKSVLEFIDDKPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
QLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLDISKAALKALEDKGWVARHE
EHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
HHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMAYPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTAT
EEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCC
PSLESLQNALSGRYHHLQLGERAGSAKKVRQGIIDIKNMPLKAGMSVPLLNEIRLHLDAG
CCHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHCCCCHHCCCCCCCHHHEEEEEECC
NQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHCCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
HQSDNPILRDLLHRGYGEFARSQLKERQQALLPPVWHMVLVRAEAHLASDADNFLNAFAA
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSLDRDPQDLL
CCCCCCHHHCC
>Mature Secondary Structure 
SLFVEVALPVPMRQTFSYRVPETYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPK
CEEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCHH
QIKSVLEFIDDKPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
QLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLDISKAALKALEDKGWVARHE
EHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
HHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMAYPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTAT
EEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCC
PSLESLQNALSGRYHHLQLGERAGSAKKVRQGIIDIKNMPLKAGMSVPLLNEIRLHLDAG
CCHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHCCCCHHCCCCCCCHHHEEEEEECC
NQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHCCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
HQSDNPILRDLLHRGYGEFARSQLKERQQALLPPVWHMVLVRAEAHLASDADNFLNAFAA
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSLDRDPQDLL
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]