Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 160873594

GI number: 160873594

Start: 540866

End: 541777

Strand: Reverse

Name: dmlR [H]

Synonym: Sbal195_0472

Alternate gene names: 160873594

Gene position: 541777-540866 (Counterclockwise)

Preceding gene: 160873598

Following gene: 160873593

Centisome position: 10.13

GC content: 52.41

Gene sequence:

>912_bases
ATGTATCATTTAAACGATCTGCAATTAGCGCTGCGTATCGCAGCCTTAGAGAGTCTCTCGGCAGCCAGTAAAGAAATAGG
CATGACGCCGGCAGCGGCAAGTGCCGCGCTGCAAAGATTAGAGAAAAAGCTAGGATGTCAGCTATTTGCTCGCTCGACGC
GGCGCTTACAGCTCACAGAAGAAGGGCGGATCTTTCTCGATTCGGCGAAACCCGCACTGGCGATTCTCGAAACCGCCAGC
GCCAATTTAGCCGAACACAGGGGTGAGCTTAAGGGCGAAGTACGCCTCGCCTTACCTTCGGATATTGGCCGCAGCCAGAT
CCGTGCTTGGCTCGATGAATTGATGGACAGCGCCCCAGAACTGACAGTGCAGCTGTATTTTGGCGATCATATGACAGACT
TAATCGACCAAAATATCCATTTGGCTTTGCGCTATGGCCAACTTGACGACTCAAACCTTAAAAGGCGTCAGCTCGCCATG
ACCCCAAGGGTACTGGTCGCCTCACCCGCTTATCTGGCACAACACGGCACACCAAGCACGCTAAATGAACTGAGCCAACA
CGCAGTACTGATCTTAAATCGCGGCGGTGAGCCGTGGCAGAAATGGAAGTTAAGTCATAACGGCGCCCCCGTTGAAATCG
AAGTCAGGGGCCAACGTTTCTGTAACGATGGCGCAGTGATCCGCGACTGGGCACTCTCAGGTCGTGGGATTGCCTATAAG
AGTTGGCTCGATGTGGCGCAAGATGTGACCGAAGGCAGACTCCACTTAGTGCTAGCCGATAAAATAGCCCCGCCGATCCC
ACTGCAATTGGTATACCTACAAACCGATTATCCCAGCCATAAGATCCGTCGCACCATAGAGTTTTTAGTAGAAAAGCTTG
GTCAGTTCGCCCAAAACTATCCACTGCCCTAA

Upstream 100 bases:

>100_bases
ACCAGAAATTAAGATGAAACATCAGTGGTGCAATTGTGCATTAGTTAATCTATATTGATAATCAGGCTATTATTAAAATC
ATTTTCAAAAATAATTTGAT

Downstream 100 bases:

>100_bases
ATTGCAAACGTCCAAAGTATCCGAATAGAAAACCACGATAAACAAGTGGTCATGTGAAGAAAATAACCACCATCAAGTCG
TTGCTAGATGATAGAAAACT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MYHLNDLQLALRIAALESLSAASKEIGMTPAAASAALQRLEKKLGCQLFARSTRRLQLTEEGRIFLDSAKPALAILETAS
ANLAEHRGELKGEVRLALPSDIGRSQIRAWLDELMDSAPELTVQLYFGDHMTDLIDQNIHLALRYGQLDDSNLKRRQLAM
TPRVLVASPAYLAQHGTPSTLNELSQHAVLILNRGGEPWQKWKLSHNGAPVEIEVRGQRFCNDGAVIRDWALSGRGIAYK
SWLDVAQDVTEGRLHLVLADKIAPPIPLQLVYLQTDYPSHKIRRTIEFLVEKLGQFAQNYPLP

Sequences:

>Translated_303_residues
MYHLNDLQLALRIAALESLSAASKEIGMTPAAASAALQRLEKKLGCQLFARSTRRLQLTEEGRIFLDSAKPALAILETAS
ANLAEHRGELKGEVRLALPSDIGRSQIRAWLDELMDSAPELTVQLYFGDHMTDLIDQNIHLALRYGQLDDSNLKRRQLAM
TPRVLVASPAYLAQHGTPSTLNELSQHAVLILNRGGEPWQKWKLSHNGAPVEIEVRGQRFCNDGAVIRDWALSGRGIAYK
SWLDVAQDVTEGRLHLVLADKIAPPIPLQLVYLQTDYPSHKIRRTIEFLVEKLGQFAQNYPLP
>Mature_303_residues
MYHLNDLQLALRIAALESLSAASKEIGMTPAAASAALQRLEKKLGCQLFARSTRRLQLTEEGRIFLDSAKPALAILETAS
ANLAEHRGELKGEVRLALPSDIGRSQIRAWLDELMDSAPELTVQLYFGDHMTDLIDQNIHLALRYGQLDDSNLKRRQLAM
TPRVLVASPAYLAQHGTPSTLNELSQHAVLILNRGGEPWQKWKLSHNGAPVEIEVRGQRFCNDGAVIRDWALSGRGIAYK
SWLDVAQDVTEGRLHLVLADKIAPPIPLQLVYLQTDYPSHKIRRTIEFLVEKLGQFAQNYPLP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=32.5503355704698, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=30.4498269896194, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=30.6666666666667, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=26.3698630136986, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=26.6903914590747, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=27.5167785234899, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787128, Length=301, Percent_Identity=24.2524916943522, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788706, Length=193, Percent_Identity=25.9067357512953, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=24.4186046511628, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=26.7123287671233, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33806; Mature: 33806

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHLNDLQLALRIAALESLSAASKEIGMTPAAASAALQRLEKKLGCQLFARSTRRLQLTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHCCCCEEEEEC
EGRIFLDSAKPALAILETASANLAEHRGELKGEVRLALPSDIGRSQIRAWLDELMDSAPE
CCCEEEECCCCCEEHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
LTVQLYFGDHMTDLIDQNIHLALRYGQLDDSNLKRRQLAMTPRVLVASPAYLAQHGTPST
EEEEEEECCHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEECCHHHHCCCCCHH
LNELSQHAVLILNRGGEPWQKWKLSHNGAPVEIEVRGQRFCNDGAVIRDWALSGRGIAYK
HHHHHHCEEEEEECCCCHHHHEEECCCCCCEEEEECCCEECCCCCEEEEEECCCCCCCHH
SWLDVAQDVTEGRLHLVLADKIAPPIPLQLVYLQTDYPSHKIRRTIEFLVEKLGQFAQNY
HHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
PLP
CCC
>Mature Secondary Structure
MYHLNDLQLALRIAALESLSAASKEIGMTPAAASAALQRLEKKLGCQLFARSTRRLQLTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHCCCCEEEEEC
EGRIFLDSAKPALAILETASANLAEHRGELKGEVRLALPSDIGRSQIRAWLDELMDSAPE
CCCEEEECCCCCEEHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
LTVQLYFGDHMTDLIDQNIHLALRYGQLDDSNLKRRQLAMTPRVLVASPAYLAQHGTPST
EEEEEEECCHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEECCHHHHCCCCCHH
LNELSQHAVLILNRGGEPWQKWKLSHNGAPVEIEVRGQRFCNDGAVIRDWALSGRGIAYK
HHHHHHCEEEEEECCCCHHHHEEECCCCCCEEEEECCCEECCCCCEEEEEECCCCCCCHH
SWLDVAQDVTEGRLHLVLADKIAPPIPLQLVYLQTDYPSHKIRRTIEFLVEKLGQFAQNY
HHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
PLP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]