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The map label for this gene is fixJ [H]

Identifier: 160873591

GI number: 160873591

Start: 535298

End: 535900

Strand: Reverse

Name: fixJ [H]

Synonym: Sbal195_0467

Alternate gene names: 160873591

Gene position: 535900-535298 (Counterclockwise)

Preceding gene: 160873593

Following gene: 160873590

Centisome position: 10.02

GC content: 48.76

Gene sequence:

>603_bases
ATGTCTATAAAACTGCCGCTGTATTTAGTTGATGACGATGAAGCGATTCTGGATTCGCTGGGTTTTATGCTTGGGCAATT
TGGCTACCAAGTGCAAACCTTCAACAGCGGCCGCGACTTCCTTGCGCACTGCGCATTATCGCAGGCAGGCTGCGTGATTT
TAGATAGCCGAATGCCAGAGATCACCGGCCAAGAAGTACAGCAAAAACTGCTCGAAACCCACAGTCCGCTTGGCGTCATC
TTTCTCACTGGCCACGGCGACTTACCTATGGCGCTGAGCGCCTTTCGTAAGGGAGCCTGCGACTTTTTTCAAAAACCTGT
GTCCGGCAAGGCATTAGTACAAGCGATAGAAAAAGCACAGCGTGAAAGCCAGGCGGCCTTCGAGCAGCAAAGTCTGCAAC
ATAAGTTTGACCAACTGACCGAGCGCGAACAGCAAGTGTTAGCCCATGTGATCCAAGGCATGACCAATAAGCAAATCTCG
GAGGCGATGTTTTTATCCCTGCGCACAATTGAAGTACACCGCGCAAAAATCATGAAAAAACTCGAAGTGAATAATATGGC
AGAGTTAGTGCAGCACTTAGGGAATCTACATTCAGCCTTATAA

Upstream 100 bases:

>100_bases
CTAATTTAGTGATGAACCTCCCAAGACCTAGGCTTAAAATTGAGCCTGACACAGACGCGGCTTAAGCTGATATAGCTCTC
CCGCCTCCACAAGGAAATCC

Downstream 100 bases:

>100_bases
GTCAGTGACCCATAGTCGCTAAACAACCGCGACGCCTTCTCGCCACGCATAAGGATTTAACCGTTAAACCCTTTTTACCC
AGACCATTCAAGATACCGTA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSIKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLLETHSPLGVI
FLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQAAFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQIS
EAMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGNLHSAL

Sequences:

>Translated_200_residues
MSIKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLLETHSPLGVI
FLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQAAFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQIS
EAMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGNLHSAL
>Mature_199_residues
SIKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLLETHSPLGVIF
LTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQAAFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISE
AMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGNLHSAL

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790102, Length=168, Percent_Identity=31.547619047619, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=32.7433628318584, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788905, Length=124, Percent_Identity=37.0967741935484, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=32.1739130434783, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22309; Mature: 22178

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPE
CCEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCC
ITGQEVQQKLLETHSPLGVIFLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQ
CCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RESQAAFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISEAMFLSLRTIEVHRAKIMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVNNMAELVQHLGNLHSAL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPE
CEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCC
ITGQEVQQKLLETHSPLGVIFLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQ
CCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RESQAAFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISEAMFLSLRTIEVHRAKIMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVNNMAELVQHLGNLHSAL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]