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The map label for this gene is icaA [H]

Identifier: 160873572

GI number: 160873572

Start: 514903

End: 516234

Strand: Direct

Name: icaA [H]

Synonym: Sbal195_0448

Alternate gene names: 160873572

Gene position: 514903-516234 (Clockwise)

Preceding gene: 160873571

Following gene: 160873573

Centisome position: 9.63

GC content: 43.09

Gene sequence:

>1332_bases
ATGATGAGTGAATTCAATACCATCACTTATATGTTGCTGGATATCTGGCATCAACTTGCACAGCACTTGACGTTCTTTGT
GTGGTTGGTGCCGTTGATATTATTGTATGAACTCCCCTTAATGCTACTCGTAGTGACAGGCATTTTACGTTTCTTTTATA
CCAGCTTTATGCGAGTGCCTAAGCAGTCGCTGTTTAGACCTAAAGTGTCCTGTGTGATTACCTGCTATGCCGAAGGTGAG
GCTGTGAAGTCAACGATTGATTCTTTTATCGAGCAAGTTTATGACGGTGAGATAGAGATCATTGCTGTGGTTGATGGGGC
TGTTCAAAATGGATTGACTTACCGTGTTGCTATGGCCGCGGCTAAGGCGTGCCAAGCTCATAATCGAACTGTGATTGTGT
TGCCTAAATGGCAGCGGGGTGGACGAGTCTCCACATTAAATGCGGGATTGGCGACGGCGACGGGTGAGATAGTGATCAAT
GCCGATGCTGATACTTCCTTTGATAACGATATGGTGTCGCAGATAGTGCCACATTTTGAAGACCCGAATGTGCCCGCTGT
GGGTGGAGCATTGCGAGTGAGGAATGTGAGTGAATCCATATTTACTCGGATGCAAGCTATTGAATACTTGATCTCAATGC
AAGGTGGTAAAACAGGGTTAGGGCAGTGGAATTTGCTGAACAATATTTCTGGTGCTTTTGGCGCTTTCAGACGGACCTTT
TTAATCCAAATTGGTGGCTGGGACACTCACACTGCAGAAGATCTTGATTTAACCATAAGAATAAAACAGTACTTTAAACG
ACATCCTGATTGGCGGATCCCTTTTGCTACACTGGCTATTGGTCATACGGACGCACCTGCGGATCTAAAAACCTTAGTCT
GGCAAAGGCTACGCTGGGATGGCGATTTATTGTTTTTATATTTTCGTAAACACTGGCCAGCATTTACACCCAAGCTATTG
GGTAGCGGTACTTTTGTATTTACCTTGCTCTATGGTTTTTTACAGAACGTATTAATGCCCTTTGTGATCGTTTTCTATGG
GATTGGTATTGTTGTTAGTTTCCCATGGCAGTTTATTTTGTCTATTGCATTAACGATTTATGGCTTCTATTTGGTCATTT
TAGCTTTCTTTTATTCAATGGTTCTACTGGCGATATCTGAGCGAATTCCTCAAGATTTACGCTTAGCTGTTTGGCTACCG
CTATATCCTTTTTATGCATTGTTTATGCGAATGGTGTGTCTGTTTGCCTTGTTAAACGAAATGGTGCGACGTTCACATGA
AGAAAGTTCAATGGCGCCTTGGTGGGTGTTAAAACGCGGGAGAAAGTTTTAA

Upstream 100 bases:

>100_bases
AAATCGAGTTAAGCGTTAAGGCCATTAGCCTACCAGCCAATATTGGTGAAAATGTCGGTGAGTGGCTTACAGATCAACTC
CTACAAGCTAAGCCAATGTG

Downstream 100 bases:

>100_bases
TGAAAGTGAGTTATCAAGCCAGTGACAAGGCGCTAAATCCGACTGAAGATAAGGGTATTGGAGTGCGCTATGCTGCCGCT
AAGCGTGGTGGATTCAAGGG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MMSEFNTITYMLLDIWHQLAQHLTFFVWLVPLILLYELPLMLLVVTGILRFFYTSFMRVPKQSLFRPKVSCVITCYAEGE
AVKSTIDSFIEQVYDGEIEIIAVVDGAVQNGLTYRVAMAAAKACQAHNRTVIVLPKWQRGGRVSTLNAGLATATGEIVIN
ADADTSFDNDMVSQIVPHFEDPNVPAVGGALRVRNVSESIFTRMQAIEYLISMQGGKTGLGQWNLLNNISGAFGAFRRTF
LIQIGGWDTHTAEDLDLTIRIKQYFKRHPDWRIPFATLAIGHTDAPADLKTLVWQRLRWDGDLLFLYFRKHWPAFTPKLL
GSGTFVFTLLYGFLQNVLMPFVIVFYGIGIVVSFPWQFILSIALTIYGFYLVILAFFYSMVLLAISERIPQDLRLAVWLP
LYPFYALFMRMVCLFALLNEMVRRSHEESSMAPWWVLKRGRKF

Sequences:

>Translated_443_residues
MMSEFNTITYMLLDIWHQLAQHLTFFVWLVPLILLYELPLMLLVVTGILRFFYTSFMRVPKQSLFRPKVSCVITCYAEGE
AVKSTIDSFIEQVYDGEIEIIAVVDGAVQNGLTYRVAMAAAKACQAHNRTVIVLPKWQRGGRVSTLNAGLATATGEIVIN
ADADTSFDNDMVSQIVPHFEDPNVPAVGGALRVRNVSESIFTRMQAIEYLISMQGGKTGLGQWNLLNNISGAFGAFRRTF
LIQIGGWDTHTAEDLDLTIRIKQYFKRHPDWRIPFATLAIGHTDAPADLKTLVWQRLRWDGDLLFLYFRKHWPAFTPKLL
GSGTFVFTLLYGFLQNVLMPFVIVFYGIGIVVSFPWQFILSIALTIYGFYLVILAFFYSMVLLAISERIPQDLRLAVWLP
LYPFYALFMRMVCLFALLNEMVRRSHEESSMAPWWVLKRGRKF
>Mature_443_residues
MMSEFNTITYMLLDIWHQLAQHLTFFVWLVPLILLYELPLMLLVVTGILRFFYTSFMRVPKQSLFRPKVSCVITCYAEGE
AVKSTIDSFIEQVYDGEIEIIAVVDGAVQNGLTYRVAMAAAKACQAHNRTVIVLPKWQRGGRVSTLNAGLATATGEIVIN
ADADTSFDNDMVSQIVPHFEDPNVPAVGGALRVRNVSESIFTRMQAIEYLISMQGGKTGLGQWNLLNNISGAFGAFRRTF
LIQIGGWDTHTAEDLDLTIRIKQYFKRHPDWRIPFATLAIGHTDAPADLKTLVWQRLRWDGDLLFLYFRKHWPAFTPKLL
GSGTFVFTLLYGFLQNVLMPFVIVFYGIGIVVSFPWQFILSIALTIYGFYLVILAFFYSMVLLAISERIPQDLRLAVWLP
LYPFYALFMRMVCLFALLNEMVRRSHEESSMAPWWVLKRGRKF

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=281, Percent_Identity=31.6725978647687, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50622; Mature: 50622

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSEFNTITYMLLDIWHQLAQHLTFFVWLVPLILLYELPLMLLVVTGILRFFYTSFMRVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQSLFRPKVSCVITCYAEGEAVKSTIDSFIEQVYDGEIEIIAVVDGAVQNGLTYRVAMAA
HHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHHHH
AKACQAHNRTVIVLPKWQRGGRVSTLNAGLATATGEIVINADADTSFDNDMVSQIVPHFE
HHHHHHCCCEEEEEECCCCCCEEEEECCCCEECCCCEEEECCCCCCCCHHHHHHHCCCCC
DPNVPAVGGALRVRNVSESIFTRMQAIEYLISMQGGKTGLGQWNLLNNISGAFGAFRRTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHE
LIQIGGWDTHTAEDLDLTIRIKQYFKRHPDWRIPFATLAIGHTDAPADLKTLVWQRLRWD
EEEECCCCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHHHCCC
GDLLFLYFRKHWPAFTPKLLGSGTFVFTLLYGFLQNVLMPFVIVFYGIGIVVSFPWQFIL
CCEEEEEEHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIALTIYGFYLVILAFFYSMVLLAISERIPQDLRLAVWLPLYPFYALFMRMVCLFALLNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVRRSHEESSMAPWWVLKRGRKF
HHHHCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MMSEFNTITYMLLDIWHQLAQHLTFFVWLVPLILLYELPLMLLVVTGILRFFYTSFMRVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQSLFRPKVSCVITCYAEGEAVKSTIDSFIEQVYDGEIEIIAVVDGAVQNGLTYRVAMAA
HHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHHHH
AKACQAHNRTVIVLPKWQRGGRVSTLNAGLATATGEIVINADADTSFDNDMVSQIVPHFE
HHHHHHCCCEEEEEECCCCCCEEEEECCCCEECCCCEEEECCCCCCCCHHHHHHHCCCCC
DPNVPAVGGALRVRNVSESIFTRMQAIEYLISMQGGKTGLGQWNLLNNISGAFGAFRRTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHE
LIQIGGWDTHTAEDLDLTIRIKQYFKRHPDWRIPFATLAIGHTDAPADLKTLVWQRLRWD
EEEECCCCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHHHCCC
GDLLFLYFRKHWPAFTPKLLGSGTFVFTLLYGFLQNVLMPFVIVFYGIGIVVSFPWQFIL
CCEEEEEEHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIALTIYGFYLVILAFFYSMVLLAISERIPQDLRLAVWLPLYPFYALFMRMVCLFALLNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVRRSHEESSMAPWWVLKRGRKF
HHHHCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]