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The map label for this gene is murD [H]

Identifier: 160873536

GI number: 160873536

Start: 468050

End: 469369

Strand: Direct

Name: murD [H]

Synonym: Sbal195_0411

Alternate gene names: 160873536

Gene position: 468050-469369 (Clockwise)

Preceding gene: 160873535

Following gene: 160873537

Centisome position: 8.75

GC content: 50.0

Gene sequence:

>1320_bases
ATGCAAAATCAGTATTCACACATAGTGTTAGGTTTAGGGGCAACGGGTTTGTCCGTCGTGCGCTATTTGTGTGGAAAGGG
CATTACGCCATTGGTCATGGACAGTCGTCGGCAGCCTCCGGGCGCCGAAACATTAGCGACGTCTTTTCCTGATGTGAAGC
TGATTTCTGGCGGATTCGATTGCCGTTATCTCGTGCAAGCGACGCAGATCATTATCAGTCCCGGCATTGCCACTTATACC
CCAGAGGTGCGTGCCGCTGTGGATATGGGCATCGAAGTGATTGGCGATGTGGAGCTATTTGCTCGTGAGATTGCTGACCG
TAAACCTTGCGTTATCGGTATTACTGGCTCTAACGGTAAATCAACTGTCACTACGCTTGTAGGCGAAATGGCAAGGGAAG
CGGGTATTGCCGTTGCCGTTGGTGGCAATATCGGTGTGCCTGCGCTGGATTTACTGCATGAAAATGCCGACTTATTTGTG
CTTGAGTTGTCGAGCTTTCAATTGGAAACCACCCATAGCCTTAAGTGTGTGGTGGCGACCTGTTTGAATATCAGCGAAGA
CCACATGGACAGATACAGCGATATCGCAGCCTACCGCGAAGCTAAATTACGTTTATACAAACAAAGCCGCGCCGTGCTGT
TTAATCGCGACGATAAACTGACCGTACCGCCAGAGCCAATGAACCAAAACAGTTTTGGTTTAGCTGCGCCAGAAGCGGAC
GAATGGGGTATCAGCGACAGCAAGATTTATCACGGCAGCAGCGAAATCATGCCGATCACTGAAGTCTCCCTGATTGGTAG
CCATAACCATGCCAACCTACTCGCAGCAATGGCGCTTGTGGATGTTGTCGGTGTTGATAAGCAGGTTATGGCGAAAGTGG
CGCGTAGCTTTACTGGCTTATCTCATCGTTGTGAAGTGGTTGGGATTAAGAATGGCGTGACCTATGTCAATGACTCGAAA
GCGACGAACGTCGGCGCCACCGTTGCCGCGCTCGATGGTTTGAGTGATCACTTAGGCGATATTATTTTGATTGCTGGCGG
CGACGGTAAAGGTGCTGATTTTAAACCTTTAGGCGTGGCATTAGCGAAAGTAACACACCTCATTACCTTAGGTCGCGATG
GCAAAAAGATTGCCGCTTTAAAAGACGGCGCGGTCAGAGTCGAGACTATGGCGGCTGCGGTGGCTAAAGCCGCTGAATTA
GCGACCTCTGGCGATATCGTTTTATTGTCGCCAGCCTGCGCCAGTTTAGATATGTACAGTAACTTTATGGCTCGAGGTGA
CGATTTTAGAAACCTTGTGGAGCAACTCAATGGCGAGTGA

Upstream 100 bases:

>100_bases
AAAAGGCTGGCCTGAACCTCGAGTGATAGTGCGTTTTTGGATTATCTCGATATTCCTCGTCTTACTTGGCTTAGCCACGT
TGAAGTTAAGGTAATTGGTT

Downstream 100 bases:

>100_bases
TGTAAGACAACTCAGCCTATTTGGGCGTTTGCGTTCAGCAATGCCCAATTGGCAACGTGATACCGAAGTGCCGGGCGTGC
AATTGTACGACCGCGCTTTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MQNQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGAETLATSFPDVKLISGGFDCRYLVQATQIIISPGIATYT
PEVRAAVDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMAREAGIAVAVGGNIGVPALDLLHENADLFV
LELSSFQLETTHSLKCVVATCLNISEDHMDRYSDIAAYREAKLRLYKQSRAVLFNRDDKLTVPPEPMNQNSFGLAAPEAD
EWGISDSKIYHGSSEIMPITEVSLIGSHNHANLLAAMALVDVVGVDKQVMAKVARSFTGLSHRCEVVGIKNGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFKPLGVALAKVTHLITLGRDGKKIAALKDGAVRVETMAAAVAKAAEL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVEQLNGE

Sequences:

>Translated_439_residues
MQNQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGAETLATSFPDVKLISGGFDCRYLVQATQIIISPGIATYT
PEVRAAVDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMAREAGIAVAVGGNIGVPALDLLHENADLFV
LELSSFQLETTHSLKCVVATCLNISEDHMDRYSDIAAYREAKLRLYKQSRAVLFNRDDKLTVPPEPMNQNSFGLAAPEAD
EWGISDSKIYHGSSEIMPITEVSLIGSHNHANLLAAMALVDVVGVDKQVMAKVARSFTGLSHRCEVVGIKNGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFKPLGVALAKVTHLITLGRDGKKIAALKDGAVRVETMAAAVAKAAEL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVEQLNGE
>Mature_439_residues
MQNQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGAETLATSFPDVKLISGGFDCRYLVQATQIIISPGIATYT
PEVRAAVDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMAREAGIAVAVGGNIGVPALDLLHENADLFV
LELSSFQLETTHSLKCVVATCLNISEDHMDRYSDIAAYREAKLRLYKQSRAVLFNRDDKLTVPPEPMNQNSFGLAAPEAD
EWGISDSKIYHGSSEIMPITEVSLIGSHNHANLLAAMALVDVVGVDKQVMAKVARSFTGLSHRCEVVGIKNGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFKPLGVALAKVTHLITLGRDGKKIAALKDGAVRVETMAAAVAKAAEL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVEQLNGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=444, Percent_Identity=47.2972972972973, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46453; Mature: 46453

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGAETLATSFPDVKLISGGFD
CCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCCCEEEEECCCC
CRYLVQATQIIISPGIATYTPEVRAAVDMGIEVIGDVELFAREIADRKPCVIGITGSNGK
HHHHHHHHHHEECCCCCCCCCHHHHHHHCCHHEECHHHHHHHHHCCCCCEEEEEECCCCC
STVTTLVGEMAREAGIAVAVGGNIGVPALDLLHENADLFVLELSSFQLETTHSLKCVVAT
HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCCEEEEEECCEEEECCCCHHHHHHH
CLNISEDHMDRYSDIAAYREAKLRLYKQSRAVLFNRDDKLTVPPEPMNQNSFGLAAPEAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCC
EWGISDSKIYHGSSEIMPITEVSLIGSHNHANLLAAMALVDVVGVDKQVMAKVARSFTGL
CCCCCCCEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SHRCEVVGIKNGVTYVNDSKATNVGATVAALDGLSDHLGDIILIAGGDGKGADFKPLGVA
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHCCCHHCCCCEEEEECCCCCCCCCCHHHHH
LAKVTHLITLGRDGKKIAALKDGAVRVETMAAAVAKAAELATSGDIVLLSPACASLDMYS
HHHHHHHHEECCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
NFMARGDDFRNLVEQLNGE
HHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQNQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGAETLATSFPDVKLISGGFD
CCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCCCEEEEECCCC
CRYLVQATQIIISPGIATYTPEVRAAVDMGIEVIGDVELFAREIADRKPCVIGITGSNGK
HHHHHHHHHHEECCCCCCCCCHHHHHHHCCHHEECHHHHHHHHHCCCCCEEEEEECCCCC
STVTTLVGEMAREAGIAVAVGGNIGVPALDLLHENADLFVLELSSFQLETTHSLKCVVAT
HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCCEEEEEECCEEEECCCCHHHHHHH
CLNISEDHMDRYSDIAAYREAKLRLYKQSRAVLFNRDDKLTVPPEPMNQNSFGLAAPEAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCC
EWGISDSKIYHGSSEIMPITEVSLIGSHNHANLLAAMALVDVVGVDKQVMAKVARSFTGL
CCCCCCCEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SHRCEVVGIKNGVTYVNDSKATNVGATVAALDGLSDHLGDIILIAGGDGKGADFKPLGVA
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHCCCHHCCCCEEEEECCCCCCCCCCHHHHH
LAKVTHLITLGRDGKKIAALKDGAVRVETMAAAVAKAAELATSGDIVLLSPACASLDMYS
HHHHHHHHEECCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
NFMARGDDFRNLVEQLNGE
HHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]