Click here to switch to the map view.

The map label for this gene is rstB [H]

Identifier: 160873433

GI number: 160873433

Start: 349604

End: 351211

Strand: Direct

Name: rstB [H]

Synonym: Sbal195_0308

Alternate gene names: 160873433

Gene position: 349604-351211 (Clockwise)

Preceding gene: 160873431

Following gene: 160873434

Centisome position: 6.54

GC content: 43.22

Gene sequence:

>1608_bases
ATGAAAAAATCATTATTATCTTTGGCTATTATCGTTATTTTGTCAGTGGTGGGTATTCAGCTATTTATCATTCCGCTGTT
TGAAAATAAAGTCAGGCATCCGACCGAGAAGTGGGCGCTGGAGTTGTTGACATCAGGTACGGCCAATATGCTTGCAGTGC
AATTACAGCGGTTGCCGAAGCAAGACAGGGCTGCCTATTTACAGAATATGCAGGCTAATTTTGGCTATGAACTTGCAGTG
CAAGACTATCAGCGGGATGACTTTTCAATTGTGCAGCAGCAATGGATTGATCAGAATAACGTCGTTGGCGAGCCGTTAAC
GGATTTAGTCTACCGAAGAATCGACGCCGATCAGCTGTTGGTGGTAATCAAACCTAATACCGTACCTAAATATTTAGTCA
ATGATGCTCAGCGTTGGAGTATGGGCGTATTTTATCTTATTACTCATGCGTTAAATAAGCAGCCAGAAAATAACTGGCAC
TCGATAATTGCTGTCATCAATGAGGATTTTGCCTACCCAGTGACATTGCAGTCACTTGGCGAGATACACGAAAATGATGA
GCAGATGAGTGACTTATATGCAGGACGCATTATTGCGCTGTCTACTGCGGACTCTGATGCGATTGGCTACCCAGCAGACA
GGGTGATGCAACGTATTGGCAGCAGCGACAAGGTGATTGTTCTAGGACCATTCAGCACTGAGTTAAAGCCTAAGGTGCAG
TACATGTTAAGTATCTTTTATTTGATATTTGGCTTGTCGCTGTTAGTGCCGATTATCGTGTGGCTGATACCTGCGTGGCG
CTCTATGTTAAGCCTGAATAAGGTCGCGATATTACTGGGGAAAGGCCATTTTGATACGCGTGCTAAATTTATTCATTTTA
GCCATTTAAATCACTTGTCCAACACCATTAATATTATGGCGGAGAAAATTCAGCGATTAATTTCCTCACATAAAAATCTT
GTAAATGCCGTCTCTCACGAATTACGCACACCTATCGCTCGGATAGAGTTTAATATTGAAATGCTGAGAAATAATACTCA
GGATAATAATCAATTGACGCAACTAGATCGTATAGAGTTTTCATTAAATGAGTTAAATTCATTAGTGTCGGAAATGCTTG
AACATGCGCGGTTTGATAGCGAAATACCGACACTGACATTTGAGTCAATTGAGCTCAACCACTGGCTAAGACAAGAGTTA
CTGCTGTGGCAAGAGGCTAACCCAACGATAGTCATCACTTTGCTTGGGCATGATGAATGCTTGGCAAGATTCGATAGGTT
TTACATGAGCCGCGCCATCAGTAATTTAGTCCGAAATGCCATTGCCTATGGGCAAAGCCAGATCCACGTCGGCTACCAAA
AAACAGCAAAAGGGTGGATGGTTTTCATTGAAGATGATGGTTATGGTGTGCCTTTGGCCTCCAGAGATAAAGTGTTTGAG
CCGTTTTATCGAGACGATGAAAGCCGCAATCTGCAAAAGAGTGGGACAGGATTAGGCCTAGCCATTGTCAAGCAAATCAT
AGATTGGCATAGCGGTTCGGCGAGCGTTAACGCGTCAGCTTTAGGAGGCGCTCGTTTTGTCCTCAGTTGGCCAGACAAGA
AGTTATGA

Upstream 100 bases:

>100_bases
GTGATAGATGAATAGCGACAACCCTTCCAATAGATTGTTTTCATTTTTGCATTGTTATTGAAATACCACTTTTCGAATAT
TTTTTCGTTATAGGCTACGT

Downstream 100 bases:

>100_bases
CGTATAGCAGGTTAAGGAGTAGGCGATAATGAAAACCAAAATTCTCATTGTAGAGGATGATTTGCCTTTAGCTGAGTCTA
TTCAGGAGTACCTTAACTAT

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKKSLLSLAIIVILSVVGIQLFIIPLFENKVRHPTEKWALELLTSGTANMLAVQLQRLPKQDRAAYLQNMQANFGYELAV
QDYQRDDFSIVQQQWIDQNNVVGEPLTDLVYRRIDADQLLVVIKPNTVPKYLVNDAQRWSMGVFYLITHALNKQPENNWH
SIIAVINEDFAYPVTLQSLGEIHENDEQMSDLYAGRIIALSTADSDAIGYPADRVMQRIGSSDKVIVLGPFSTELKPKVQ
YMLSIFYLIFGLSLLVPIIVWLIPAWRSMLSLNKVAILLGKGHFDTRAKFIHFSHLNHLSNTINIMAEKIQRLISSHKNL
VNAVSHELRTPIARIEFNIEMLRNNTQDNNQLTQLDRIEFSLNELNSLVSEMLEHARFDSEIPTLTFESIELNHWLRQEL
LLWQEANPTIVITLLGHDECLARFDRFYMSRAISNLVRNAIAYGQSQIHVGYQKTAKGWMVFIEDDGYGVPLASRDKVFE
PFYRDDESRNLQKSGTGLGLAIVKQIIDWHSGSASVNASALGGARFVLSWPDKKL

Sequences:

>Translated_535_residues
MKKSLLSLAIIVILSVVGIQLFIIPLFENKVRHPTEKWALELLTSGTANMLAVQLQRLPKQDRAAYLQNMQANFGYELAV
QDYQRDDFSIVQQQWIDQNNVVGEPLTDLVYRRIDADQLLVVIKPNTVPKYLVNDAQRWSMGVFYLITHALNKQPENNWH
SIIAVINEDFAYPVTLQSLGEIHENDEQMSDLYAGRIIALSTADSDAIGYPADRVMQRIGSSDKVIVLGPFSTELKPKVQ
YMLSIFYLIFGLSLLVPIIVWLIPAWRSMLSLNKVAILLGKGHFDTRAKFIHFSHLNHLSNTINIMAEKIQRLISSHKNL
VNAVSHELRTPIARIEFNIEMLRNNTQDNNQLTQLDRIEFSLNELNSLVSEMLEHARFDSEIPTLTFESIELNHWLRQEL
LLWQEANPTIVITLLGHDECLARFDRFYMSRAISNLVRNAIAYGQSQIHVGYQKTAKGWMVFIEDDGYGVPLASRDKVFE
PFYRDDESRNLQKSGTGLGLAIVKQIIDWHSGSASVNASALGGARFVLSWPDKKL
>Mature_535_residues
MKKSLLSLAIIVILSVVGIQLFIIPLFENKVRHPTEKWALELLTSGTANMLAVQLQRLPKQDRAAYLQNMQANFGYELAV
QDYQRDDFSIVQQQWIDQNNVVGEPLTDLVYRRIDADQLLVVIKPNTVPKYLVNDAQRWSMGVFYLITHALNKQPENNWH
SIIAVINEDFAYPVTLQSLGEIHENDEQMSDLYAGRIIALSTADSDAIGYPADRVMQRIGSSDKVIVLGPFSTELKPKVQ
YMLSIFYLIFGLSLLVPIIVWLIPAWRSMLSLNKVAILLGKGHFDTRAKFIHFSHLNHLSNTINIMAEKIQRLISSHKNL
VNAVSHELRTPIARIEFNIEMLRNNTQDNNQLTQLDRIEFSLNELNSLVSEMLEHARFDSEIPTLTFESIELNHWLRQEL
LLWQEANPTIVITLLGHDECLARFDRFYMSRAISNLVRNAIAYGQSQIHVGYQKTAKGWMVFIEDDGYGVPLASRDKVFE
PFYRDDESRNLQKSGTGLGLAIVKQIIDWHSGSASVNASALGGARFVLSWPDKKL

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=393, Percent_Identity=31.8066157760814, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1790346, Length=241, Percent_Identity=31.9502074688797, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=28.9655172413793, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786783, Length=243, Percent_Identity=28.8065843621399, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=256, Percent_Identity=27.34375, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.974025974026, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=27.3809523809524, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=26.0223048327138, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=29.2792792792793, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=24.031007751938, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082128, Length=247, Percent_Identity=23.8866396761134, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60957; Mature: 60957

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLSLAIIVILSVVGIQLFIIPLFENKVRHPTEKWALELLTSGTANMLAVQLQRLPK
CCHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCC
QDRAAYLQNMQANFGYELAVQDYQRDDFSIVQQQWIDQNNVVGEPLTDLVYRRIDADQLL
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
VVIKPNTVPKYLVNDAQRWSMGVFYLITHALNKQPENNWHSIIAVINEDFAYPVTLQSLG
EEECCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCEEHHHHH
EIHENDEQMSDLYAGRIIALSTADSDAIGYPADRVMQRIGSSDKVIVLGPFSTELKPKVQ
HHHCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHH
YMLSIFYLIFGLSLLVPIIVWLIPAWRSMLSLNKVAILLGKGHFDTRAKFIHFSHLNHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHEEEHHHHHHHH
NTINIMAEKIQRLISSHKNLVNAVSHELRTPIARIEFNIEMLRNNTQDNNQLTQLDRIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHCCCCCCCHHHHHHHHHH
SLNELNSLVSEMLEHARFDSEIPTLTFESIELNHWLRQELLLWQEANPTIVITLLGHDEC
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCHHH
LARFDRFYMSRAISNLVRNAIAYGQSQIHVGYQKTAKGWMVFIEDDGYGVPLASRDKVFE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEEECCCCCCCCCCCCCCCC
PFYRDDESRNLQKSGTGLGLAIVKQIIDWHSGSASVNASALGGARFVLSWPDKKL
CHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKSLLSLAIIVILSVVGIQLFIIPLFENKVRHPTEKWALELLTSGTANMLAVQLQRLPK
CCHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCC
QDRAAYLQNMQANFGYELAVQDYQRDDFSIVQQQWIDQNNVVGEPLTDLVYRRIDADQLL
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
VVIKPNTVPKYLVNDAQRWSMGVFYLITHALNKQPENNWHSIIAVINEDFAYPVTLQSLG
EEECCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCEEHHHHH
EIHENDEQMSDLYAGRIIALSTADSDAIGYPADRVMQRIGSSDKVIVLGPFSTELKPKVQ
HHHCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHH
YMLSIFYLIFGLSLLVPIIVWLIPAWRSMLSLNKVAILLGKGHFDTRAKFIHFSHLNHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHEEEHHHHHHHH
NTINIMAEKIQRLISSHKNLVNAVSHELRTPIARIEFNIEMLRNNTQDNNQLTQLDRIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHCCCCCCCHHHHHHHHHH
SLNELNSLVSEMLEHARFDSEIPTLTFESIELNHWLRQELLLWQEANPTIVITLLGHDEC
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCHHH
LARFDRFYMSRAISNLVRNAIAYGQSQIHVGYQKTAKGWMVFIEDDGYGVPLASRDKVFE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEEECCCCCCCCCCCCCCCC
PFYRDDESRNLQKSGTGLGLAIVKQIIDWHSGSASVNASALGGARFVLSWPDKKL
CHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]