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The map label for this gene is ycbU [H]

Identifier: 160873431

GI number: 160873431

Start: 345627

End: 346748

Strand: Direct

Name: ycbU [H]

Synonym: Sbal195_0305

Alternate gene names: 160873431

Gene position: 345627-346748 (Clockwise)

Preceding gene: 160873430

Following gene: 160873433

Centisome position: 6.46

GC content: 49.73

Gene sequence:

>1122_bases
ATGTTTGAAGACTTTAAAAAGGACTTTTACCTCGCGGGTCCAGGATATCTATTGAACCATTCAGTCGGTCGACCGTTAAA
ATCGACCGAACAGGTCTTCAAAGAAGCCTTTTTTGCGCCTTGGCAGGAATCGGGGCGTGAGCCTTGGGGCCAGTGGCTGA
GTGTGATTGATGATTTTACCTTTGCTTTATCCAAACTGTTTAACGGCCACCAAAAGGACTTTTGCCCTCAGGTAAATCTT
TCCAGTGCACTGACTAAAATCGTGATGTCACTCGACCGATTAAATCGCGAACACGCCGTTGTGTTGATGAGTGAGATTGA
TTTCCCCAGCATGGGTTTCGCCCTTAAAAAAGCGCTGCCAGCTAGCTGCGAAGTGCGTTTTATTCCTAAGGGACTCGATG
TTACTGACCCTAATGTGTGGGACGCACATATCAGTCGCGATGTGGATCTTGTGTTTGTCAGCCATGCTTACTCGAATACG
GGGCAGCAGGCGCCATTAGCCAATATTTTCACCATAGCCCGTGAGCGAGGCTGTTTGAGCTTAGTGGATGTAGCGCAATC
GGCGGGGATTATTCCGCTAGATCTCGCGCGCCTACAACCTGATTTTTTAATCGGGTCGAGTGTGAAATGGCTCTGCGGCG
GCCCTGGTGCCGCCTATCTTTGGGTTAATCCCGCCATATTGGATAGTTGTCAGCCAAAGGATGTGGGGTGGTTTAGCCAC
GAGAATCCGTTCGAGTTCGATATCCATGATTTTCGTTACCAGCATTCCGCAATGCGCTTCTGGGGTGGAACGCCTTCAAT
CGCGCCCTATGCCATTGCCGCCCACAGCATTCATTACCTTGCGGGGTTAGGTGTTCCTTTGCTGCGTAAATGCAATCAGC
AGTTAATTGATGCCGTGGCGCAAGAGCTCGAACATGAGTTTGTGTCGCCACGGGATGTTGAAAAGCGCAGCGGTACGCTG
ATTTTGCAGTTTGGTGAGCAACAACATCAAATCATGACGGCATTCGCGAACGCGAACATCAGCGTCGATGCTCGCAGCAT
GGGGATCCGTATTTCGCCGCATATCTATAACAATATGGCCGATATCAAGCTGTTACTGGACGTGATAAAAGCCAATCGGT
AG

Upstream 100 bases:

>100_bases
TTGGAGCGACTCAGAGATAGGCGCGCCGCCGCTCAGCGCAGCGATATTCGGACGCGTTATTATGAGATTAAAAAATTAAA
AGAGAGTTTGAGATAACACT

Downstream 100 bases:

>100_bases
CCAATTGATAAGAGCTCTGTGGATAAATAGCTATGCCCAAACCTGAGTGTTTGGGCATTTTTTTAGAGTCGTTTACGATT
TAGCCGCTCGATGATCTGGC

Product: class V aminotransferase

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MFEDFKKDFYLAGPGYLLNHSVGRPLKSTEQVFKEAFFAPWQESGREPWGQWLSVIDDFTFALSKLFNGHQKDFCPQVNL
SSALTKIVMSLDRLNREHAVVLMSEIDFPSMGFALKKALPASCEVRFIPKGLDVTDPNVWDAHISRDVDLVFVSHAYSNT
GQQAPLANIFTIARERGCLSLVDVAQSAGIIPLDLARLQPDFLIGSSVKWLCGGPGAAYLWVNPAILDSCQPKDVGWFSH
ENPFEFDIHDFRYQHSAMRFWGGTPSIAPYAIAAHSIHYLAGLGVPLLRKCNQQLIDAVAQELEHEFVSPRDVEKRSGTL
ILQFGEQQHQIMTAFANANISVDARSMGIRISPHIYNNMADIKLLLDVIKANR

Sequences:

>Translated_373_residues
MFEDFKKDFYLAGPGYLLNHSVGRPLKSTEQVFKEAFFAPWQESGREPWGQWLSVIDDFTFALSKLFNGHQKDFCPQVNL
SSALTKIVMSLDRLNREHAVVLMSEIDFPSMGFALKKALPASCEVRFIPKGLDVTDPNVWDAHISRDVDLVFVSHAYSNT
GQQAPLANIFTIARERGCLSLVDVAQSAGIIPLDLARLQPDFLIGSSVKWLCGGPGAAYLWVNPAILDSCQPKDVGWFSH
ENPFEFDIHDFRYQHSAMRFWGGTPSIAPYAIAAHSIHYLAGLGVPLLRKCNQQLIDAVAQELEHEFVSPRDVEKRSGTL
ILQFGEQQHQIMTAFANANISVDARSMGIRISPHIYNNMADIKLLLDVIKANR
>Mature_373_residues
MFEDFKKDFYLAGPGYLLNHSVGRPLKSTEQVFKEAFFAPWQESGREPWGQWLSVIDDFTFALSKLFNGHQKDFCPQVNL
SSALTKIVMSLDRLNREHAVVLMSEIDFPSMGFALKKALPASCEVRFIPKGLDVTDPNVWDAHISRDVDLVFVSHAYSNT
GQQAPLANIFTIARERGCLSLVDVAQSAGIIPLDLARLQPDFLIGSSVKWLCGGPGAAYLWVNPAILDSCQPKDVGWFSH
ENPFEFDIHDFRYQHSAMRFWGGTPSIAPYAIAAHSIHYLAGLGVPLLRKCNQQLIDAVAQELEHEFVSPRDVEKRSGTL
ILQFGEQQHQIMTAFANANISVDARSMGIRISPHIYNNMADIKLLLDVIKANR

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 41747; Mature: 41747

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEDFKKDFYLAGPGYLLNHSVGRPLKSTEQVFKEAFFAPWQESGREPWGQWLSVIDDFT
CCCCCCCCEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHH
FALSKLFNGHQKDFCPQVNLSSALTKIVMSLDRLNREHAVVLMSEIDFPSMGFALKKALP
HHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCC
ASCEVRFIPKGLDVTDPNVWDAHISRDVDLVFVSHAYSNTGQQAPLANIFTIARERGCLS
CCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEEEEHHCCCCCCCCHHHHHHHHHHCCCHH
LVDVAQSAGIIPLDLARLQPDFLIGSSVKWLCGGPGAAYLWVNPAILDSCQPKDVGWFSH
HHHHHHHCCCCEEHHHHCCCCEEECCCCCEEECCCCEEEEEECHHHHCCCCCCCCCCCCC
ENPFEFDIHDFRYQHSAMRFWGGTPSIAPYAIAAHSIHYLAGLGVPLLRKCNQQLIDAVA
CCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QELEHEFVSPRDVEKRSGTLILQFGEQQHQIMTAFANANISVDARSMGIRISPHIYNNMA
HHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEHHHCCEEECCHHHCCHH
DIKLLLDVIKANR
HHHHHHHHHHCCC
>Mature Secondary Structure
MFEDFKKDFYLAGPGYLLNHSVGRPLKSTEQVFKEAFFAPWQESGREPWGQWLSVIDDFT
CCCCCCCCEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHH
FALSKLFNGHQKDFCPQVNLSSALTKIVMSLDRLNREHAVVLMSEIDFPSMGFALKKALP
HHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCC
ASCEVRFIPKGLDVTDPNVWDAHISRDVDLVFVSHAYSNTGQQAPLANIFTIARERGCLS
CCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEEEEHHCCCCCCCCHHHHHHHHHHCCCHH
LVDVAQSAGIIPLDLARLQPDFLIGSSVKWLCGGPGAAYLWVNPAILDSCQPKDVGWFSH
HHHHHHHCCCCEEHHHHCCCCEEECCCCCEEECCCCEEEEEECHHHHCCCCCCCCCCCCC
ENPFEFDIHDFRYQHSAMRFWGGTPSIAPYAIAAHSIHYLAGLGVPLLRKCNQQLIDAVA
CCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QELEHEFVSPRDVEKRSGTLILQFGEQQHQIMTAFANANISVDARSMGIRISPHIYNNMA
HHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEHHHCCEEECCHHHCCHH
DIKLLLDVIKANR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]