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The map label for this gene is dcuB [H]

Identifier: 160873429

GI number: 160873429

Start: 342835

End: 344178

Strand: Reverse

Name: dcuB [H]

Synonym: Sbal195_0303

Alternate gene names: 160873429

Gene position: 344178-342835 (Counterclockwise)

Preceding gene: 160873432

Following gene: 160873428

Centisome position: 6.44

GC content: 49.03

Gene sequence:

>1344_bases
ATGATTTTAATGGAATTTATAGTAGTGCTTAGTTGCCTACTACTCGGTACCCGCTACGGCGGTATGGGACTCGGCCTCAT
CAGCGGCATAGGTTTGTTTTTACTGATATTTGTCTTCGGTTTAGCGCCGGGACAACCGCCAGTACAAGTTATGCTAACGA
TTCTTGCGGTGATCGGCTGTGCGGCCGTATTACAAACCGCAGGCGGTTTGGATGTGATGATGCAGTTTGCCGAGCGCCTA
CTGCGCCGCCATCCACAATACATCACCATTCTGGCGCCGCTGACCACTTGGACGCTGACCTTTCTTTGCGGTACAGGCCA
TGTGGTTTACACCATGTTTCCGATTATTTCCGATATCGCGCTGAAGAAAAATATTCGCCCAGAACGCCCAATGGCTGTGG
CATCCGTCGCCTCGCAAATGGCAATTTGTGCTTCGCCCGTGTCGGTAGCGGTTGTGTCTATGGTGTCGATTCTCGCATCG
CAGCAAGGTGTAGGCCGCGCCTACAATCTGCTCGAAATTTTAATGGTATCCGTACCGGCATCGCTTTGCGGTGTGATGGC
CGCCGCACTTTGGAGCCTACGTCGCGGTAAAGATTTAGATAAAGACGAAGAGTTCCAAGAACGGATTCAAGATCCTGTCC
AAAGAGCTTTTATCTATGACAAGAGCGAAACCCTGCTAAATCAACGTTTTCCTAAGGAAGCTTACTGGGCAACGGGAATT
TTCTTTGCGGCTATTGCCGCTGTGGTGCTGTTAGGTTCCTTTAGTGAACTGCGCCCAGCCTTTGAAGTTAAAGGAAAAAC
GACCTTATTATCGATGAACTTAGTCATCCAGATGATGATGTTAATTGCGGGGGCATTTATCCTGATGACCTGCAAAGTCA
AACCTACAAACATCTCTAATGGCCCCGTCTTTAAAGCGGGCATGGTGGCGATTTTCTCAGTATTTGGGGTGGCGTGGATG
AGTGATACTTTCTTCATCAGCCATATGGATGTGCTTAAAGAAAACCTGTCCCACGTGGTGCAAAGCCAGCCTTGGACCTA
TGCCTTAGTGCTGTTCCTTATCTCTAAGCTTGTTAATAGCCAAGCGGCGGCGCTGACTGCCATTGCGCCTATGGGTCTCG
CCTTAGGCGTCGATCCAAAACTCTTGATCGCTTTCCTTCCTGCAAGTTACGGTTATTTTGTGCTCCCTACTTATCCAAGC
GATCTGGCCTGTATCGGCTTTGACCGTTCAGGCACAACTAAAATAGGTAAGTTCATTATTAACCACAGCTTTATCATTCC
AGGTTTTATCGGCGTGGGTACCGCATCGACCGTCGGTTATCTACTGGCAACAGCCTTGCTCTAG

Upstream 100 bases:

>100_bases
CCAGCGCAATTTTTCAAACGCTTTTCCTCCGCAGAATGTCCCAGAAAACACTCATCACTCAACAAATCGCTAAGCGGTAG
ATTCCGCAGAGGTAAATCGG

Downstream 100 bases:

>100_bases
TCTGTATTACATAAATAATAAAGGCCGCAGATGCGGCCTTTATGTTGAATAGCATGGAACGCTAAGTTTAAGCGCGATAC
ATGGCCAAAAACATCTTGAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MILMEFIVVLSCLLLGTRYGGMGLGLISGIGLFLLIFVFGLAPGQPPVQVMLTILAVIGCAAVLQTAGGLDVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAS
QQGVGRAYNLLEILMVSVPASLCGVMAAALWSLRRGKDLDKDEEFQERIQDPVQRAFIYDKSETLLNQRFPKEAYWATGI
FFAAIAAVVLLGSFSELRPAFEVKGKTTLLSMNLVIQMMMLIAGAFILMTCKVKPTNISNGPVFKAGMVAIFSVFGVAWM
SDTFFISHMDVLKENLSHVVQSQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTKIGKFIINHSFIIPGFIGVGTASTVGYLLATALL

Sequences:

>Translated_447_residues
MILMEFIVVLSCLLLGTRYGGMGLGLISGIGLFLLIFVFGLAPGQPPVQVMLTILAVIGCAAVLQTAGGLDVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAS
QQGVGRAYNLLEILMVSVPASLCGVMAAALWSLRRGKDLDKDEEFQERIQDPVQRAFIYDKSETLLNQRFPKEAYWATGI
FFAAIAAVVLLGSFSELRPAFEVKGKTTLLSMNLVIQMMMLIAGAFILMTCKVKPTNISNGPVFKAGMVAIFSVFGVAWM
SDTFFISHMDVLKENLSHVVQSQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTKIGKFIINHSFIIPGFIGVGTASTVGYLLATALL
>Mature_447_residues
MILMEFIVVLSCLLLGTRYGGMGLGLISGIGLFLLIFVFGLAPGQPPVQVMLTILAVIGCAAVLQTAGGLDVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAS
QQGVGRAYNLLEILMVSVPASLCGVMAAALWSLRRGKDLDKDEEFQERIQDPVQRAFIYDKSETLLNQRFPKEAYWATGI
FFAAIAAVVLLGSFSELRPAFEVKGKTTLLSMNLVIQMMMLIAGAFILMTCKVKPTNISNGPVFKAGMVAIFSVFGVAWM
SDTFFISHMDVLKENLSHVVQSQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTKIGKFIINHSFIIPGFIGVGTASTVGYLLATALL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=407, Percent_Identity=55.7739557739558, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1790580, Length=449, Percent_Identity=36.5256124721604, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 48271; Mature: 48271

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMEFIVVLSCLLLGTRYGGMGLGLISGIGLFLLIFVFGLAPGQPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AAVLQTAGGLDVMMQFAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILASQQGVGRAYNLLEILMVSVPA
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
SLCGVMAAALWSLRRGKDLDKDEEFQERIQDPVQRAFIYDKSETLLNQRFPKEAYWATGI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
FFAAIAAVVLLGSFSELRPAFEVKGKTTLLSMNLVIQMMMLIAGAFILMTCKVKPTNISN
HHHHHHHHHHHCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
GPVFKAGMVAIFSVFGVAWMSDTFFISHMDVLKENLSHVVQSQPWTYALVLFLISKLVNS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPSDLACIGFDRSGTTKIGKFII
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGFIGVGTASTVGYLLATALL
CCCEECCCHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MILMEFIVVLSCLLLGTRYGGMGLGLISGIGLFLLIFVFGLAPGQPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AAVLQTAGGLDVMMQFAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILASQQGVGRAYNLLEILMVSVPA
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
SLCGVMAAALWSLRRGKDLDKDEEFQERIQDPVQRAFIYDKSETLLNQRFPKEAYWATGI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
FFAAIAAVVLLGSFSELRPAFEVKGKTTLLSMNLVIQMMMLIAGAFILMTCKVKPTNISN
HHHHHHHHHHHCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
GPVFKAGMVAIFSVFGVAWMSDTFFISHMDVLKENLSHVVQSQPWTYALVLFLISKLVNS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPSDLACIGFDRSGTTKIGKFII
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGFIGVGTASTVGYLLATALL
CCCEECCCHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]