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The map label for this gene is ycdT [H]

Identifier: 160873406

GI number: 160873406

Start: 320428

End: 321888

Strand: Direct

Name: ycdT [H]

Synonym: Sbal195_0280

Alternate gene names: 160873406

Gene position: 320428-321888 (Clockwise)

Preceding gene: 160873405

Following gene: 160873407

Centisome position: 5.99

GC content: 44.35

Gene sequence:

>1461_bases
ATGGGCAATAACGCTTGGAATGATAAGAATAAATTTATTTGGCTGCTCTCTGTTTTGCTTTTAGTCGCCTTCTTAGTGAC
CAGTGGTATCAGTTATAAGGTGGCCCATGACTCACTGAGTCAACAAATCGAGAGTAATACCCTCCCGCTCACAAGTGACA
ATATTTACTCAGAAATTCAACAGGACCTGCTGCGACCTATCTTTATTTCATCCTTGATGGCGCAGGATACTTTTGTTCGC
GATTGGACATTAAGCCACGAGCAAGAGCCAGAAAAACTGATCCGTTACCTTAAGGAAATCCAAGAGAAATACGATACAGT
TACCAGTTTTTTTGTGTCAGAGAAAAGTCGGCATTATTACCACTCCACGGGCATTTTAAAACAAATCCAAGATGTTGAGC
CCGATGATGCTTGGTACTTTCGAGTGCGCTCTTTACCCGAATCTGAGCATTATGAGGTCAATATTGATGCTGATACGGCG
GACCGCAGTAAAACAACCGTATTCGTAAACTATAAAGTTTTTGATTTTGAAGGCAAATTTATAGGCATTACCGGTGTGGG
TTTGGCGGTTGAAAAGGTTAAGTCCCTCATCGAGCTTTATCAAAAACGTTATAACCGTCGAGTGTTCTTTACCGATCGGC
AGGGCAATGTCACTTTGCATGGCGATGAATATGATGGTGCGGATTCGCTGCAAACCAGTCCAGGGCTTGAAAATTTAGCG
ACGCGTATTTTAACCAGTCCAAGTGCGGCTTTTAGCTACCAGAGAAATGGTAAAACCGTTTATCTCAACAGCCGACTAGT
CCCTGAGTTTAAGTGGTACTTGATTGTTGAGCAGGAAGATGCGCCTCAGGAGCGGGAGTTACTGAATACTTTTTGGGGCA
ACTTGGGCTTAAGCCTAATGGTGACCCTAGGGATTTTATTTATCTCCAACATGACCTTAGGTCGTTATCAGCGCAAATTA
GAAGTAATGGCGTCTACCGATAAGCTTACTGGCGCCGCTAACCGTCAGGTGTTTGATGAGTACTTTAGACAGGCGTTAGA
TAAGGCTAAGTTGGCACAATCGCCGATTTCAATACTCTTGCTGGATATTGACCACTTCAAAAAAGTGAACGATAGCTATG
GGCATGGGATTGGTGACTTAGTATTAAAAACCATGACGAATATGTTGCGTGGCGCGCTGCAGCAAGAGGATATCTTATGT
CGTTGGGGCGGCGAGGAGTTCTTGATTTTACTTCCGGGGATGGATTTAACCCGAGCGGCAGAACTGGCTGAACAGATCCG
TGAGCTTATTTTTCAGCGGGAAATTAAAGTGAATGGATTACATATTTCTATTACTGCCAGTATCGGCGTCGCCGAGCATC
AGGCGCAAGAGCCTGCAGAAGATCTGATCAAACGTGCCGATCTGGCCTTGTATCAGGCTAAGGAAGCCGGTCGAAATCAA
GTGGTGTTAAATCATTCATAG

Upstream 100 bases:

>100_bases
GCCATGGCGCAATTGCGTTAACATTTATTCAACTAAAATAGTTATCGTGACATTAAAATAATAAGCCACTCAAGGTGTTC
TGTAGTCTTTGGAGTTGAGA

Downstream 100 bases:

>100_bases
ATTAAGCCCCGTGCTTATTGGCGGCAAGATTGCGCCTACTTTTATGAGTTATTGAGCGAGTTGATTGTGTTACTGGCGTA
TCGCGGTTTACACTCTGGCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNNAWNDKNKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTLSHEQEPEKLIRYLKEIQEKYDTVTSFFVSEKSRHYYHSTGILKQIQDVEPDDAWYFRVRSLPESEHYEVNIDADTA
DRSKTTVFVNYKVFDFEGKFIGITGVGLAVEKVKSLIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSPGLENLA
TRILTSPSAAFSYQRNGKTVYLNSRLVPEFKWYLIVEQEDAPQERELLNTFWGNLGLSLMVTLGILFISNMTLGRYQRKL
EVMASTDKLTGAANRQVFDEYFRQALDKAKLAQSPISILLLDIDHFKKVNDSYGHGIGDLVLKTMTNMLRGALQQEDILC
RWGGEEFLILLPGMDLTRAAELAEQIRELIFQREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKEAGRNQ
VVLNHS

Sequences:

>Translated_486_residues
MGNNAWNDKNKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTLSHEQEPEKLIRYLKEIQEKYDTVTSFFVSEKSRHYYHSTGILKQIQDVEPDDAWYFRVRSLPESEHYEVNIDADTA
DRSKTTVFVNYKVFDFEGKFIGITGVGLAVEKVKSLIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSPGLENLA
TRILTSPSAAFSYQRNGKTVYLNSRLVPEFKWYLIVEQEDAPQERELLNTFWGNLGLSLMVTLGILFISNMTLGRYQRKL
EVMASTDKLTGAANRQVFDEYFRQALDKAKLAQSPISILLLDIDHFKKVNDSYGHGIGDLVLKTMTNMLRGALQQEDILC
RWGGEEFLILLPGMDLTRAAELAEQIRELIFQREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKEAGRNQ
VVLNHS
>Mature_485_residues
GNNAWNDKNKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVRD
WTLSHEQEPEKLIRYLKEIQEKYDTVTSFFVSEKSRHYYHSTGILKQIQDVEPDDAWYFRVRSLPESEHYEVNIDADTAD
RSKTTVFVNYKVFDFEGKFIGITGVGLAVEKVKSLIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSPGLENLAT
RILTSPSAAFSYQRNGKTVYLNSRLVPEFKWYLIVEQEDAPQERELLNTFWGNLGLSLMVTLGILFISNMTLGRYQRKLE
VMASTDKLTGAANRQVFDEYFRQALDKAKLAQSPISILLLDIDHFKKVNDSYGHGIGDLVLKTMTNMLRGALQQEDILCR
WGGEEFLILLPGMDLTRAAELAEQIRELIFQREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKEAGRNQV
VLNHS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=41.566265060241, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=41.4201183431953, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=36.6834170854271, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=37.3493975903614, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=32.8125, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=35.4430379746835, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=31.9767441860465, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=32.4324324324324, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=197, Percent_Identity=29.4416243654822, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=33.125, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=30.1775147928994, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=189, Percent_Identity=24.8677248677249, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=33.5403726708075, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081921, Length=137, Percent_Identity=27.007299270073, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55528; Mature: 55397

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNAWNDKNKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTLSHEQEPEKLIRYLKEIQEKYDTVTSFFVSEKSRHYY
HHHHHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HSTGILKQIQDVEPDDAWYFRVRSLPESEHYEVNIDADTADRSKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGITGVGLAVEKVKSLIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSPGLENLA
EEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHH
TRILTSPSAAFSYQRNGKTVYLNSRLVPEFKWYLIVEQEDAPQERELLNTFWGNLGLSLM
HHHHCCCCHHEEEECCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VTLGILFISNMTLGRYQRKLEVMASTDKLTGAANRQVFDEYFRQALDKAKLAQSPISILL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
LDIDHFKKVNDSYGHGIGDLVLKTMTNMLRGALQQEDILCRWGGEEFLILLPGMDLTRAA
EEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHH
ELAEQIRELIFQREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKEAGRNQ
HHHHHHHHHHHHHCEEECCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNHS
EEEECC
>Mature Secondary Structure 
GNNAWNDKNKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTLSHEQEPEKLIRYLKEIQEKYDTVTSFFVSEKSRHYY
HHHHHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HSTGILKQIQDVEPDDAWYFRVRSLPESEHYEVNIDADTADRSKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGITGVGLAVEKVKSLIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSPGLENLA
EEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHH
TRILTSPSAAFSYQRNGKTVYLNSRLVPEFKWYLIVEQEDAPQERELLNTFWGNLGLSLM
HHHHCCCCHHEEEECCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VTLGILFISNMTLGRYQRKLEVMASTDKLTGAANRQVFDEYFRQALDKAKLAQSPISILL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
LDIDHFKKVNDSYGHGIGDLVLKTMTNMLRGALQQEDILCRWGGEEFLILLPGMDLTRAA
EEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHH
ELAEQIRELIFQREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKEAGRNQ
HHHHHHHHHHHHHCEEECCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNHS
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]