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The map label for this gene is def [H]

Identifier: 160873157

GI number: 160873157

Start: 36203

End: 36715

Strand: Reverse

Name: def [H]

Synonym: Sbal195_0031

Alternate gene names: 160873157

Gene position: 36715-36203 (Counterclockwise)

Preceding gene: 160873164

Following gene: 160873156

Centisome position: 0.69

GC content: 43.27

Gene sequence:

>513_bases
ATGGCACAATTAAAAGTATTACAATTTCCTGACGAAAGATTGCGGACACAAGCCACACCCATCACAGAGTTTAATGCCGA
GCTGCAAACTCAAATTGATGATATGTTCGAAACCATGTACCAAGAAAAAGGTATCGGTCTGGCCGCGACCCAAGTGGATT
ACCATAAACAACTTATTGTCATGGACTTACAAGATGAAGTCGAACGACCTAAAGTGTTTATTAATCCCGAAATTATCGCC
AGCAGCGGTGACTTCTGTAATGAAGAAGGCTGCCTGTCGGTTCCGGGCATTTATGCCAAAGTCGATCGCGCCGAATTTGT
TACGGTAAAAGCCTTAGACAGGCACGGCAATGAATTTATTGTTGAGGCAGATGAGCTTTTTGCTATCTGTATTCAACACG
AAATGGATCACCTCAAAGGCAAATTGTTTGTCGATTATCTGTCGCCGCTAAAACGTCAGCGGATCAAACAAAAACTTGAA
AAAGCGGCCCGATTGGACGCTAAAGAAGCATAG

Upstream 100 bases:

>100_bases
TTACTGTTATTGATAGGCACTAAAGATTAAAATTAACCCATTAGCATCAGTCAGTATAACTAACTGGCAGAGATTTGACA
GACTTGTTAAGAGTTTATGT

Downstream 100 bases:

>100_bases
GATCAGATTTGAAACCACTCAACATCATCTTTGCCGGAACACCGGATTTTGCCGCTCGCCACTTACAAGCACTGTTGAAC
TCACACCACAATGTGATTGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MAQLKVLQFPDERLRTQATPITEFNAELQTQIDDMFETMYQEKGIGLAATQVDYHKQLIVMDLQDEVERPKVFINPEIIA
SSGDFCNEEGCLSVPGIYAKVDRAEFVTVKALDRHGNEFIVEADELFAICIQHEMDHLKGKLFVDYLSPLKRQRIKQKLE
KAARLDAKEA

Sequences:

>Translated_170_residues
MAQLKVLQFPDERLRTQATPITEFNAELQTQIDDMFETMYQEKGIGLAATQVDYHKQLIVMDLQDEVERPKVFINPEIIA
SSGDFCNEEGCLSVPGIYAKVDRAEFVTVKALDRHGNEFIVEADELFAICIQHEMDHLKGKLFVDYLSPLKRQRIKQKLE
KAARLDAKEA
>Mature_169_residues
AQLKVLQFPDERLRTQATPITEFNAELQTQIDDMFETMYQEKGIGLAATQVDYHKQLIVMDLQDEVERPKVFINPEIIAS
SGDFCNEEGCLSVPGIYAKVDRAEFVTVKALDRHGNEFIVEADELFAICIQHEMDHLKGKLFVDYLSPLKRQRIKQKLEK
AARLDAKEA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=31.875, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=60.7142857142857, Blast_Score=206, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=29.375, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19474; Mature: 19342

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLKVLQFPDERLRTQATPITEFNAELQTQIDDMFETMYQEKGIGLAATQVDYHKQLIV
CCCCEEEECCHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCEEEE
MDLQDEVERPKVFINPEIIASSGDFCNEEGCLSVPGIYAKVDRAEFVTVKALDRHGNEFI
EECHHHHCCCEEEECCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEE
VEADELFAICIQHEMDHLKGKLFVDYLSPLKRQRIKQKLEKAARLDAKEA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AQLKVLQFPDERLRTQATPITEFNAELQTQIDDMFETMYQEKGIGLAATQVDYHKQLIV
CCCEEEECCHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCEEEE
MDLQDEVERPKVFINPEIIASSGDFCNEEGCLSVPGIYAKVDRAEFVTVKALDRHGNEFI
EECHHHHCCCEEEECCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEE
VEADELFAICIQHEMDHLKGKLFVDYLSPLKRQRIKQKLEKAARLDAKEA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]