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The map label for this gene is thiF [H]

Identifier: 159901388

GI number: 159901388

Start: 6212214

End: 6213245

Strand: Direct

Name: thiF [H]

Synonym: Haur_4877

Alternate gene names: 159901388

Gene position: 6212214-6213245 (Clockwise)

Preceding gene: 159901386

Following gene: 159901389

Centisome position: 97.88

GC content: 54.94

Gene sequence:

>1032_bases
ATGCCCAGTAGTGGGATACCATCAGCACGCTATAGTCGTCAAACGCGGTTTGCTGGGCTAGGCCAAGCCGGGCAGCAGCG
CTTAGCCCAAGCACGAGTGGCGATTGTTGGCTTGGGTGCAACCGGTAGCACCATCGCCCATGCCTTGCTACGAGCGGGCG
TAGGCTATTTGCGGCTAATCGACCGCGATTGGGTTGAGGAGCATAATTTGCCGCGCCAAAGTTTGTATACCGAGGCTGAT
GCTGCTCAGTTAGTGCCCAAAGTTGTGGCTGCCAAAGCCCATGCCCAGCGCATCAACAGTGCTTGCGACATTGAGGCGTT
GGTGCTCGATTTACATGCTGGCACGATTGATCAAGCACTAGCTGGGGTCGATTTAATTATGGATGGCAGCGATAGCCTCG
AAACCCGCTTGCTGATCAATCAATGGTGTGTGCGTGAAGGCAAGCCATGGATTTATAGTGGCGTGTTGGGTGGCCATGGC
ATGACCGCAAATTTTCGGCCCAAGCAAGCCTGTTGGCGCTGTGTTTTTACGACTTCGCCGGAGCCAGGCAGCATGCCAAC
GTGTGAAACCGCTGGCGTGATTGGGCCAGTCGTGGGTGTTATTGGCAATTTGGCGGCAACTGAAGCACTTAAATTGCTCA
GTGGGCAAGGCCAAGCCAACCCCGATTTATATATGCTCGATCTGTGGGCTTGGCAATTTGAGCAGCTGCCGCTGCCAACG
CCGCGCCCCGATTGCCCAGTTTGTGGCTTGCGCCAATTCGATTTGCTGGAGCAAGATAGTGCGCCAACCCTGAGTTTATG
TGGCCGCAACGCCATCCAAATTCGGCCACAACAGCCAATCACCATGGCCTTAGCCCAATTGGCAGCCCATTTGCAGCAAG
CCGATCTGCGAGTGATTCAAACCGACTATCTGCTACGCTTTGCGGCTGAAACCTTGCAGGCCACCGTGTTTCCTGATGGC
CGCGTGATTATCAGCGGCACCGATGATCCAGCGCTGGCACGCGGATTTTACAATCGCTGGATTAACCATTAA

Upstream 100 bases:

>100_bases
AACAATTAAATACGATTTTACGAGGCGGTATCAATCTCTGTCAAGTTGATACTGTCTCACCTGCGCGGTACAATGAGCCA
CAATGCAAATAGGGGGTTTT

Downstream 100 bases:

>100_bases
TCGATAATTTCATGTTCAAGGTTGAACGTCTATGCTGGAGCAGTTATGATTCTGCGATTTGCGCTGGTTTTAGCCATTGG
CACCAGCATCACTTTTATTT

Product: UBA/THIF-type NAD/FAD binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MPSSGIPSARYSRQTRFAGLGQAGQQRLAQARVAIVGLGATGSTIAHALLRAGVGYLRLIDRDWVEEHNLPRQSLYTEAD
AAQLVPKVVAAKAHAQRINSACDIEALVLDLHAGTIDQALAGVDLIMDGSDSLETRLLINQWCVREGKPWIYSGVLGGHG
MTANFRPKQACWRCVFTTSPEPGSMPTCETAGVIGPVVGVIGNLAATEALKLLSGQGQANPDLYMLDLWAWQFEQLPLPT
PRPDCPVCGLRQFDLLEQDSAPTLSLCGRNAIQIRPQQPITMALAQLAAHLQQADLRVIQTDYLLRFAAETLQATVFPDG
RVIISGTDDPALARGFYNRWINH

Sequences:

>Translated_343_residues
MPSSGIPSARYSRQTRFAGLGQAGQQRLAQARVAIVGLGATGSTIAHALLRAGVGYLRLIDRDWVEEHNLPRQSLYTEAD
AAQLVPKVVAAKAHAQRINSACDIEALVLDLHAGTIDQALAGVDLIMDGSDSLETRLLINQWCVREGKPWIYSGVLGGHG
MTANFRPKQACWRCVFTTSPEPGSMPTCETAGVIGPVVGVIGNLAATEALKLLSGQGQANPDLYMLDLWAWQFEQLPLPT
PRPDCPVCGLRQFDLLEQDSAPTLSLCGRNAIQIRPQQPITMALAQLAAHLQQADLRVIQTDYLLRFAAETLQATVFPDG
RVIISGTDDPALARGFYNRWINH
>Mature_342_residues
PSSGIPSARYSRQTRFAGLGQAGQQRLAQARVAIVGLGATGSTIAHALLRAGVGYLRLIDRDWVEEHNLPRQSLYTEADA
AQLVPKVVAAKAHAQRINSACDIEALVLDLHAGTIDQALAGVDLIMDGSDSLETRLLINQWCVREGKPWIYSGVLGGHGM
TANFRPKQACWRCVFTTSPEPGSMPTCETAGVIGPVVGVIGNLAATEALKLLSGQGQANPDLYMLDLWAWQFEQLPLPTP
RPDCPVCGLRQFDLLEQDSAPTLSLCGRNAIQIRPQQPITMALAQLAAHLQQADLRVIQTDYLLRFAAETLQATVFPDGR
VIISGTDDPALARGFYNRWINH

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=266, Percent_Identity=37.218045112782, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=35.3909465020576, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=38.0165289256198, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540406, Length=251, Percent_Identity=30.6772908366534, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321903, Length=209, Percent_Identity=32.5358851674641, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24582879, Length=242, Percent_Identity=33.0578512396694, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 37179; Mature: 37048

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSGIPSARYSRQTRFAGLGQAGQQRLAQARVAIVGLGATGSTIAHALLRAGVGYLRLI
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCHHEEHH
DRDWVEEHNLPRQSLYTEADAAQLVPKVVAAKAHAQRINSACDIEALVLDLHAGTIDQAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
AGVDLIMDGSDSLETRLLINQWCVREGKPWIYSGVLGGHGMTANFRPKQACWRCVFTTSP
HCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHEEEEEECCC
EPGSMPTCETAGVIGPVVGVIGNLAATEALKLLSGQGQANPDLYMLDLWAWQFEQLPLPT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCCCC
PRPDCPVCGLRQFDLLEQDSAPTLSLCGRNAIQIRPQQPITMALAQLAAHLQQADLRVIQ
CCCCCCCCCCHHHHHHCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
TDYLLRFAAETLQATVFPDGRVIISGTDDPALARGFYNRWINH
HHHHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSSGIPSARYSRQTRFAGLGQAGQQRLAQARVAIVGLGATGSTIAHALLRAGVGYLRLI
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCHHEEHH
DRDWVEEHNLPRQSLYTEADAAQLVPKVVAAKAHAQRINSACDIEALVLDLHAGTIDQAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
AGVDLIMDGSDSLETRLLINQWCVREGKPWIYSGVLGGHGMTANFRPKQACWRCVFTTSP
HCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHEEEEEECCC
EPGSMPTCETAGVIGPVVGVIGNLAATEALKLLSGQGQANPDLYMLDLWAWQFEQLPLPT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCCCC
PRPDCPVCGLRQFDLLEQDSAPTLSLCGRNAIQIRPQQPITMALAQLAAHLQQADLRVIQ
CCCCCCCCCCHHHHHHCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
TDYLLRFAAETLQATVFPDGRVIISGTDDPALARGFYNRWINH
HHHHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]