Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 159901112

GI number: 159901112

Start: 5881776

End: 5884052

Strand: Reverse

Name: pcrA [H]

Synonym: Haur_4600

Alternate gene names: 159901112

Gene position: 5884052-5881776 (Counterclockwise)

Preceding gene: 159901113

Following gene: 159901111

Centisome position: 92.71

GC content: 51.6

Gene sequence:

>2277_bases
ATGACTCACCCGCTGCTGATTGGGTTGAATGCCCAACAACAACGCGCAGTCCAAGCGATTCATGGGCCAGTGTTGGTGCT
GGCTGGCCCAGGCTCGGGCAAGACCCGCGTGCTGACTCATCGGATTGCCTATTTAATTAATGAAGTTGGGGTTCGCCCCT
ATAACATTTTAGCCGTCACCTTTACCAACAAGGCTGCCCGCGAAATGCGCGAACGGATTGGAAACTTGATTGGCGAAAGT
CGTGCTCACGATGTAATGATGGGTACATTCCACTCAATTTGCGCCCGTTGGTTGCGCCGCGATATTCAGCATCTGCAACG
CGCCAACGATTTTGTGGTTTACGATGCCGATGATCAGCAACGGGTAATGAAGCAGATTTTGCGCGAATTGGATTTAAGCG
AGAAGCAATATAATCCGCGTTCAATTCATGCCCGCATCTCCGCTGCTAAAAACGAAATGATTGGGGTTGCTGAGTTTGCC
CGCAGCGTTAGCAGCTATTTTGATGAAATTGTCTTGCGCTGTTATGAGCGCTACGAAAAACAATTGTTAGCCAATAATGC
CCTCGATTTTGATGATTTGCTGCTCAAAACCGTTAATTTATTCGAATATCATCCCGATGTGCTAGCGCGGTATGCCGAAC
GTTATGTGCATGTCATGGTCGATGAAGTGCAAGATACCAATCGGGTGCAATTTTCGTTGATCAATCAAGTTGGTGCAGGC
CATAACAACTACTTTTTGATCGGCGATATTCAGCAATCAATTTATGCTTGGCGTGGGGCGAGGTTGGCGAATGTGCGCGA
ATTTGAAGAAGCCCACCCTGATGTGCAAATTATTCCACTGGAGCAAAATTACCGTTCCACCCAACCAATTCTTGATGTCG
CCCAATCGATCATCGATGCGGCCTATGATCGTCGCCATACCACCAAAATCTGGACTGATCAGCAGGATGGCGAGTTGGTT
TCGTTGGTCGAGGCCTATGATCACAATGAAGAAGCGCGTTGGGTGGCCGATGAAATTATGCGTATTCGCGGGCGTGAAGG
CCGTTCGCTCGACGATTTTGCTGTGATGTATCGCACCAACGCCCAATCCCGTGCCTTTGAAGAGGCCATGATCAGCCGCA
ACCTGCGCTATCGATTGGTCGGTGGCACGCGCTTCTACGAACGCAAAGAAATCAAAGATGTTGTGGCCTACCTACGAATT
ATCCACAATCCCCACGATGAAGTTAGTTTGCTACGGGTGATTAACGTGCCAGGCCGAAGCATCGGCGATCGCACTCAGCA
AGAGCTATTGCAATGGGCACGCAATCTCGATGTCTCAATTTGGGATGCCCTCGAACTGCTAGCTACTAACGAAGCCCAAA
GTCCGCCGATCAGCGGGCGAGCACGCAACGCCGTTGAGCAATTTCAAAAACTGGTAGCAAGCCTACGCGATCTGCGCCAC
GATTTGATGCTCGGCGAGTTGATTCAGCGCTTGCTTGAGCGGGTGCCATTGCAAGAGTTGCTGGTGGCAGAATATGGCGA
GGAAGAAGGCGCTGAGCGTTGGGAAAATATTCTCGAATTGCAAAACGTCAGTATGGAATATCTGGCCCTGCCGACCGAGG
ATCAATTGCCACGCTTTTTAGAAGAAGTGGCCTTGGTTAGCGATGTTGATAGCCTTGATTCCAACAAAGAGCGCGAGCCT
GGTGTGACCTTGATTACCTTGCACCAAGCCAAGGGTTTAGAGTATCCGGTAGTATTTTTGGCTGGCTTAGAAGAAGGCTT
GTTGCCGCACGGACGCTCAGTTGACGACCCCGAAAGTATCGAGGAAGAGCGTCGTTTGCTCTATGTTGGTACAACCCGCG
CCAAACAACGACTGTACATGCTCTATGCTTTCAAACGAGCAACTTGGGGCCGCACCGATATCACGATTCCTTCACGCTTT
TTGGGCGATATTCCCAAAGATTTGCTGCAGCGCACGCCCACCCGTGAAGTCAAACAAATGCCAGTTCATGCCGCAAGCCA
ATGGCAAAGCAGCACGCCGCAACGCACGCGAGGCACGCAACCAAGCACCAGCAGCATGTGGAGTGGCGCGAGTGGACCAG
TCCGGCCAAAACGCCCCGAACGTGAGCCAAGCGCCGCCAGTTATAGCGCTGGCGACAAAGTGCGCCATGCTAATTTCGGC
GAGGGCGTGGTGGTCAGTAGCAAAATGGTCGGCGACGACGAAGAAGTTACCGTGGCGTTTCCAGGCAAAGGCGTGAAAAA
GCTCTTGGCCGCCTTCGCCAAGCTCGAACGGGTTTAG

Upstream 100 bases:

>100_bases
GTGTCGCTCACCTGTTCCACCTCAGCGCCGGAACGGGTACAATAGAGAGAGCAACATACTAAACATCACGGTTATCGACC
GTCACATAAGGACTAGATCA

Downstream 100 bases:

>100_bases
CTGTGGTTTAGGAGGATGCGATGAATCAAACAACGTGGGATGCGGTTGATCAGTATATTTCCGATCGATTAGTCCAGGCT
GATCCGGTGTTGGATGCAGC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTHPLLIGLNAQQQRAVQAIHGPVLVLAGPGSGKTRVLTHRIAYLINEVGVRPYNILAVTFTNKAAREMRERIGNLIGES
RAHDVMMGTFHSICARWLRRDIQHLQRANDFVVYDADDQQRVMKQILRELDLSEKQYNPRSIHARISAAKNEMIGVAEFA
RSVSSYFDEIVLRCYERYEKQLLANNALDFDDLLLKTVNLFEYHPDVLARYAERYVHVMVDEVQDTNRVQFSLINQVGAG
HNNYFLIGDIQQSIYAWRGARLANVREFEEAHPDVQIIPLEQNYRSTQPILDVAQSIIDAAYDRRHTTKIWTDQQDGELV
SLVEAYDHNEEARWVADEIMRIRGREGRSLDDFAVMYRTNAQSRAFEEAMISRNLRYRLVGGTRFYERKEIKDVVAYLRI
IHNPHDEVSLLRVINVPGRSIGDRTQQELLQWARNLDVSIWDALELLATNEAQSPPISGRARNAVEQFQKLVASLRDLRH
DLMLGELIQRLLERVPLQELLVAEYGEEEGAERWENILELQNVSMEYLALPTEDQLPRFLEEVALVSDVDSLDSNKEREP
GVTLITLHQAKGLEYPVVFLAGLEEGLLPHGRSVDDPESIEEERRLLYVGTTRAKQRLYMLYAFKRATWGRTDITIPSRF
LGDIPKDLLQRTPTREVKQMPVHAASQWQSSTPQRTRGTQPSTSSMWSGASGPVRPKRPEREPSAASYSAGDKVRHANFG
EGVVVSSKMVGDDEEVTVAFPGKGVKKLLAAFAKLERV

Sequences:

>Translated_758_residues
MTHPLLIGLNAQQQRAVQAIHGPVLVLAGPGSGKTRVLTHRIAYLINEVGVRPYNILAVTFTNKAAREMRERIGNLIGES
RAHDVMMGTFHSICARWLRRDIQHLQRANDFVVYDADDQQRVMKQILRELDLSEKQYNPRSIHARISAAKNEMIGVAEFA
RSVSSYFDEIVLRCYERYEKQLLANNALDFDDLLLKTVNLFEYHPDVLARYAERYVHVMVDEVQDTNRVQFSLINQVGAG
HNNYFLIGDIQQSIYAWRGARLANVREFEEAHPDVQIIPLEQNYRSTQPILDVAQSIIDAAYDRRHTTKIWTDQQDGELV
SLVEAYDHNEEARWVADEIMRIRGREGRSLDDFAVMYRTNAQSRAFEEAMISRNLRYRLVGGTRFYERKEIKDVVAYLRI
IHNPHDEVSLLRVINVPGRSIGDRTQQELLQWARNLDVSIWDALELLATNEAQSPPISGRARNAVEQFQKLVASLRDLRH
DLMLGELIQRLLERVPLQELLVAEYGEEEGAERWENILELQNVSMEYLALPTEDQLPRFLEEVALVSDVDSLDSNKEREP
GVTLITLHQAKGLEYPVVFLAGLEEGLLPHGRSVDDPESIEEERRLLYVGTTRAKQRLYMLYAFKRATWGRTDITIPSRF
LGDIPKDLLQRTPTREVKQMPVHAASQWQSSTPQRTRGTQPSTSSMWSGASGPVRPKRPEREPSAASYSAGDKVRHANFG
EGVVVSSKMVGDDEEVTVAFPGKGVKKLLAAFAKLERV
>Mature_757_residues
THPLLIGLNAQQQRAVQAIHGPVLVLAGPGSGKTRVLTHRIAYLINEVGVRPYNILAVTFTNKAAREMRERIGNLIGESR
AHDVMMGTFHSICARWLRRDIQHLQRANDFVVYDADDQQRVMKQILRELDLSEKQYNPRSIHARISAAKNEMIGVAEFAR
SVSSYFDEIVLRCYERYEKQLLANNALDFDDLLLKTVNLFEYHPDVLARYAERYVHVMVDEVQDTNRVQFSLINQVGAGH
NNYFLIGDIQQSIYAWRGARLANVREFEEAHPDVQIIPLEQNYRSTQPILDVAQSIIDAAYDRRHTTKIWTDQQDGELVS
LVEAYDHNEEARWVADEIMRIRGREGRSLDDFAVMYRTNAQSRAFEEAMISRNLRYRLVGGTRFYERKEIKDVVAYLRII
HNPHDEVSLLRVINVPGRSIGDRTQQELLQWARNLDVSIWDALELLATNEAQSPPISGRARNAVEQFQKLVASLRDLRHD
LMLGELIQRLLERVPLQELLVAEYGEEEGAERWENILELQNVSMEYLALPTEDQLPRFLEEVALVSDVDSLDSNKEREPG
VTLITLHQAKGLEYPVVFLAGLEEGLLPHGRSVDDPESIEEERRLLYVGTTRAKQRLYMLYAFKRATWGRTDITIPSRFL
GDIPKDLLQRTPTREVKQMPVHAASQWQSSTPQRTRGTQPSTSSMWSGASGPVRPKRPEREPSAASYSAGDKVRHANFGE
GVVVSSKMVGDDEEVTVAFPGKGVKKLLAAFAKLERV

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=759, Percent_Identity=38.2081686429513, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI48994965, Length=657, Percent_Identity=35.3120243531202, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6322369, Length=751, Percent_Identity=28.2290279627164, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86536; Mature: 86405

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50007 PIPLC_X_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPLLIGLNAQQQRAVQAIHGPVLVLAGPGSGKTRVLTHRIAYLINEVGVRPYNILAVT
CCCCEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
FTNKAAREMRERIGNLIGESRAHDVMMGTFHSICARWLRRDIQHLQRANDFVVYDADDQQ
ECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
RVMKQILRELDLSEKQYNPRSIHARISAAKNEMIGVAEFARSVSSYFDEIVLRCYERYEK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLANNALDFDDLLLKTVNLFEYHPDVLARYAERYVHVMVDEVQDTNRVQFSLINQVGAG
HHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HNNYFLIGDIQQSIYAWRGARLANVREFEEAHPDVQIIPLEQNYRSTQPILDVAQSIIDA
CCCEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHH
AYDRRHTTKIWTDQQDGELVSLVEAYDHNEEARWVADEIMRIRGREGRSLDDFAVMYRTN
HHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHEEECC
AQSRAFEEAMISRNLRYRLVGGTRFYERKEIKDVVAYLRIIHNPHDEVSLLRVINVPGRS
CHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCC
IGDRTQQELLQWARNLDVSIWDALELLATNEAQSPPISGRARNAVEQFQKLVASLRDLRH
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLMLGELIQRLLERVPLQELLVAEYGEEEGAERWENILELQNVSMEYLALPTEDQLPRFL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHH
EEVALVSDVDSLDSNKEREPGVTLITLHQAKGLEYPVVFLAGLEEGLLPHGRSVDDPESI
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEECCHHCCCCCCCCCCCHHHH
EEERRLLYVGTTRAKQRLYMLYAFKRATWGRTDITIPSRFLGDIPKDLLQRTPTREVKQM
HHCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHHHC
PVHAASQWQSSTPQRTRGTQPSTSSMWSGASGPVRPKRPEREPSAASYSAGDKVRHANFG
CCHHHHHHCCCCCCHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGVVVSSKMVGDDEEVTVAFPGKGVKKLLAAFAKLERV
CCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
THPLLIGLNAQQQRAVQAIHGPVLVLAGPGSGKTRVLTHRIAYLINEVGVRPYNILAVT
CCCEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
FTNKAAREMRERIGNLIGESRAHDVMMGTFHSICARWLRRDIQHLQRANDFVVYDADDQQ
ECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
RVMKQILRELDLSEKQYNPRSIHARISAAKNEMIGVAEFARSVSSYFDEIVLRCYERYEK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLANNALDFDDLLLKTVNLFEYHPDVLARYAERYVHVMVDEVQDTNRVQFSLINQVGAG
HHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HNNYFLIGDIQQSIYAWRGARLANVREFEEAHPDVQIIPLEQNYRSTQPILDVAQSIIDA
CCCEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHH
AYDRRHTTKIWTDQQDGELVSLVEAYDHNEEARWVADEIMRIRGREGRSLDDFAVMYRTN
HHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHEEECC
AQSRAFEEAMISRNLRYRLVGGTRFYERKEIKDVVAYLRIIHNPHDEVSLLRVINVPGRS
CHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCC
IGDRTQQELLQWARNLDVSIWDALELLATNEAQSPPISGRARNAVEQFQKLVASLRDLRH
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLMLGELIQRLLERVPLQELLVAEYGEEEGAERWENILELQNVSMEYLALPTEDQLPRFL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHH
EEVALVSDVDSLDSNKEREPGVTLITLHQAKGLEYPVVFLAGLEEGLLPHGRSVDDPESI
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEECCHHCCCCCCCCCCCHHHH
EEERRLLYVGTTRAKQRLYMLYAFKRATWGRTDITIPSRFLGDIPKDLLQRTPTREVKQM
HHCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHHHC
PVHAASQWQSSTPQRTRGTQPSTSSMWSGASGPVRPKRPEREPSAASYSAGDKVRHANFG
CCHHHHHHCCCCCCHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGVVVSSKMVGDDEEVTVAFPGKGVKKLLAAFAKLERV
CCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]