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The map label for this gene is ydfA [H]

Identifier: 159901039

GI number: 159901039

Start: 5792672

End: 5793931

Strand: Reverse

Name: ydfA [H]

Synonym: Haur_4527

Alternate gene names: 159901039

Gene position: 5793931-5792672 (Counterclockwise)

Preceding gene: 159901040

Following gene: 159901038

Centisome position: 91.29

GC content: 50.32

Gene sequence:

>1260_bases
ATGCAACTGCTACTTGGTGGAACAATTTTTGGAGCCACGCTCTTAGGCGTGATGGTTCGTCCGCGCAATATTTCGGAAGC
TTGGGTCGCGTTACTTGGCGCAGTTGCCATGTTATTGGTCGGAATTTTGCCACTGAAGGCAATTTTGCCGACGCTTGCCC
GCGAATGGAATGTGTATGGTTTTTTTGCCGGTTTGATGCTGATTGCCTTTTTTGCCGAGCAAGCTGGGGTGTTTCAGGCC
TTGGCGTTGCAGGCAGCACGTTGGGCCAATGGCTCGGCGCAGCGGCTCTATTTGGCGGTCTTTTTGGTGGGCACGCTGAT
TACAGCGGTGCTCTCCAACGATGCGACAGCCTTGATTTTAACTCCAGTGGTTTGGACGTTGGCCAGCCGTTTGCGCTTGC
CAGCCTTGCCATTTATGTTTGCCTGCACCTTTATTGCCGATACTGCTTCGGCATTGCTGCCCGTTTCCAATCCGATTAAT
ATTTTGGTGCTAACCCGCTTTAATCGTGAGCTGTTGGAATATTGGGCCTATTTGTTGGTTCCGTCACTGGTGTGTATTGG
CTGGAATATTGGGCTATTTGCTTGGCTGTTTCGGCGCGATTTGCAGGGCAGCTACGATTTGGCACTGCTTGATGATTTAA
CTATCGCCAACCCACGCCTTTATCGGACAACCCTTGTTGGCTTGGGCAGTATTGCTGTGGCCTATGTAGCTGGCTCGTTG
TGGCACGTGCCATTGGCCTTTGTGGCGTTGGCTGGAGCCGCTCTCTTAGCGGCAATTAGCTGGTGGAATGGCACGTTTAA
GCCCAAACAAGCCTTGCACGAATTATCTCCAGCCTTGTTTGGCTTTATTAGCGGCATGTTTTTGGTGGTACGGGCGATTG
AGCAATTAGGCTGGACTGAACGCTTTGGCGCGAGTTTGTTACAGGGCAGCGGCGCGAGTTTGGGCAATATTGCGCGGGTA
ATTTTCGGTAGTGCACTTGGCTCGAACATGATCAACAATGTGCCGATGACCTTGGTGATGACCTCGACGCTTGAACATTT
GCCTAGCACACCTGAGCCTGCGTTGATTTATGCCACCATTTTTGGGGCTGACCTTGGGCCAAATTTAACAATTGTTGGCT
CGTTGGCTACAATGCTGTGGTTGGTGATTTTACGGCGCAAAGGTCTGGAAATTAGTGCCAAACAATACTTTAAATTGGGC
TTGCTGTTTGTGCTACCATCCTTATTAATTGGTACATTTTGGATGTGGCTGATGGCATGA

Upstream 100 bases:

>100_bases
CATCAATTACTCCGCTAGCCAACTAGAGCCATCCATGCTTCGCGGTTAAAAATCTTGCCCCTGAACCCCGACAACTAACC
CCTCATTTAATAGGAAGATC

Downstream 100 bases:

>100_bases
ATTATTACTTCTTTGACCAATTAACGCGCTGGCTGGAACGGCGGGGCATGGATTATACCCGTCCGATGCAAACCAACCCG
CAGGCCGCCGATGTACATCT

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQLLLGGTIFGATLLGVMVRPRNISEAWVALLGAVAMLLVGILPLKAILPTLAREWNVYGFFAGLMLIAFFAEQAGVFQA
LALQAARWANGSAQRLYLAVFLVGTLITAVLSNDATALILTPVVWTLASRLRLPALPFMFACTFIADTASALLPVSNPIN
ILVLTRFNRELLEYWAYLLVPSLVCIGWNIGLFAWLFRRDLQGSYDLALLDDLTIANPRLYRTTLVGLGSIAVAYVAGSL
WHVPLAFVALAGAALLAAISWWNGTFKPKQALHELSPALFGFISGMFLVVRAIEQLGWTERFGASLLQGSGASLGNIARV
IFGSALGSNMINNVPMTLVMTSTLEHLPSTPEPALIYATIFGADLGPNLTIVGSLATMLWLVILRRKGLEISAKQYFKLG
LLFVLPSLLIGTFWMWLMA

Sequences:

>Translated_419_residues
MQLLLGGTIFGATLLGVMVRPRNISEAWVALLGAVAMLLVGILPLKAILPTLAREWNVYGFFAGLMLIAFFAEQAGVFQA
LALQAARWANGSAQRLYLAVFLVGTLITAVLSNDATALILTPVVWTLASRLRLPALPFMFACTFIADTASALLPVSNPIN
ILVLTRFNRELLEYWAYLLVPSLVCIGWNIGLFAWLFRRDLQGSYDLALLDDLTIANPRLYRTTLVGLGSIAVAYVAGSL
WHVPLAFVALAGAALLAAISWWNGTFKPKQALHELSPALFGFISGMFLVVRAIEQLGWTERFGASLLQGSGASLGNIARV
IFGSALGSNMINNVPMTLVMTSTLEHLPSTPEPALIYATIFGADLGPNLTIVGSLATMLWLVILRRKGLEISAKQYFKLG
LLFVLPSLLIGTFWMWLMA
>Mature_419_residues
MQLLLGGTIFGATLLGVMVRPRNISEAWVALLGAVAMLLVGILPLKAILPTLAREWNVYGFFAGLMLIAFFAEQAGVFQA
LALQAARWANGSAQRLYLAVFLVGTLITAVLSNDATALILTPVVWTLASRLRLPALPFMFACTFIADTASALLPVSNPIN
ILVLTRFNRELLEYWAYLLVPSLVCIGWNIGLFAWLFRRDLQGSYDLALLDDLTIANPRLYRTTLVGLGSIAVAYVAGSL
WHVPLAFVALAGAALLAAISWWNGTFKPKQALHELSPALFGFISGMFLVVRAIEQLGWTERFGASLLQGSGASLGNIARV
IFGSALGSNMINNVPMTLVMTSTLEHLPSTPEPALIYATIFGADLGPNLTIVGSLATMLWLVILRRKGLEISAKQYFKLG
LLFVLPSLLIGTFWMWLMA

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=420, Percent_Identity=31.4285714285714, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45427; Mature: 45427

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLLGGTIFGATLLGVMVRPRNISEAWVALLGAVAMLLVGILPLKAILPTLAREWNVYG
CCEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FFAGLMLIAFFAEQAGVFQALALQAARWANGSAQRLYLAVFLVGTLITAVLSNDATALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TPVVWTLASRLRLPALPFMFACTFIADTASALLPVSNPINILVLTRFNRELLEYWAYLLV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHH
PSLVCIGWNIGLFAWLFRRDLQGSYDLALLDDLTIANPRLYRTTLVGLGSIAVAYVAGSL
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WHVPLAFVALAGAALLAAISWWNGTFKPKQALHELSPALFGFISGMFLVVRAIEQLGWTE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RFGASLLQGSGASLGNIARVIFGSALGSNMINNVPMTLVMTSTLEHLPSTPEPALIYATI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
FGADLGPNLTIVGSLATMLWLVILRRKGLEISAKQYFKLGLLFVLPSLLIGTFWMWLMA
ECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQLLLGGTIFGATLLGVMVRPRNISEAWVALLGAVAMLLVGILPLKAILPTLAREWNVYG
CCEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FFAGLMLIAFFAEQAGVFQALALQAARWANGSAQRLYLAVFLVGTLITAVLSNDATALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TPVVWTLASRLRLPALPFMFACTFIADTASALLPVSNPINILVLTRFNRELLEYWAYLLV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHH
PSLVCIGWNIGLFAWLFRRDLQGSYDLALLDDLTIANPRLYRTTLVGLGSIAVAYVAGSL
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WHVPLAFVALAGAALLAAISWWNGTFKPKQALHELSPALFGFISGMFLVVRAIEQLGWTE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RFGASLLQGSGASLGNIARVIFGSALGSNMINNVPMTLVMTSTLEHLPSTPEPALIYATI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
FGADLGPNLTIVGSLATMLWLVILRRKGLEISAKQYFKLGLLFVLPSLLIGTFWMWLMA
ECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]