Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 159900973

GI number: 159900973

Start: 5702618

End: 5703979

Strand: Reverse

Name: purD [H]

Synonym: Haur_4460

Alternate gene names: 159900973

Gene position: 5703979-5702618 (Counterclockwise)

Preceding gene: 159900974

Following gene: 159900972

Centisome position: 89.87

GC content: 52.94

Gene sequence:

>1362_bases
ATGAAAGTACTTGTGCTTGGCAAAAATGGCGCAGCCCATGCCTATGTTTGGAAGTTGTTCAATAGCCAACGGATCGATAG
TTTAGCAACCATGCCAGGCAATGGTGGTACAAGTTTGCTTGTGCCCAATGCTGAGCACATTGAAAAAATTAACGAACTGG
CCAAATGGGCCTACGACGAACACTTCCAAGTAATTATTCCAGCCGAAGGCAGCTATCTCAGTGCGGGTTTGGCTGATGCC
GCCGCTGCCTATAAAATTCCGGTTTGTGGGCCGCCCAAAAATTCGGCGGTGCTGGAGCGTTCGCGGCTTTGGCTCAAAGA
ATTTCTCAATCGCCACCAAATTCCGACCTCAACTGGCCGTGCCTTGGGCGATTTAGCCACGGCAGAACGCTATGTGGCCT
CGCAACCTATGCCCTTGGTATTATGGGGCGATTATCCTTCAGAATATGATGGAGCCTATACCGACCGCGCCGAAGCCTTA
GCTGCCTTGGCGGGCATTTTTGCCTTGCCGCCGCGCGAAGGCCATTATCGTGGCGTGGTGATTGAGCAGCCAGCACTTGG
CCCAACCGTGGCGCTTTCAGCAATTACCGATGGCACAACCCTGATTCCAATGCAAGCAGTTCGAATGTATGATCGACTTG
AGGATAATGATCAGGGCTGGGCGGCTGAAGGCATGGGGGCGCATACCACGCCTAGCGGAATTCTCAAACAACTTGGCGAT
TATCTCAGCGCTGCCATTCTTGAGCCAATTCGTGCTGCTCTCTTGCGCGACAAAATGCCTTGGTGGGGCATTTTAGGGGT
CGATTGTTCGATTACCGCCAGCGGGCCACAAGTGCTGCGCATTCGCACAACCTTGAGTGACCCTGAGGCCCAAGTCTTAT
TACCCTTGCTCGAAGACGATTTATTTCCCTTTTTGGTAGCGGCCAACAATCGCAGTTTACTCAATGTACCAGCTTTGCGT
TGGCGCAATGAGGCGAGCGTTGGCGTAACCATGGTCGCCAACGGCTACCCGCATAGTTTTAGCACTGGGATGGCCATCGA
TGGGATGCCGGAGCTGGAGCCGGGGGTTGTTGCATTTCAAAACGAAACTGCCAATCCCAATGGCTTGCGTTATGTTTCCG
ACGAAATGCATATGCCTGAAAAACGTGGCCTATTCAGCGGGGTCAGCGATGCCCTGAGTGATGCAATTCTGGTCCGCTCA
ATGGGTCCAACCCGTCTATCAAGCCAAGCAGGGCGAATCGTTACTGTCACCGCCCAAGCTTCAAGTTTGGCAGCAGCCCA
CGAAAAAGCCTATAACAACCTCAAACGGCTCCGGATCGCTTCAGCGGTTTATCGTAACGATATCGGGCTGCGCGAGCTAT
AG

Upstream 100 bases:

>100_bases
CTCGCGAAGGCTCAGCAGCCTAACCTAAAACCGAGTAAATTAATTATTACGCGATTGCTGTTAACCCAAATAGCAGCAAT
CGCGCTAGCACCTCAGGCCT

Downstream 100 bases:

>100_bases
CAGCGCTGGAATATCGGTCTTTGGTCGGTAGTATGTGCCTAAACTACCGATTATGCTTGGTTTAATGCTGCAATTGAGGG
AAGACGAATATGCTACTCCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKVLVLGKNGAAHAYVWKLFNSQRIDSLATMPGNGGTSLLVPNAEHIEKINELAKWAYDEHFQVIIPAEGSYLSAGLADA
AAAYKIPVCGPPKNSAVLERSRLWLKEFLNRHQIPTSTGRALGDLATAERYVASQPMPLVLWGDYPSEYDGAYTDRAEAL
AALAGIFALPPREGHYRGVVIEQPALGPTVALSAITDGTTLIPMQAVRMYDRLEDNDQGWAAEGMGAHTTPSGILKQLGD
YLSAAILEPIRAALLRDKMPWWGILGVDCSITASGPQVLRIRTTLSDPEAQVLLPLLEDDLFPFLVAANNRSLLNVPALR
WRNEASVGVTMVANGYPHSFSTGMAIDGMPELEPGVVAFQNETANPNGLRYVSDEMHMPEKRGLFSGVSDALSDAILVRS
MGPTRLSSQAGRIVTVTAQASSLAAAHEKAYNNLKRLRIASAVYRNDIGLREL

Sequences:

>Translated_453_residues
MKVLVLGKNGAAHAYVWKLFNSQRIDSLATMPGNGGTSLLVPNAEHIEKINELAKWAYDEHFQVIIPAEGSYLSAGLADA
AAAYKIPVCGPPKNSAVLERSRLWLKEFLNRHQIPTSTGRALGDLATAERYVASQPMPLVLWGDYPSEYDGAYTDRAEAL
AALAGIFALPPREGHYRGVVIEQPALGPTVALSAITDGTTLIPMQAVRMYDRLEDNDQGWAAEGMGAHTTPSGILKQLGD
YLSAAILEPIRAALLRDKMPWWGILGVDCSITASGPQVLRIRTTLSDPEAQVLLPLLEDDLFPFLVAANNRSLLNVPALR
WRNEASVGVTMVANGYPHSFSTGMAIDGMPELEPGVVAFQNETANPNGLRYVSDEMHMPEKRGLFSGVSDALSDAILVRS
MGPTRLSSQAGRIVTVTAQASSLAAAHEKAYNNLKRLRIASAVYRNDIGLREL
>Mature_453_residues
MKVLVLGKNGAAHAYVWKLFNSQRIDSLATMPGNGGTSLLVPNAEHIEKINELAKWAYDEHFQVIIPAEGSYLSAGLADA
AAAYKIPVCGPPKNSAVLERSRLWLKEFLNRHQIPTSTGRALGDLATAERYVASQPMPLVLWGDYPSEYDGAYTDRAEAL
AALAGIFALPPREGHYRGVVIEQPALGPTVALSAITDGTTLIPMQAVRMYDRLEDNDQGWAAEGMGAHTTPSGILKQLGD
YLSAAILEPIRAALLRDKMPWWGILGVDCSITASGPQVLRIRTTLSDPEAQVLLPLLEDDLFPFLVAANNRSLLNVPALR
WRNEASVGVTMVANGYPHSFSTGMAIDGMPELEPGVVAFQNETANPNGLRYVSDEMHMPEKRGLFSGVSDALSDAILVRS
MGPTRLSSQAGRIVTVTAQASSLAAAHEKAYNNLKRLRIASAVYRNDIGLREL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=472, Percent_Identity=25.8474576271186, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI209869995, Length=472, Percent_Identity=25.8474576271186, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI209869993, Length=472, Percent_Identity=25.8474576271186, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI28416899, Length=472, Percent_Identity=25.8474576271186, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1790438, Length=460, Percent_Identity=26.7391304347826, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17567511, Length=464, Percent_Identity=26.0775862068966, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321203, Length=484, Percent_Identity=25.2066115702479, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI62471619, Length=373, Percent_Identity=28.9544235924933, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24582400, Length=373, Percent_Identity=28.9544235924933, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 48994; Mature: 48994

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVLGKNGAAHAYVWKLFNSQRIDSLATMPGNGGTSLLVPNAEHIEKINELAKWAYDE
CEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCCC
HFQVIIPAEGSYLSAGLADAAAAYKIPVCGPPKNSAVLERSRLWLKEFLNRHQIPTSTGR
CEEEEEECCCCEECCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
ALGDLATAERYVASQPMPLVLWGDYPSEYDGAYTDRAEALAALAGIFALPPREGHYRGVV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IEQPALGPTVALSAITDGTTLIPMQAVRMYDRLEDNDQGWAAEGMGAHTTPSGILKQLGD
EECCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YLSAAILEPIRAALLRDKMPWWGILGVDCSITASGPQVLRIRTTLSDPEAQVLLPLLEDD
HHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCEEEEEEECCCCCCHHEEHHHHHCC
LFPFLVAANNRSLLNVPALRWRNEASVGVTMVANGYPHSFSTGMAIDGMPELEPGVVAFQ
CCEEEEEECCCCEEECCCHHCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCEEEEE
NETANPNGLRYVSDEMHMPEKRGLFSGVSDALSDAILVRSMGPTRLSSQAGRIVTVTAQA
CCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCEEEEEECH
SSLAAAHEKAYNNLKRLRIASAVYRNDIGLREL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVLVLGKNGAAHAYVWKLFNSQRIDSLATMPGNGGTSLLVPNAEHIEKINELAKWAYDE
CEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCCC
HFQVIIPAEGSYLSAGLADAAAAYKIPVCGPPKNSAVLERSRLWLKEFLNRHQIPTSTGR
CEEEEEECCCCEECCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
ALGDLATAERYVASQPMPLVLWGDYPSEYDGAYTDRAEALAALAGIFALPPREGHYRGVV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IEQPALGPTVALSAITDGTTLIPMQAVRMYDRLEDNDQGWAAEGMGAHTTPSGILKQLGD
EECCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YLSAAILEPIRAALLRDKMPWWGILGVDCSITASGPQVLRIRTTLSDPEAQVLLPLLEDD
HHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCEEEEEEECCCCCCHHEEHHHHHCC
LFPFLVAANNRSLLNVPALRWRNEASVGVTMVANGYPHSFSTGMAIDGMPELEPGVVAFQ
CCEEEEEECCCCEEECCCHHCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCEEEEE
NETANPNGLRYVSDEMHMPEKRGLFSGVSDALSDAILVRSMGPTRLSSQAGRIVTVTAQA
CCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCEEEEEECH
SSLAAAHEKAYNNLKRLRIASAVYRNDIGLREL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA