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The map label for this gene is ilvD [H]

Identifier: 159900963

GI number: 159900963

Start: 5692652

End: 5694337

Strand: Reverse

Name: ilvD [H]

Synonym: Haur_4450

Alternate gene names: 159900963

Gene position: 5694337-5692652 (Counterclockwise)

Preceding gene: 159900964

Following gene: 159900962

Centisome position: 89.72

GC content: 55.87

Gene sequence:

>1686_bases
ATGCGTTCAGATACGATCAAGCGGGGCTTTGCACGCGCTCCACATCGTTCACTCCTACGTGCGACGGGCCAAATTCAAGA
TGATAGCGACTTTCAAAAGCCGTTCGTCGCGATCTGTAATTCGTATATCGATATTATTCCTGGCCACGTTCACTTGCATG
AGTTTGCCAAAATCGTCAAAGATGCAGTACGGGCAGCAGGCGGCATTCCCTTTGAATTCAACACGATTGGGGTTGATGAT
GGCATCGTGATGGGCCACGAAGGTATGCGCTATTCCTTGCCATCGCGCGAGTTGATCGCCGATTGTGTTGAAACGGTGGC
AGCAGCCCACTGTTTCGATGCGATGATCTGTATCCCCAACTGCGATAAAATCGTGCCTGGCATGCTGATGGGCGCTGCCC
GCGTCAACATCCCCACCGTTTTCGTATCGGGCGGGCCAATGCAGGCTGGCCGCGATAAAGATGGCAATAAAGTTGACTTG
ATCAGCGTGTTCGAGGGCGTTGGTCAACATGCTTCTGGCCGCATCAGTGATGAACGGCTGCTGGATCTGGAGCGCAACGG
CTGCCCGACCTGCGGCTCCTGCTCCGGCATGTTCACCGCCAATTCGATGAATTGTCTGTGTGAAGCACTGGGAATCGCCC
TGCCATACAACGGTTCATTACTGGCCACCGACCCTGCCCGCCACGAGCTAGCCCGCACCGCCGCCACCAAAGTCCTCGAA
TTGCTCCGCCAGAATATTTCCTTCAGCGATATTGTCACTCCCGAATCAATTGATAACGCGATGGCGCTCGACGTAGCCAT
GGGCGGCTCGACCAACACCATCTTGCACGTTTTGGCCTTGGCGCGTGAAGCAGGCTTGGATTACCCAATCAGCCGCTTTA
ACGAGGTTGCAGCTCGCGTACCACACTTGGCCAAAGTTAGCCCAGCCTGGGATGGCACTCGCCAATGGCATATGGAAGAT
GTGCATCGCGCTGGTGGCGTACCAGCAATTATGGCCGAATTGGCCAAAAAGCCCGATGCCCTACACTTGAATGTGCCAAC
CGTGACAGGCCAAACCTTGGGTGAACAATTGCAAGGCATCGCCAACGAAAACCCTGAATGCATCCGCCCAATCGAACATC
CCCATTCAGCCCAAGGCGGTTTGTGTATTTTGTTTGGCAACTTGGCTCCTGAAGGCTCGGTGATCAAAATTGGTGCAGTT
GATCAACATCAAATGACATTTAGCGGCCCGGCCCGTTGTTTCCCCAGCGAAGAAGCCGCCACCTACGCCGCCCGCGCTGG
CGAAATTCAAGCTGGCGATGTGGTGGTAGTGCGCTACGAAGGCCCACGCGGTGGCCCAGGTATGCGCGAAATGCTGGCCT
TAACCTCGTTGCTCAAAGGCATGCCCTTGGGCGAGCAAGTGGCCTTATTGACCGATGGTCGCTTCAGCGGCGGCACACGC
GGCCTGTGTATTGGCCATATCTCCCCCGAAGCCGCCGAGGGTGGCCCAATTGGCCTGATCGAAAATGGCGACATCATTCA
CATCGATTTAGCAAATCGCCTGTTGGCAGTTGACCTCAGCGAAACGCAATTTGCCGAACGCCGCGCTGCGTGGCAAGCCC
CAGAACGCAAACATCAACGCGGCTGGTTGGCACGCTACACCCGTTTGGTGACGAACGCCAGCAATGGCGCGGTGTTGGAA
GCATAG

Upstream 100 bases:

>100_bases
CAAAATCTTGCGATCTGCGGCCAATCCCACTGGAGCATTGGCTCAATCGCCTTTCAACGATGGCCTGTCCAGCCATCAGT
TCTCTATGCGGGGAGCATCT

Downstream 100 bases:

>100_bases
ATCTGCAACTTATTGACATTAAACCTTACGCAAGGCGAGGTATACCGATGAAAACGCTAACTGGTGCTCAAATGATGTGT
GAAGCGTTGATAGCCGAAGG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRSDTIKRGFARAPHRSLLRATGQIQDDSDFQKPFVAICNSYIDIIPGHVHLHEFAKIVKDAVRAAGGIPFEFNTIGVDD
GIVMGHEGMRYSLPSRELIADCVETVAAAHCFDAMICIPNCDKIVPGMLMGAARVNIPTVFVSGGPMQAGRDKDGNKVDL
ISVFEGVGQHASGRISDERLLDLERNGCPTCGSCSGMFTANSMNCLCEALGIALPYNGSLLATDPARHELARTAATKVLE
LLRQNISFSDIVTPESIDNAMALDVAMGGSTNTILHVLALAREAGLDYPISRFNEVAARVPHLAKVSPAWDGTRQWHMED
VHRAGGVPAIMAELAKKPDALHLNVPTVTGQTLGEQLQGIANENPECIRPIEHPHSAQGGLCILFGNLAPEGSVIKIGAV
DQHQMTFSGPARCFPSEEAATYAARAGEIQAGDVVVVRYEGPRGGPGMREMLALTSLLKGMPLGEQVALLTDGRFSGGTR
GLCIGHISPEAAEGGPIGLIENGDIIHIDLANRLLAVDLSETQFAERRAAWQAPERKHQRGWLARYTRLVTNASNGAVLE
A

Sequences:

>Translated_561_residues
MRSDTIKRGFARAPHRSLLRATGQIQDDSDFQKPFVAICNSYIDIIPGHVHLHEFAKIVKDAVRAAGGIPFEFNTIGVDD
GIVMGHEGMRYSLPSRELIADCVETVAAAHCFDAMICIPNCDKIVPGMLMGAARVNIPTVFVSGGPMQAGRDKDGNKVDL
ISVFEGVGQHASGRISDERLLDLERNGCPTCGSCSGMFTANSMNCLCEALGIALPYNGSLLATDPARHELARTAATKVLE
LLRQNISFSDIVTPESIDNAMALDVAMGGSTNTILHVLALAREAGLDYPISRFNEVAARVPHLAKVSPAWDGTRQWHMED
VHRAGGVPAIMAELAKKPDALHLNVPTVTGQTLGEQLQGIANENPECIRPIEHPHSAQGGLCILFGNLAPEGSVIKIGAV
DQHQMTFSGPARCFPSEEAATYAARAGEIQAGDVVVVRYEGPRGGPGMREMLALTSLLKGMPLGEQVALLTDGRFSGGTR
GLCIGHISPEAAEGGPIGLIENGDIIHIDLANRLLAVDLSETQFAERRAAWQAPERKHQRGWLARYTRLVTNASNGAVLE
A
>Mature_561_residues
MRSDTIKRGFARAPHRSLLRATGQIQDDSDFQKPFVAICNSYIDIIPGHVHLHEFAKIVKDAVRAAGGIPFEFNTIGVDD
GIVMGHEGMRYSLPSRELIADCVETVAAAHCFDAMICIPNCDKIVPGMLMGAARVNIPTVFVSGGPMQAGRDKDGNKVDL
ISVFEGVGQHASGRISDERLLDLERNGCPTCGSCSGMFTANSMNCLCEALGIALPYNGSLLATDPARHELARTAATKVLE
LLRQNISFSDIVTPESIDNAMALDVAMGGSTNTILHVLALAREAGLDYPISRFNEVAARVPHLAKVSPAWDGTRQWHMED
VHRAGGVPAIMAELAKKPDALHLNVPTVTGQTLGEQLQGIANENPECIRPIEHPHSAQGGLCILFGNLAPEGSVIKIGAV
DQHQMTFSGPARCFPSEEAATYAARAGEIQAGDVVVVRYEGPRGGPGMREMLALTSLLKGMPLGEQVALLTDGRFSGGTR
GLCIGHISPEAAEGGPIGLIENGDIIHIDLANRLLAVDLSETQFAERRAAWQAPERKHQRGWLARYTRLVTNASNGAVLE
A

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=47.3770491803279, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI2367371, Length=526, Percent_Identity=34.6007604562738, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1786464, Length=496, Percent_Identity=35.2822580645161, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788157, Length=524, Percent_Identity=29.9618320610687, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=555, Percent_Identity=40.9009009009009, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59999; Mature: 59999

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2 ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDTIKRGFARAPHRSLLRATGQIQDDSDFQKPFVAICNSYIDIIPGHVHLHEFAKIVK
CCCCHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
DAVRAAGGIPFEFNTIGVDDGIVMGHEGMRYSLPSRELIADCVETVAAAHCFDAMICIPN
HHHHHHCCCCEEEEEECCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHEEECCC
CDKIVPGMLMGAARVNIPTVFVSGGPMQAGRDKDGNKVDLISVFEGVGQHASGRISDERL
CCHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCHHHH
LDLERNGCPTCGSCSGMFTANSMNCLCEALGIALPYNGSLLATDPARHELARTAATKVLE
HCCCCCCCCCCCCCCCCEECCHHHHHHHHHCEECCCCCCEEECCHHHHHHHHHHHHHHHH
LLRQNISFSDIVTPESIDNAMALDVAMGGSTNTILHVLALAREAGLDYPISRFNEVAARV
HHHHCCCHHHCCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PHLAKVSPAWDGTRQWHMEDVHRAGGVPAIMAELAKKPDALHLNVPTVTGQTLGEQLQGI
CCHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ANENPECIRPIEHPHSAQGGLCILFGNLAPEGSVIKIGAVDQHQMTFSGPARCFPSEEAA
CCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHH
TYAARAGEIQAGDVVVVRYEGPRGGPGMREMLALTSLLKGMPLGEQVALLTDGRFSGGTR
HHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GLCIGHISPEAAEGGPIGLIENGDIIHIDLANRLLAVDLSETQFAERRAAWQAPERKHQR
CEEEECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHCCCHHHHHH
GWLARYTRLVTNASNGAVLEA
HHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MRSDTIKRGFARAPHRSLLRATGQIQDDSDFQKPFVAICNSYIDIIPGHVHLHEFAKIVK
CCCCHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
DAVRAAGGIPFEFNTIGVDDGIVMGHEGMRYSLPSRELIADCVETVAAAHCFDAMICIPN
HHHHHHCCCCEEEEEECCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHEEECCC
CDKIVPGMLMGAARVNIPTVFVSGGPMQAGRDKDGNKVDLISVFEGVGQHASGRISDERL
CCHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCHHHH
LDLERNGCPTCGSCSGMFTANSMNCLCEALGIALPYNGSLLATDPARHELARTAATKVLE
HCCCCCCCCCCCCCCCCEECCHHHHHHHHHCEECCCCCCEEECCHHHHHHHHHHHHHHHH
LLRQNISFSDIVTPESIDNAMALDVAMGGSTNTILHVLALAREAGLDYPISRFNEVAARV
HHHHCCCHHHCCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PHLAKVSPAWDGTRQWHMEDVHRAGGVPAIMAELAKKPDALHLNVPTVTGQTLGEQLQGI
CCHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ANENPECIRPIEHPHSAQGGLCILFGNLAPEGSVIKIGAVDQHQMTFSGPARCFPSEEAA
CCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHH
TYAARAGEIQAGDVVVVRYEGPRGGPGMREMLALTSLLKGMPLGEQVALLTDGRFSGGTR
HHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GLCIGHISPEAAEGGPIGLIENGDIIHIDLANRLLAVDLSETQFAERRAAWQAPERKHQR
CEEEECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHCCCHHHHHH
GWLARYTRLVTNASNGAVLEA
HHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA