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The map label for this gene is ilvB1 [H]

Identifier: 159900962

GI number: 159900962

Start: 5690892

End: 5692604

Strand: Reverse

Name: ilvB1 [H]

Synonym: Haur_4449

Alternate gene names: 159900962

Gene position: 5692604-5690892 (Counterclockwise)

Preceding gene: 159900963

Following gene: 159900961

Centisome position: 89.7

GC content: 52.6

Gene sequence:

>1713_bases
ATGAAAACGCTAACTGGTGCTCAAATGATGTGTGAAGCGTTGATAGCCGAAGGCGTTGATGTGATGTTTGGCTATCCAGG
TGGGGCGATTATGCCGTTCTACCATGCGCTGCCCGAATATCCCCAATTGCGCCATATTTTGGTGCGCCACGAACAAGCGG
CTGGCCATGCCGCCGAAGGCTACGCCCGTACCAGCGGCAAAGTTGGGGTTTGTGTCGCCACCAGCGGCCCGGGCGCAACC
AACTTGGTCACGGCAATTGCCGATGCCATGATGGATTCAATCCCGATCGTAGCGATCACTGGGCAAGTTGTCACCTCGTT
AATCGGCAAAGATGGCTTTCAAGAAACCGATGTGACGGGCATCACCCAGCCTATCACCAAGCACAATTATTTGGTGACCG
ATGTGCGCGATTTGCCCCGTGTGTTCAAAGAAGCGTTTCATATTGCCCGAACTGGCCGCCCCGGCCCAGTCTTGATCGAC
GTTGCCAAAGATACCCAAAACGCTCGGGGCGTATTCGAATATCCAGAATCGGTCGATTTACCAGGCTACAAGCCCAACTA
CGATGGCAATCGCCGCCAAATCAAAGAAGCTGCCAATTTGCTGAATCAAGCCAAAAAGCCCTTGATTATGGCAGGCCATG
GCATTTTGCTGGGTCACGCGAGCGTCGAATTACGCGAATTTGCCGAAAAAACTGGTATTCCGGTGATCACCACATTGTTG
GGCATTAGCTGTATTCCCGAAGATCATCCGTTATCGTTGGGCATGCCTGGCATGCACGGTTGGGTTCATGTCAATCGCGC
CATCCAAGAATGCGATGTGTTATTTACCATCGGCGCACGCTTCGACGACCGTGTAACTGGCAAATTAGATACCTTTGCCC
GCAACGCCAAAATCATTCATGTTGATATTGATCCGGCTGAAGTTGGCAAAAATGTGATTACTGCTGTGCCAATTGTCGGC
GATGCCAAGCGTGTGCTAGAAAACTTGACCGAAATTGTCAACGCCAACGAGCATAGCGAATGGCTCAACCATATTCGTGA
TATTCAGGCCACGCGCTCCAAGCGCCAACGCGACCCAGAAAGTGGCATGTTCACGCCACACCATGTTTATGCCGAATTCA
CCCGCATTATGCAAGGCGAGTTTCGGGTTTGCACTGACGTGGGCCAGCACCAAATGTGGGCGGCTCAGTTGATCGAATAT
ATGAAGCCCCATACCCACATCACCTCTGGCGGTTTGGGAACCATGGGTTTTGCCTTGCCAGCGGCGATGGGCGTACAAGT
CGCTTGCCCCAACGAAGAAGTTTGGGCAATTGCTGGCGACGGCGGCTTCCAGATGAATTTGCAAGAGCTAGCCACGGTGG
TGCAAGAAAGCCTGCCCTTGAAGATTTGTATCATCAACAACGGCTACTTGGGCATGGTGCGCCAATGGCAAGAGCTTTTC
CACGAACGCCGCTATTCGGCAACGCCAATGTGGAGTCCCGATTTTATCAAGTTGGCTGATGCTTATGGCATTCCTGGGGT
GCGGGTCGAACGCGCCGAAGATGTGGCCGAGGCAATTCAGCAAGCCCGCTCAACCAATGGCCCATTCCTGATTGAATTTG
TGGTTGAACGCGAAGTCAACGTCTATCCGATGGTGGCTCCAGGCGCGAGCATCAGCGATATGTTCGAAGATCCCAACGAA
GTCGAAATCGAAGACGTTGTGGCAGTGAAATAG

Upstream 100 bases:

>100_bases
GCTACACCCGTTTGGTGACGAACGCCAGCAATGGCGCGGTGTTGGAAGCATAGATCTGCAACTTATTGACATTAAACCTT
ACGCAAGGCGAGGTATACCG

Downstream 100 bases:

>100_bases
GCTCACAGGAGATTTTTGCAATGAAACATACCTTAGTTGCTTTATTACGTGACCAACCTGGGGTGCTGAACCGCTCAGTG
AGCCTGTTTCGCCGCCGTGG

Product: acetolactate synthase large subunit, biosynthetic type

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKTLTGAQMMCEALIAEGVDVMFGYPGGAIMPFYHALPEYPQLRHILVRHEQAAGHAAEGYARTSGKVGVCVATSGPGAT
NLVTAIADAMMDSIPIVAITGQVVTSLIGKDGFQETDVTGITQPITKHNYLVTDVRDLPRVFKEAFHIARTGRPGPVLID
VAKDTQNARGVFEYPESVDLPGYKPNYDGNRRQIKEAANLLNQAKKPLIMAGHGILLGHASVELREFAEKTGIPVITTLL
GISCIPEDHPLSLGMPGMHGWVHVNRAIQECDVLFTIGARFDDRVTGKLDTFARNAKIIHVDIDPAEVGKNVITAVPIVG
DAKRVLENLTEIVNANEHSEWLNHIRDIQATRSKRQRDPESGMFTPHHVYAEFTRIMQGEFRVCTDVGQHQMWAAQLIEY
MKPHTHITSGGLGTMGFALPAAMGVQVACPNEEVWAIAGDGGFQMNLQELATVVQESLPLKICIINNGYLGMVRQWQELF
HERRYSATPMWSPDFIKLADAYGIPGVRVERAEDVAEAIQQARSTNGPFLIEFVVEREVNVYPMVAPGASISDMFEDPNE
VEIEDVVAVK

Sequences:

>Translated_570_residues
MKTLTGAQMMCEALIAEGVDVMFGYPGGAIMPFYHALPEYPQLRHILVRHEQAAGHAAEGYARTSGKVGVCVATSGPGAT
NLVTAIADAMMDSIPIVAITGQVVTSLIGKDGFQETDVTGITQPITKHNYLVTDVRDLPRVFKEAFHIARTGRPGPVLID
VAKDTQNARGVFEYPESVDLPGYKPNYDGNRRQIKEAANLLNQAKKPLIMAGHGILLGHASVELREFAEKTGIPVITTLL
GISCIPEDHPLSLGMPGMHGWVHVNRAIQECDVLFTIGARFDDRVTGKLDTFARNAKIIHVDIDPAEVGKNVITAVPIVG
DAKRVLENLTEIVNANEHSEWLNHIRDIQATRSKRQRDPESGMFTPHHVYAEFTRIMQGEFRVCTDVGQHQMWAAQLIEY
MKPHTHITSGGLGTMGFALPAAMGVQVACPNEEVWAIAGDGGFQMNLQELATVVQESLPLKICIINNGYLGMVRQWQELF
HERRYSATPMWSPDFIKLADAYGIPGVRVERAEDVAEAIQQARSTNGPFLIEFVVEREVNVYPMVAPGASISDMFEDPNE
VEIEDVVAVK
>Mature_570_residues
MKTLTGAQMMCEALIAEGVDVMFGYPGGAIMPFYHALPEYPQLRHILVRHEQAAGHAAEGYARTSGKVGVCVATSGPGAT
NLVTAIADAMMDSIPIVAITGQVVTSLIGKDGFQETDVTGITQPITKHNYLVTDVRDLPRVFKEAFHIARTGRPGPVLID
VAKDTQNARGVFEYPESVDLPGYKPNYDGNRRQIKEAANLLNQAKKPLIMAGHGILLGHASVELREFAEKTGIPVITTLL
GISCIPEDHPLSLGMPGMHGWVHVNRAIQECDVLFTIGARFDDRVTGKLDTFARNAKIIHVDIDPAEVGKNVITAVPIVG
DAKRVLENLTEIVNANEHSEWLNHIRDIQATRSKRQRDPESGMFTPHHVYAEFTRIMQGEFRVCTDVGQHQMWAAQLIEY
MKPHTHITSGGLGTMGFALPAAMGVQVACPNEEVWAIAGDGGFQMNLQELATVVQESLPLKICIINNGYLGMVRQWQELF
HERRYSATPMWSPDFIKLADAYGIPGVRVERAEDVAEAIQQARSTNGPFLIEFVVEREVNVYPMVAPGASISDMFEDPNE
VEIEDVVAVK

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=25.2688172043011, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=25.3378378378378, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=43.979057591623, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1790104, Length=557, Percent_Identity=44.8833034111311, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=547, Percent_Identity=36.9287020109689, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=28.4403669724771, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788716, Length=564, Percent_Identity=25.177304964539, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=27.336860670194, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=27.2887323943662, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=514, Percent_Identity=24.5136186770428, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=44.8453608247423, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=25.2566735112936, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=531, Percent_Identity=22.9755178907721, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=493, Percent_Identity=22.7180527383367, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922626, Length=494, Percent_Identity=27.9352226720648, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62558; Mature: 62558

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTGAQMMCEALIAEGVDVMFGYPGGAIMPFYHALPEYPQLRHILVRHEQAAGHAAEG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
YARTSGKVGVCVATSGPGATNLVTAIADAMMDSIPIVAITGQVVTSLIGKDGFQETDVTG
HHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCC
ITQPITKHNYLVTDVRDLPRVFKEAFHIARTGRPGPVLIDVAKDTQNARGVFEYPESVDL
CCCCHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCC
PGYKPNYDGNRRQIKEAANLLNQAKKPLIMAGHGILLGHASVELREFAEKTGIPVITTLL
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCHHHHHHHHHHCCCHHHHHHH
GISCIPEDHPLSLGMPGMHGWVHVNRAIQECDVLFTIGARFDDRVTGKLDTFARNAKIIH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHCCCEEEE
VDIDPAEVGKNVITAVPIVGDAKRVLENLTEIVNANEHSEWLNHIRDIQATRSKRQRDPE
EECCHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
SGMFTPHHVYAEFTRIMQGEFRVCTDVGQHQMWAAQLIEYMKPHTHITSGGLGTMGFALP
CCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
AAMGVQVACPNEEVWAIAGDGGFQMNLQELATVVQESLPLKICIINNGYLGMVRQWQELF
HHCCEEEECCCCCEEEEECCCCCEECHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
HERRYSATPMWSPDFIKLADAYGIPGVRVERAEDVAEAIQQARSTNGPFLIEFVVEREVN
HHHCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
VYPMVAPGASISDMFEDPNEVEIEDVVAVK
EEEEECCCCCHHHHHCCCCCCCCHHEEECC
>Mature Secondary Structure
MKTLTGAQMMCEALIAEGVDVMFGYPGGAIMPFYHALPEYPQLRHILVRHEQAAGHAAEG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
YARTSGKVGVCVATSGPGATNLVTAIADAMMDSIPIVAITGQVVTSLIGKDGFQETDVTG
HHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCC
ITQPITKHNYLVTDVRDLPRVFKEAFHIARTGRPGPVLIDVAKDTQNARGVFEYPESVDL
CCCCHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCC
PGYKPNYDGNRRQIKEAANLLNQAKKPLIMAGHGILLGHASVELREFAEKTGIPVITTLL
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCHHHHHHHHHHCCCHHHHHHH
GISCIPEDHPLSLGMPGMHGWVHVNRAIQECDVLFTIGARFDDRVTGKLDTFARNAKIIH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHCCCEEEE
VDIDPAEVGKNVITAVPIVGDAKRVLENLTEIVNANEHSEWLNHIRDIQATRSKRQRDPE
EECCHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
SGMFTPHHVYAEFTRIMQGEFRVCTDVGQHQMWAAQLIEYMKPHTHITSGGLGTMGFALP
CCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
AAMGVQVACPNEEVWAIAGDGGFQMNLQELATVVQESLPLKICIINNGYLGMVRQWQELF
HHCCEEEECCCCCEEEEECCCCCEECHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
HERRYSATPMWSPDFIKLADAYGIPGVRVERAEDVAEAIQQARSTNGPFLIEFVVEREVN
HHHCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
VYPMVAPGASISDMFEDPNEVEIEDVVAVK
EEEEECCCCCHHHHHCCCCCCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]