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The map label for this gene is cobS

Identifier: 159900903

GI number: 159900903

Start: 5608663

End: 5609412

Strand: Direct

Name: cobS

Synonym: Haur_4390

Alternate gene names: 159900903

Gene position: 5608663-5609412 (Clockwise)

Preceding gene: 159900902

Following gene: 159900905

Centisome position: 88.37

GC content: 53.87

Gene sequence:

>750_bases
GTGATCAAGCATCTGGCTGAGGCGTTGCGTTTTCTCACAATCTTGCCAATTCCAGGCAAACCTGCGCTCAGCGAACAAGC
CTTAGTCCGTTCGATGGTGGCTTTTCCGCTAGCTGGAACGCTGATTGGCGGTTTGGTTGCGGCGACATGGTTTGGGGCAA
CCTGGCTGTGGGGCACGACTACCGGCAGTTTATGTGCAATTCTCACCTGGGGGGCAATTACCAGCGGCTTGCATTTGGAT
GGAATTGCAGATAGTGCCGATGCGCTGTTTAGCTGGCGTTCGCGTGAGCGCAAGCTCGAAATTATGAAAGATAGCCGAAT
TGGCACGATGGGCGCAATCGCATTAATCAGTATTTTGCTGCTGAAATGGCTGTTTGTGCTTGGTTGCGGCGATCTTGCTT
GGCGAGCATTGATTGTGGCTCCAACGCTTGGCCGTTGGGTCGATATTATCGGAATTTTCTGGTTTCCGCCTGCGGCTGAA
GGTGGGCTTGGGCGCACATTTCACGACCATACGCGGCGCAGCGATTTTTGGTGGGCGACGAGCTGTGCTGGTTTGGTTGC
GGCGGGGTTGCTCTGGTGGTGGGCTGGGTTAATTTTTGCCGTCGTGATTGTGGCTACGATCATTATTGCCCGCTGGATGG
TGCGCTCGTTGGGTGGCTTAACTGGTGATACCTATGGGGCGCTCTGTGAAATTGCCGAAATGCTGGTGTTGGCGGTGGTT
GCGGCATTGGTTAATCATCAAGTGCTTTAA

Upstream 100 bases:

>100_bases
CCTGTGGCGGTTTCCGAAGAAGTAGCCTAAGTTTTTTGCTTGACGCAAGGTTTTATCGTATTTCGATAAAACCTTGCGTT
GCTGTTTAAAGGAGCTGATG

Downstream 100 bases:

>100_bases
AATACCACCGCGCCATCAAAGCTTGGCGCGGGTAAGTACGAGAATTCATTGCTGAACTCGCTTCTGCTCGGATATATGTA
CGGCAGTTGTTAGCAAATGT

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIKHLAEALRFLTILPIPGKPALSEQALVRSMVAFPLAGTLIGGLVAATWFGATWLWGTTTGSLCAILTWGAITSGLHLD
GIADSADALFSWRSRERKLEIMKDSRIGTMGAIALISILLLKWLFVLGCGDLAWRALIVAPTLGRWVDIIGIFWFPPAAE
GGLGRTFHDHTRRSDFWWATSCAGLVAAGLLWWWAGLIFAVVIVATIIIARWMVRSLGGLTGDTYGALCEIAEMLVLAVV
AALVNHQVL

Sequences:

>Translated_249_residues
MIKHLAEALRFLTILPIPGKPALSEQALVRSMVAFPLAGTLIGGLVAATWFGATWLWGTTTGSLCAILTWGAITSGLHLD
GIADSADALFSWRSRERKLEIMKDSRIGTMGAIALISILLLKWLFVLGCGDLAWRALIVAPTLGRWVDIIGIFWFPPAAE
GGLGRTFHDHTRRSDFWWATSCAGLVAAGLLWWWAGLIFAVVIVATIIIARWMVRSLGGLTGDTYGALCEIAEMLVLAVV
AALVNHQVL
>Mature_249_residues
MIKHLAEALRFLTILPIPGKPALSEQALVRSMVAFPLAGTLIGGLVAATWFGATWLWGTTTGSLCAILTWGAITSGLHLD
GIADSADALFSWRSRERKLEIMKDSRIGTMGAIALISILLLKWLFVLGCGDLAWRALIVAPTLGRWVDIIGIFWFPPAAE
GGLGRTFHDHTRRSDFWWATSCAGLVAAGLLWWWAGLIFAVVIVATIIIARWMVRSLGGLTGDTYGALCEIAEMLVLAVV
AALVNHQVL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=238, Percent_Identity=35.2941176470588, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_HERA2 (A9AYY2)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547150.1
- GeneID:   5736240
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4390
- HOGENOM:   HBG729392
- OMA:   IMKDPTS
- ProtClustDB:   CLSK975423
- BioCyc:   HAUR316274:HAUR_4390-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26919; Mature: 26919

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a5cc70c)-; HASH(0x16cebbd8)-; HASH(0x18ac578c)-; HASH(0x17486dcc)-; HASH(0x18efca28)-; HASH(0x19026b78)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCH
>Mature Secondary Structure
MIKHLAEALRFLTILPIPGKPALSEQALVRSMVAFPLAGTLIGGLVAATWFGATWLWGTT
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGSLCAILTWGAITSGLHLDGIADSADALFSWRSRERKLEIMKDSRIGTMGAIALISILL
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKWLFVLGCGDLAWRALIVAPTLGRWVDIIGIFWFPPAAEGGLGRTFHDHTRRSDFWWAT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHH
SCAGLVAAGLLWWWAGLIFAVVIVATIIIARWMVRSLGGLTGDTYGALCEIAEMLVLAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AALVNHQVL
HHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA