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The map label for this gene is nadE [H]

Identifier: 159900802

GI number: 159900802

Start: 5476218

End: 5478086

Strand: Reverse

Name: nadE [H]

Synonym: Haur_4289

Alternate gene names: 159900802

Gene position: 5478086-5476218 (Counterclockwise)

Preceding gene: 159900803

Following gene: 159900801

Centisome position: 86.32

GC content: 53.18

Gene sequence:

>1869_bases
ATGTACACTTTAGCTTTAGCCCAATTTCGCCCGCGCAAAGGGCATTATGCCGCCAATTTGGCGCGGCTTGGCGAAATTTT
CGCCCAACTTGGCACAACCGAACGTCAGCCCGATGTGCTGATGTTGCCTGAAACCGCCTTAACTGGCTATTTCCTTGAGG
GCGGCGTGCGTGAGCAGGCAGTTACCGCAGGTCAATTGTTTCACGATTTGCAACAAACCTATGTGGCGGCACGCGGCGCT
GATGCACCAGCCCTCGATATTGTGATTGGCTTTTACGAACGTTGGCGCGAACGTTTTTACAATAGCGCTCTGTATGCCAC
GATTGGCAGCGATCAGAGCTTGGCCGGCATTCGCCATGTGCATCGTAAGATGTTTTTGCCAACCTACGGCGTGTTTGATG
AAGCGCGTTTTGTTGAGGCTGGCCGCCAAATTGCGGCCTTCGATACCCGTTTTGGGCGGGTGGCAATCTTGATTTGCGAG
GATGCATGGCACTCGCTGAGCGGTACGGTCGCGGCGCTTGATGGGGCGCAGATGCTCTACGTCGTCAGTGCTTCGCCTGC
CCGTGGAGCCAACGAAGATCGTCCGAGCAATCTCGCCCGTTGGGATGACCGGATTCGTGAAATTGCTGGCGAACATGGGG
TCTATCTGGCGGTTTGCCAAATTGTCGGCTTTGAAGGTGGCAAGGGTTTTGCTGGTGGTTCAGTCGTGGTTGGTCCCCGC
TCCGATGAACGCGCTCGCGCTCCGTTGTGGTCGGAAGCAGTAATCTTGGTCGATATTGATCTCAGCGATTTGACTTTGGC
CCGTGCTGATTTGCCATTATTGGCTGATTTGGAAGAACGCTTGCCCTTTATGTTGCGCGATGTGCATGCTGCCAGCGAGG
GCCAACATCGCCCAAGCACGTGGGATACGGCGCTTTCCAGCGATTCGCAAGGCCTCAACGATGCCAGTTCGTATCAACGT
ACCGTCGATAGCGAAGGCGAAAAACGCGCCAAAGCCACAGTTGGCAATCAAAGCTTTCCAATTATTGCTGCACCACGTTT
TGATCCCGAGAGCGACGAGCCACTGCGGATCAATCCTGAATTGACGATTAATTGGTTGGTCGAGTTTTTGCGCGATGAAG
TTGGCTTTCGGCGTGGCTTTAAACAAGTTGTGATTGGGCTTTCGGGCGGGGTTGATTCGGCCTTAACCACCTATTTGTGT
GCTAAGGCTTTTGGCGCTGAAAATGTGCTCGCCGTGCGCATGCCCTATCGCACTTCATCGTCGGATAGTTTAGACCACGC
CCAGTTGGTGATCGACGATCTAGGCATTCAGCATCGCACGATCGAAATTACTAATGCGGTTGAAGGCTATTTGAGCTTTG
AGCCAGATGCTGATGGCCGACGGCGGGGCAATGTGATGGCTCGCACCCGGATGATTGTGTTGTTTGATCAATCGCAAAAG
CTCGGGTGTATTCCGATTGGCACTGGCAACAAAACCGAGCGTTTATTTGGCTATTACACGTGGCATGCCGATGATGCTCC
GCCAGTCAATCCTTTGGGCGACTTGTTCAAGACCCAAGTTTGGGAATTGGCTTCGGCGATTGGCGTACCTGATGTGATTG
TGCATAAGCCAGCTTCGGCTGATTTGGTGGTTGGTCAAACCGATGAAGATGATTTTGGCATCTCGTATCGCAAGGCTGAT
CGAGTGCTGGCCTATTTGTTGAGCGGTTATCGACCTGAGCAATTGGTTGCGCGTGGCTTCAATCCCGATGAAGTAGCGAT
TGTTCAGCGGCGGGTCAATTCGACTCACTGGAAACGCCATTTGCCCAGCACTGCCATGCTCTCAAGCACCGCCATCGGCA
AATACTACTTGCGGCCAGTCGATTATTAA

Upstream 100 bases:

>100_bases
GACGAACAATAAGGCAAACGTCAGAAATCAAAAGTCAAAATACTCGGAATAAGCTATTTTGCCTTTTGCCCTTTGCCTTC
TAACTTTACTGGGGGTGTTT

Downstream 100 bases:

>100_bases
AGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAAGTCAGAATCGGTGGTG
TTTTTTGCCTTTTGATTTCT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MYTLALAQFRPRKGHYAANLARLGEIFAQLGTTERQPDVLMLPETALTGYFLEGGVREQAVTAGQLFHDLQQTYVAARGA
DAPALDIVIGFYERWRERFYNSALYATIGSDQSLAGIRHVHRKMFLPTYGVFDEARFVEAGRQIAAFDTRFGRVAILICE
DAWHSLSGTVAALDGAQMLYVVSASPARGANEDRPSNLARWDDRIREIAGEHGVYLAVCQIVGFEGGKGFAGGSVVVGPR
SDERARAPLWSEAVILVDIDLSDLTLARADLPLLADLEERLPFMLRDVHAASEGQHRPSTWDTALSSDSQGLNDASSYQR
TVDSEGEKRAKATVGNQSFPIIAAPRFDPESDEPLRINPELTINWLVEFLRDEVGFRRGFKQVVIGLSGGVDSALTTYLC
AKAFGAENVLAVRMPYRTSSSDSLDHAQLVIDDLGIQHRTIEITNAVEGYLSFEPDADGRRRGNVMARTRMIVLFDQSQK
LGCIPIGTGNKTERLFGYYTWHADDAPPVNPLGDLFKTQVWELASAIGVPDVIVHKPASADLVVGQTDEDDFGISYRKAD
RVLAYLLSGYRPEQLVARGFNPDEVAIVQRRVNSTHWKRHLPSTAMLSSTAIGKYYLRPVDY

Sequences:

>Translated_622_residues
MYTLALAQFRPRKGHYAANLARLGEIFAQLGTTERQPDVLMLPETALTGYFLEGGVREQAVTAGQLFHDLQQTYVAARGA
DAPALDIVIGFYERWRERFYNSALYATIGSDQSLAGIRHVHRKMFLPTYGVFDEARFVEAGRQIAAFDTRFGRVAILICE
DAWHSLSGTVAALDGAQMLYVVSASPARGANEDRPSNLARWDDRIREIAGEHGVYLAVCQIVGFEGGKGFAGGSVVVGPR
SDERARAPLWSEAVILVDIDLSDLTLARADLPLLADLEERLPFMLRDVHAASEGQHRPSTWDTALSSDSQGLNDASSYQR
TVDSEGEKRAKATVGNQSFPIIAAPRFDPESDEPLRINPELTINWLVEFLRDEVGFRRGFKQVVIGLSGGVDSALTTYLC
AKAFGAENVLAVRMPYRTSSSDSLDHAQLVIDDLGIQHRTIEITNAVEGYLSFEPDADGRRRGNVMARTRMIVLFDQSQK
LGCIPIGTGNKTERLFGYYTWHADDAPPVNPLGDLFKTQVWELASAIGVPDVIVHKPASADLVVGQTDEDDFGISYRKAD
RVLAYLLSGYRPEQLVARGFNPDEVAIVQRRVNSTHWKRHLPSTAMLSSTAIGKYYLRPVDY
>Mature_622_residues
MYTLALAQFRPRKGHYAANLARLGEIFAQLGTTERQPDVLMLPETALTGYFLEGGVREQAVTAGQLFHDLQQTYVAARGA
DAPALDIVIGFYERWRERFYNSALYATIGSDQSLAGIRHVHRKMFLPTYGVFDEARFVEAGRQIAAFDTRFGRVAILICE
DAWHSLSGTVAALDGAQMLYVVSASPARGANEDRPSNLARWDDRIREIAGEHGVYLAVCQIVGFEGGKGFAGGSVVVGPR
SDERARAPLWSEAVILVDIDLSDLTLARADLPLLADLEERLPFMLRDVHAASEGQHRPSTWDTALSSDSQGLNDASSYQR
TVDSEGEKRAKATVGNQSFPIIAAPRFDPESDEPLRINPELTINWLVEFLRDEVGFRRGFKQVVIGLSGGVDSALTTYLC
AKAFGAENVLAVRMPYRTSSSDSLDHAQLVIDDLGIQHRTIEITNAVEGYLSFEPDADGRRRGNVMARTRMIVLFDQSQK
LGCIPIGTGNKTERLFGYYTWHADDAPPVNPLGDLFKTQVWELASAIGVPDVIVHKPASADLVVGQTDEDDFGISYRKAD
RVLAYLLSGYRPEQLVARGFNPDEVAIVQRRVNSTHWKRHLPSTAMLSSTAIGKYYLRPVDY

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=241, Percent_Identity=29.4605809128631, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 68715; Mature: 68715

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLALAQFRPRKGHYAANLARLGEIFAQLGTTERQPDVLMLPETALTGYFLEGGVREQA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCHHHH
VTAGQLFHDLQQTYVAARGADAPALDIVIGFYERWRERFYNSALYATIGSDQSLAGIRHV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
HRKMFLPTYGVFDEARFVEAGRQIAAFDTRFGRVAILICEDAWHSLSGTVAALDGAQMLY
HHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEECCCEEEE
VVSASPARGANEDRPSNLARWDDRIREIAGEHGVYLAVCQIVGFEGGKGFAGGSVVVGPR
EEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCCCCCEEEECCC
SDERARAPLWSEAVILVDIDLSDLTLARADLPLLADLEERLPFMLRDVHAASEGQHRPST
CCCCCCCCCCCCEEEEEEECCCCCEEHHCCCCHHHCHHHHCCHHHHHHHHHCCCCCCCCC
WDTALSSDSQGLNDASSYQRTVDSEGEKRAKATVGNQSFPIIAAPRFDPESDEPLRINPE
HHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCEEECCC
LTINWLVEFLRDEVGFRRGFKQVVIGLSGGVDSALTTYLCAKAFGAENVLAVRMPYRTSS
CCHHHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
SDSLDHAQLVIDDLGIQHRTIEITNAVEGYLSFEPDADGRRRGNVMARTRMIVLFDQSQK
CCCCHHHHHHHHHCCCCEEEEEEECHHCCEEECCCCCCCCCCCCEEEEEEEEEEEECCCC
LGCIPIGTGNKTERLFGYYTWHADDAPPVNPLGDLFKTQVWELASAIGVPDVIVHKPASA
CEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DLVVGQTDEDDFGISYRKADRVLAYLLSGYRPEQLVARGFNPDEVAIVQRRVNSTHWKRH
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LPSTAMLSSTAIGKYYLRPVDY
CCCHHHHHHHCCCCEEECCCCC
>Mature Secondary Structure
MYTLALAQFRPRKGHYAANLARLGEIFAQLGTTERQPDVLMLPETALTGYFLEGGVREQA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCHHHH
VTAGQLFHDLQQTYVAARGADAPALDIVIGFYERWRERFYNSALYATIGSDQSLAGIRHV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
HRKMFLPTYGVFDEARFVEAGRQIAAFDTRFGRVAILICEDAWHSLSGTVAALDGAQMLY
HHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEECCCEEEE
VVSASPARGANEDRPSNLARWDDRIREIAGEHGVYLAVCQIVGFEGGKGFAGGSVVVGPR
EEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCCCCCEEEECCC
SDERARAPLWSEAVILVDIDLSDLTLARADLPLLADLEERLPFMLRDVHAASEGQHRPST
CCCCCCCCCCCCEEEEEEECCCCCEEHHCCCCHHHCHHHHCCHHHHHHHHHCCCCCCCCC
WDTALSSDSQGLNDASSYQRTVDSEGEKRAKATVGNQSFPIIAAPRFDPESDEPLRINPE
HHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCEEECCC
LTINWLVEFLRDEVGFRRGFKQVVIGLSGGVDSALTTYLCAKAFGAENVLAVRMPYRTSS
CCHHHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
SDSLDHAQLVIDDLGIQHRTIEITNAVEGYLSFEPDADGRRRGNVMARTRMIVLFDQSQK
CCCCHHHHHHHHHCCCCEEEEEEECHHCCEEECCCCCCCCCCCCEEEEEEEEEEEECCCC
LGCIPIGTGNKTERLFGYYTWHADDAPPVNPLGDLFKTQVWELASAIGVPDVIVHKPASA
CEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DLVVGQTDEDDFGISYRKADRVLAYLLSGYRPEQLVARGFNPDEVAIVQRRVNSTHWKRH
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LPSTAMLSSTAIGKYYLRPVDY
CCCHHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]