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The map label for this gene is amtB [C]

Identifier: 159900760

GI number: 159900760

Start: 5418649

End: 5420136

Strand: Reverse

Name: amtB [C]

Synonym: Haur_4247

Alternate gene names: 159900760

Gene position: 5420136-5418649 (Counterclockwise)

Preceding gene: 159900761

Following gene: 159900759

Centisome position: 85.4

GC content: 52.69

Gene sequence:

>1488_bases
GTGGAAGAGCAACTCAACTCATTGACGGTCAACGCAAACATCTTGTGGGTCTTGCTCGCTGCTTTTCTGGTCTTCTTTAT
GCAAGCTGGGTTTGCCTTGGTTGAAGCAGGTTTCACTAGGGCAAAAAATGTCGCGCACACCATGCTCATGAACTTGATGG
TGTTTTGTATTGGGGCGATTGGCTTCTGGATTTGTGGCTTTGCCTTTGCCTTCGGTGGAGTCAACCATACCTACCCAGCC
ACAGGCGCAAACGCTGAATGGAACTTCGGCCCAGTTACCTTGGGCGCTTGGGGCGATGCGCTCTCCTCAAATCTCTCCTT
CGGTGATCAATGGGGCGTGCTTGGCACCAGCGGCTTCTTCCTGAATGGCATTGGCATGAGTGCTGCTGGGATTTTTGCCT
TCTTTATGTTCCAAATGGTCTTTATGGATACCGCCGCCACGATTCCAACGGGCAGCGGGGCTGAACGCATCAAGTTCATT
GGTTTCGTGATTATGGGCTTTTTGGTCAGTATGTTCGTCTATCCACTTTCGGCCAACTGGGTTTGGGGCGGTGGCTGGAT
GGCCAATATGGGCCGCACGCTTGGGCTTGGCAACGGCGCAGTCGATTTTGCTGGCTCAGGGGTTGTGCACATGACTGGTG
GCGCAGTTGGTTTGGCAGTTGCGCTGGTGCTTGGGCCACGGATCGGCAAATTTAATAAAGATGGCAGTGCCAACACGATT
CTTGGGCACAACTTACCAATGGGTGTTTTGGGCGCAATCATCCTCTTCTTTGGCTGGTTCGGTTTCAATCCAGGTAGCTC
ATTGGGTATCCAAGGTTCGTTTATGAACCTAACCGCCTTGGCCGCCTTGAATACCTTGATCGCTGGAGCTGCTGGTGGCA
TCAGCGCCATGACCTACACATGGCTGAAAAATAAAAAGCCCGATCCAGGCATGAGTGTCAATGGTTTATTAGCAGGCTTG
GTGGCGGTAACCGCGCCTTGTGCCTTTATCACGCCGGTTGCCGCAGCAATTATTGGGGCAGTTGGCGGGGTTTTGGTGAT
TTTCGCCAGCATTCTGTTGGAAAAACTCAAAATCGACGATCCAGTTGGGGCAGTGCCTGTGCACTTGTTCAATGGCTTTT
GGGGTGTGATTGCGGTTGGTCTCTTTGCCAACGGCAACCCCGATACCGCTAGCTGGAACGGCATCGATGGTCAAGCGGTA
ACTGGCTTGTTCTATGGTGGTGGGTTCAGCCAACTCTTTGCTCAATTGATCGAAGGCTTGGGCATTGGCATCACGGCATT
TGTTTTATCGTTTGTGGTCTTCAAAGCCTTGGCCAAACTTGGCCTAATGCGTTCACGCGCTGAAGACGAAGTAGCAGGCT
TGGATTTACCAGAAATGGGCATGCCTGGCTATGTTTCCGATGGCGCATATATGCCCGAAACTGGGGCAGCCCCTGCTCCT
AGCGAAGAACCAGCTACGGCTGGCTTGCCCGCTGGCGCTGCTGCTTAA

Upstream 100 bases:

>100_bases
AATCCCCTCTTGCATTCCAGCCGTCAGTGTTGACGTGAGCCGCGTTGCAGCGACATGCATTTGACCCTATTCCGATACGG
GCTACAGAAAGGAAACGCCT

Downstream 100 bases:

>100_bases
CTCAATTCAAACCTCAAACGCCGTAGGTTCGAGCCTACGGCGTTTTTCATTTTAGCCGCCAGCCACATAGATATGGACTA
TCGTTGCAATTGAAAGCTCT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MEEQLNSLTVNANILWVLLAAFLVFFMQAGFALVEAGFTRAKNVAHTMLMNLMVFCIGAIGFWICGFAFAFGGVNHTYPA
TGANAEWNFGPVTLGAWGDALSSNLSFGDQWGVLGTSGFFLNGIGMSAAGIFAFFMFQMVFMDTAATIPTGSGAERIKFI
GFVIMGFLVSMFVYPLSANWVWGGGWMANMGRTLGLGNGAVDFAGSGVVHMTGGAVGLAVALVLGPRIGKFNKDGSANTI
LGHNLPMGVLGAIILFFGWFGFNPGSSLGIQGSFMNLTALAALNTLIAGAAGGISAMTYTWLKNKKPDPGMSVNGLLAGL
VAVTAPCAFITPVAAAIIGAVGGVLVIFASILLEKLKIDDPVGAVPVHLFNGFWGVIAVGLFANGNPDTASWNGIDGQAV
TGLFYGGGFSQLFAQLIEGLGIGITAFVLSFVVFKALAKLGLMRSRAEDEVAGLDLPEMGMPGYVSDGAYMPETGAAPAP
SEEPATAGLPAGAAA

Sequences:

>Translated_495_residues
MEEQLNSLTVNANILWVLLAAFLVFFMQAGFALVEAGFTRAKNVAHTMLMNLMVFCIGAIGFWICGFAFAFGGVNHTYPA
TGANAEWNFGPVTLGAWGDALSSNLSFGDQWGVLGTSGFFLNGIGMSAAGIFAFFMFQMVFMDTAATIPTGSGAERIKFI
GFVIMGFLVSMFVYPLSANWVWGGGWMANMGRTLGLGNGAVDFAGSGVVHMTGGAVGLAVALVLGPRIGKFNKDGSANTI
LGHNLPMGVLGAIILFFGWFGFNPGSSLGIQGSFMNLTALAALNTLIAGAAGGISAMTYTWLKNKKPDPGMSVNGLLAGL
VAVTAPCAFITPVAAAIIGAVGGVLVIFASILLEKLKIDDPVGAVPVHLFNGFWGVIAVGLFANGNPDTASWNGIDGQAV
TGLFYGGGFSQLFAQLIEGLGIGITAFVLSFVVFKALAKLGLMRSRAEDEVAGLDLPEMGMPGYVSDGAYMPETGAAPAP
SEEPATAGLPAGAAA
>Mature_495_residues
MEEQLNSLTVNANILWVLLAAFLVFFMQAGFALVEAGFTRAKNVAHTMLMNLMVFCIGAIGFWICGFAFAFGGVNHTYPA
TGANAEWNFGPVTLGAWGDALSSNLSFGDQWGVLGTSGFFLNGIGMSAAGIFAFFMFQMVFMDTAATIPTGSGAERIKFI
GFVIMGFLVSMFVYPLSANWVWGGGWMANMGRTLGLGNGAVDFAGSGVVHMTGGAVGLAVALVLGPRIGKFNKDGSANTI
LGHNLPMGVLGAIILFFGWFGFNPGSSLGIQGSFMNLTALAALNTLIAGAAGGISAMTYTWLKNKKPDPGMSVNGLLAGL
VAVTAPCAFITPVAAAIIGAVGGVLVIFASILLEKLKIDDPVGAVPVHLFNGFWGVIAVGLFANGNPDTASWNGIDGQAV
TGLFYGGGFSQLFAQLIEGLGIGITAFVLSFVVFKALAKLGLMRSRAEDEVAGLDLPEMGMPGYVSDGAYMPETGAAPAP
SEEPATAGLPAGAAA

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=462, Percent_Identity=29.8701298701299, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=455, Percent_Identity=33.6263736263736, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17550180, Length=459, Percent_Identity=34.4226579520697, Blast_Score=221, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17550182, Length=469, Percent_Identity=33.4754797441365, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI133931100, Length=470, Percent_Identity=30, Blast_Score=203, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324187, Length=494, Percent_Identity=26.5182186234818, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=461, Percent_Identity=26.6811279826464, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=458, Percent_Identity=27.292576419214, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078314, Length=453, Percent_Identity=33.112582781457, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50991; Mature: 50991

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQLNSLTVNANILWVLLAAFLVFFMQAGFALVEAGFTRAKNVAHTMLMNLMVFCIGAI
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFWICGFAFAFGGVNHTYPATGANAEWNFGPVTLGAWGDALSSNLSFGDQWGVLGTSGFF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCE
LNGIGMSAAGIFAFFMFQMVFMDTAATIPTGSGAERIKFIGFVIMGFLVSMFVYPLSANW
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VWGGGWMANMGRTLGLGNGAVDFAGSGVVHMTGGAVGLAVALVLGPRIGKFNKDGSANTI
EECCCHHHHCCCEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LGHNLPMGVLGAIILFFGWFGFNPGSSLGIQGSFMNLTALAALNTLIAGAAGGISAMTYT
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WLKNKKPDPGMSVNGLLAGLVAVTAPCAFITPVAAAIIGAVGGVLVIFASILLEKLKIDD
HHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVGAVPVHLFNGFWGVIAVGLFANGNPDTASWNGIDGQAVTGLFYGGGFSQLFAQLIEGL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCC
GIGITAFVLSFVVFKALAKLGLMRSRAEDEVAGLDLPEMGMPGYVSDGAYMPETGAAPAP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SEEPATAGLPAGAAA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MEEQLNSLTVNANILWVLLAAFLVFFMQAGFALVEAGFTRAKNVAHTMLMNLMVFCIGAI
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFWICGFAFAFGGVNHTYPATGANAEWNFGPVTLGAWGDALSSNLSFGDQWGVLGTSGFF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCE
LNGIGMSAAGIFAFFMFQMVFMDTAATIPTGSGAERIKFIGFVIMGFLVSMFVYPLSANW
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VWGGGWMANMGRTLGLGNGAVDFAGSGVVHMTGGAVGLAVALVLGPRIGKFNKDGSANTI
EECCCHHHHCCCEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LGHNLPMGVLGAIILFFGWFGFNPGSSLGIQGSFMNLTALAALNTLIAGAAGGISAMTYT
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WLKNKKPDPGMSVNGLLAGLVAVTAPCAFITPVAAAIIGAVGGVLVIFASILLEKLKIDD
HHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVGAVPVHLFNGFWGVIAVGLFANGNPDTASWNGIDGQAVTGLFYGGGFSQLFAQLIEGL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCC
GIGITAFVLSFVVFKALAKLGLMRSRAEDEVAGLDLPEMGMPGYVSDGAYMPETGAAPAP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SEEPATAGLPAGAAA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]