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The map label for this gene is cls [H]

Identifier: 159900742

GI number: 159900742

Start: 5390876

End: 5392129

Strand: Direct

Name: cls [H]

Synonym: Haur_4229

Alternate gene names: 159900742

Gene position: 5390876-5392129 (Clockwise)

Preceding gene: 159900741

Following gene: 159900745

Centisome position: 84.94

GC content: 46.25

Gene sequence:

>1254_bases
ATGCGTTGGCTCAAAGCTGGGTTATGGTTTCTGACTGAGTGGGTGCTTTCGCTGCCACGTTTGCTGCTGGCCAGCATTCT
GGCTCAATTGGGCTTGATGGGTGCGCTGATGGCTTACGCTAAGTTGCGCAATCGCAGCGTCGAAAATAGCTTTCCCCAGC
TTGATTTGCCGCCAATCGATTTGGGCGAGCATAGCGTGCAATTATTTGATGATGGTGGCTCAGTCTTTCGCCAAATGCTG
TTTGATATTTCCCTAGCCAAAGAATCGATTTTGCTCGAAAGCTATATTTTCGAGCGTGATGAGGTTGGGCTAGCCTTCAA
ACGGGCATTAATTCGCAAAGCCCGCGAAGGTGTGAAAATTTATGTAACCTTCGATGGCATTGGCACGTTGCATGTGCCCG
AACGCTTTAAGTTATTTGGCAAGCGCCGCAATATTCGGGTCTTTGAATTTGGCCGCCTCAAATCAATTCGCTCATTTTTC
GATACGAACATGTGGATTCGCACCCATCGCAAAATTTTGGTGATCGATGGCAAAATTGGCTATTTAGGCGGCATGAATAT
TGGCCGCAACTATGCCCGAACTTGGCGTGATACTCATTTAAAAATTGAAGGCCCAATCGCAGCCAATATTGCCGAAGAAT
TTATCGCTCTGTGGAATAAATACAATAAAAAACATAAAATCAATTTAAGTTACGCAACTACTAGCGATCAACAAGTGGGA
ATTTGTGCCAACGACCCAATTGAATATCAACTGCCGATTCGCCAAACCTATCTTGATGCAATTAATGATGCCAAACACTA
TATTTACATTTCAAATGCCTATTTCTTGCCCGACCCGCTGATTCGAGCAACGCTGATTGATGCAGCCAAACGCGGGGTCG
ATATTCAAATTATGGTGCCCGAAATCTCCGATAATATCGTAGTCGATTGGATTTGTCGCGGTTTATTGGGAGAATTAATG
GAGCATGGCGCACGGGTGCTGCTCTATCGTGGCACCATGATTCACGCCAAAACCATGACCGTTGATGGGTTGTGGTCAAC
CGTGGGTAGCGCTAATTTAGATACCCGCAGTTTGGCCGCGAATTACGAAATCAACGCCACGATCAAGCATCCAGCTTTTG
CCCGCCAAATGGAAGCCATGTTTTTGAATGATCGAGCGCAATCGCGTGAAATTAATTATCAAACCTGGCGTAGTCGTTCG
ATTTTTATTCACCTCGGCGAGCAAGTGCTGCAACCAGCGCGAGGCCTGTTTTAG

Upstream 100 bases:

>100_bases
AGTGTTGTTTAAGCGTGGCCGTGCTATAGTGGTGGCATTGGAATTGCGACAAGCAAGCTTGGCCTCGGCAATTTGAGGCT
AAAGCTGCGAAAGGATCGCT

Downstream 100 bases:

>100_bases
CAAGGCAAACATCAAAAATCAAAGGGCAAAAAAGCAGAAGCCAAAAAGCGAAGCAATACTACTTTTGCTTTTTGACTTCT
GCCTTTTGATTTTCTTAGCG

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRWLKAGLWFLTEWVLSLPRLLLASILAQLGLMGALMAYAKLRNRSVENSFPQLDLPPIDLGEHSVQLFDDGGSVFRQML
FDISLAKESILLESYIFERDEVGLAFKRALIRKAREGVKIYVTFDGIGTLHVPERFKLFGKRRNIRVFEFGRLKSIRSFF
DTNMWIRTHRKILVIDGKIGYLGGMNIGRNYARTWRDTHLKIEGPIAANIAEEFIALWNKYNKKHKINLSYATTSDQQVG
ICANDPIEYQLPIRQTYLDAINDAKHYIYISNAYFLPDPLIRATLIDAAKRGVDIQIMVPEISDNIVVDWICRGLLGELM
EHGARVLLYRGTMIHAKTMTVDGLWSTVGSANLDTRSLAANYEINATIKHPAFARQMEAMFLNDRAQSREINYQTWRSRS
IFIHLGEQVLQPARGLF

Sequences:

>Translated_417_residues
MRWLKAGLWFLTEWVLSLPRLLLASILAQLGLMGALMAYAKLRNRSVENSFPQLDLPPIDLGEHSVQLFDDGGSVFRQML
FDISLAKESILLESYIFERDEVGLAFKRALIRKAREGVKIYVTFDGIGTLHVPERFKLFGKRRNIRVFEFGRLKSIRSFF
DTNMWIRTHRKILVIDGKIGYLGGMNIGRNYARTWRDTHLKIEGPIAANIAEEFIALWNKYNKKHKINLSYATTSDQQVG
ICANDPIEYQLPIRQTYLDAINDAKHYIYISNAYFLPDPLIRATLIDAAKRGVDIQIMVPEISDNIVVDWICRGLLGELM
EHGARVLLYRGTMIHAKTMTVDGLWSTVGSANLDTRSLAANYEINATIKHPAFARQMEAMFLNDRAQSREINYQTWRSRS
IFIHLGEQVLQPARGLF
>Mature_417_residues
MRWLKAGLWFLTEWVLSLPRLLLASILAQLGLMGALMAYAKLRNRSVENSFPQLDLPPIDLGEHSVQLFDDGGSVFRQML
FDISLAKESILLESYIFERDEVGLAFKRALIRKAREGVKIYVTFDGIGTLHVPERFKLFGKRRNIRVFEFGRLKSIRSFF
DTNMWIRTHRKILVIDGKIGYLGGMNIGRNYARTWRDTHLKIEGPIAANIAEEFIALWNKYNKKHKINLSYATTSDQQVG
ICANDPIEYQLPIRQTYLDAINDAKHYIYISNAYFLPDPLIRATLIDAAKRGVDIQIMVPEISDNIVVDWICRGLLGELM
EHGARVLLYRGTMIHAKTMTVDGLWSTVGSANLDTRSLAANYEINATIKHPAFARQMEAMFLNDRAQSREINYQTWRSRS
IFIHLGEQVLQPARGLF

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=366, Percent_Identity=27.0491803278689, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787007, Length=328, Percent_Identity=29.8780487804878, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47814; Mature: 47814

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWLKAGLWFLTEWVLSLPRLLLASILAQLGLMGALMAYAKLRNRSVENSFPQLDLPPID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LGEHSVQLFDDGGSVFRQMLFDISLAKESILLESYIFERDEVGLAFKRALIRKAREGVKI
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEE
YVTFDGIGTLHVPERFKLFGKRRNIRVFEFGRLKSIRSFFDTNMWIRTHRKILVIDGKIG
EEEECCCEEEECCHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCEEEECCEEEEEECCEE
YLGGMNIGRNYARTWRDTHLKIEGPIAANIAEEFIALWNKYNKKHKINLSYATTSDQQVG
EECCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEE
ICANDPIEYQLPIRQTYLDAINDAKHYIYISNAYFLPDPLIRATLIDAAKRGVDIQIMVP
EECCCCCEEECCHHHHHHHHHCCCCEEEEEECEEECCCHHHHHHHHHHHHCCCEEEEEEC
EISDNIVVDWICRGLLGELMEHGARVLLYRGTMIHAKTMTVDGLWSTVGSANLDTRSLAA
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEEEEEEEECHHHHHCCCCCCCHHHEEE
NYEINATIKHPAFARQMEAMFLNDRAQSREINYQTWRSRSIFIHLGEQVLQPARGLF
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECHHHHHHHCCCC
>Mature Secondary Structure
MRWLKAGLWFLTEWVLSLPRLLLASILAQLGLMGALMAYAKLRNRSVENSFPQLDLPPID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LGEHSVQLFDDGGSVFRQMLFDISLAKESILLESYIFERDEVGLAFKRALIRKAREGVKI
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEE
YVTFDGIGTLHVPERFKLFGKRRNIRVFEFGRLKSIRSFFDTNMWIRTHRKILVIDGKIG
EEEECCCEEEECCHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCEEEECCEEEEEECCEE
YLGGMNIGRNYARTWRDTHLKIEGPIAANIAEEFIALWNKYNKKHKINLSYATTSDQQVG
EECCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEE
ICANDPIEYQLPIRQTYLDAINDAKHYIYISNAYFLPDPLIRATLIDAAKRGVDIQIMVP
EECCCCCEEECCHHHHHHHHHCCCCEEEEEECEEECCCHHHHHHHHHHHHCCCEEEEEEC
EISDNIVVDWICRGLLGELMEHGARVLLYRGTMIHAKTMTVDGLWSTVGSANLDTRSLAA
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEEEEEEEECHHHHHCCCCCCCHHHEEE
NYEINATIKHPAFARQMEAMFLNDRAQSREINYQTWRSRSIFIHLGEQVLQPARGLF
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]