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The map label for this gene is pleD [H]

Identifier: 159900729

GI number: 159900729

Start: 5371329

End: 5373482

Strand: Reverse

Name: pleD [H]

Synonym: Haur_4216

Alternate gene names: 159900729

Gene position: 5373482-5371329 (Counterclockwise)

Preceding gene: 159900731

Following gene: 159900728

Centisome position: 84.67

GC content: 47.03

Gene sequence:

>2154_bases
ATGACCAAGCGACGATCCGTGCTACGGATCTCCTCGCGGCAACGAACTCACTTTCAAGCCAATCCTTGGCGAATTCGGCG
CATGATCACTGAGTTGCATGCGCTACACGAGATTCGCCACGCGATTAACCAGGCCTTGGAATTGGATACGGTGCTTGAGG
AGATTTATAGTCAAACATCGCGATTAATGGATACCCGCCAATTTTACTTGGCGTTATACTCGCCTGAACAAGAATTGATC
GAGTTTGCCTTGGCAGTTGAAAATGGCCAGCGCGTCTCTTGGCCCGCCCGCCCCTATGCCAATGGTTTAACCGAATGGAT
TTTGCAACATCGCCGCCCCCTGCGGATCAACGCCCATAACCTTGATCCTGCGGTTGCACCGATTATTGTTGGGCAGCCTG
TCCAATCATTTTTAGGGGTTCCAATTGTGCTTGGTGAGCAGGTTCTTGGGGTTATGAGTGTGCAATCGGTCGAACGAGCC
GAGGCCTATTCTGCCCACGATGAACAAATTTTGACCATGATCGCCGATCAGGCCGCTGTCGCGATTGCCCATGCCAAGCG
CTTTGCCGCCGTTGATCAACAGTTGCAAGCCCGCGTTGCTCAACTTGAAACCCTCGAAGCAACCATCCGCGATTTAAATG
AAACCCTCGATGTTGATACAATTCTGAATCGCTTACTTGACCGTATCCAGCCGATTATGCATGCTGATGCTGGGATGGTT
TGTTTGCTTGATACCGAACAGCAACGGATGTTTATCCGCGCAATTCAGGGCTATCCAGCTGAAGTTCGGCGTTTTCAATT
TGAAGGTTGGCCAATTGATCGTGGCATTGCGGGCTTAGTTGCACGTACCGGTCAACCTGATTGGACTGCTGATATTCGTA
ATTCGAGTTACTATGTTAATTCGCGGCCAACTACATTGAGCCAAATGACTGTGCCAATTGTGCATAGTGGCAAAGTTCTA
GGTGTAATGATTCTCGAAAGTGATCGAGTTGGCACATTTAATGATGATACGACGCGCTTTGTCAGCCAAATTGCTGATCA
TGCGGCGCTTTCAATTCATAATGCGCGTATTCATCAGCAAGCAGTTGAACAACAAGCGTTGTTGGCTCAACGCTCACAAC
AACTCAACGAAGTGCTGCGTATCAGCCAAGCATTAAGCGCCAACCTTAATTTAAATGATCTGCTGCCAGAAATTGTTCGA
GCAATTCAAGCAAGTTTGGGTTTCAATATTGCCTTGCTGAGTTTAGTTGATCAAGAACGACCAACCTTTATGCGTCGCCG
TGCCGTGGTTGGTGTGCCTGATGAACGCTGGTTTGAATTACGTGATCAATTAGTGCCAATTGAATGGTATCGCAGCGTAA
TGCGCGAAGAATTTCGGATTAGCCGCTCGTATTATATTCCGCATAGCCATACCAGCTATACCGCCATTTGGGGCAATAAC
AGTGATACGTATCGACCTGATTTAGGTGAGCGTCGTCCACATGAATGGCATCAAGATGATGCCTTGTTCGTGCCATTGTA
CGATTCTGATAATAATTTAATTAGTATTCTCTCAGTTGATGATCCACGTGATCGGCGGAAACCATCGTTTGAATCGGTGC
AGGTACTAGAAATTTTTGCCACTCAAGCAGCAATCGCGATTGAAAACGCCCATTTATATACAATTACCCAACAATTAGCC
ATCACCGATGGCTTAACTGGCTTGTTCAATCAACGCCATTTTATGACGATGCTTGATCGTGAAGTGGCCTTGGCCTATCG
CTATAATTATCCATTATCGTTATTAGCTTTGGATATTGATTATTTCAAGCAATATAATGATAACTATGGCCACTTGGTTG
GCAATGTGCTGCTGCGCGATTTTGCCCGGCTCATTTGCGAAAATGTACGCGATGTTGATATTGTTTCGCGCAACGGTGGC
GAAGAATTTACAATCATTTTGCCCAAAACTGATCAAGCAGGTGCAGTGTTGTTAGCCGAACGGCTGCGAGTTCGCACTGC
CGAACATCTTTTTGGTCAAGGCCATATTACGGTTAGCGTCGGCGTTGCCACCCTCAACAACAATTGGGATGCCCACACGT
TGCACGATCAAGCTGATCAAGCGCTTTATCGCGCCAAAAATTCTGGGCGTAATCTGGTTATCAGCGTCCCATAA

Upstream 100 bases:

>100_bases
GTATGCCCGCTGCTGGAATAGCATAGACAAGCCGAAAATCAACCTGTTATAATAGGCCATAACGGTGTTTACTAATCGCA
CAAAACGACCTGGACGCGAC

Downstream 100 bases:

>100_bases
TCGTCAGGTTTTTTGCGGTCGTGCTAGAATGGCAAGAGCAACACAACCAAGGAGTGTACTATGCCATCAACTTTGCCGAT
TATTGGGATACCGTGCGCAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 717; Mature: 716

Protein sequence:

>717_residues
MTKRRSVLRISSRQRTHFQANPWRIRRMITELHALHEIRHAINQALELDTVLEEIYSQTSRLMDTRQFYLALYSPEQELI
EFALAVENGQRVSWPARPYANGLTEWILQHRRPLRINAHNLDPAVAPIIVGQPVQSFLGVPIVLGEQVLGVMSVQSVERA
EAYSAHDEQILTMIADQAAVAIAHAKRFAAVDQQLQARVAQLETLEATIRDLNETLDVDTILNRLLDRIQPIMHADAGMV
CLLDTEQQRMFIRAIQGYPAEVRRFQFEGWPIDRGIAGLVARTGQPDWTADIRNSSYYVNSRPTTLSQMTVPIVHSGKVL
GVMILESDRVGTFNDDTTRFVSQIADHAALSIHNARIHQQAVEQQALLAQRSQQLNEVLRISQALSANLNLNDLLPEIVR
AIQASLGFNIALLSLVDQERPTFMRRRAVVGVPDERWFELRDQLVPIEWYRSVMREEFRISRSYYIPHSHTSYTAIWGNN
SDTYRPDLGERRPHEWHQDDALFVPLYDSDNNLISILSVDDPRDRRKPSFESVQVLEIFATQAAIAIENAHLYTITQQLA
ITDGLTGLFNQRHFMTMLDREVALAYRYNYPLSLLALDIDYFKQYNDNYGHLVGNVLLRDFARLICENVRDVDIVSRNGG
EEFTIILPKTDQAGAVLLAERLRVRTAEHLFGQGHITVSVGVATLNNNWDAHTLHDQADQALYRAKNSGRNLVISVP

Sequences:

>Translated_717_residues
MTKRRSVLRISSRQRTHFQANPWRIRRMITELHALHEIRHAINQALELDTVLEEIYSQTSRLMDTRQFYLALYSPEQELI
EFALAVENGQRVSWPARPYANGLTEWILQHRRPLRINAHNLDPAVAPIIVGQPVQSFLGVPIVLGEQVLGVMSVQSVERA
EAYSAHDEQILTMIADQAAVAIAHAKRFAAVDQQLQARVAQLETLEATIRDLNETLDVDTILNRLLDRIQPIMHADAGMV
CLLDTEQQRMFIRAIQGYPAEVRRFQFEGWPIDRGIAGLVARTGQPDWTADIRNSSYYVNSRPTTLSQMTVPIVHSGKVL
GVMILESDRVGTFNDDTTRFVSQIADHAALSIHNARIHQQAVEQQALLAQRSQQLNEVLRISQALSANLNLNDLLPEIVR
AIQASLGFNIALLSLVDQERPTFMRRRAVVGVPDERWFELRDQLVPIEWYRSVMREEFRISRSYYIPHSHTSYTAIWGNN
SDTYRPDLGERRPHEWHQDDALFVPLYDSDNNLISILSVDDPRDRRKPSFESVQVLEIFATQAAIAIENAHLYTITQQLA
ITDGLTGLFNQRHFMTMLDREVALAYRYNYPLSLLALDIDYFKQYNDNYGHLVGNVLLRDFARLICENVRDVDIVSRNGG
EEFTIILPKTDQAGAVLLAERLRVRTAEHLFGQGHITVSVGVATLNNNWDAHTLHDQADQALYRAKNSGRNLVISVP
>Mature_716_residues
TKRRSVLRISSRQRTHFQANPWRIRRMITELHALHEIRHAINQALELDTVLEEIYSQTSRLMDTRQFYLALYSPEQELIE
FALAVENGQRVSWPARPYANGLTEWILQHRRPLRINAHNLDPAVAPIIVGQPVQSFLGVPIVLGEQVLGVMSVQSVERAE
AYSAHDEQILTMIADQAAVAIAHAKRFAAVDQQLQARVAQLETLEATIRDLNETLDVDTILNRLLDRIQPIMHADAGMVC
LLDTEQQRMFIRAIQGYPAEVRRFQFEGWPIDRGIAGLVARTGQPDWTADIRNSSYYVNSRPTTLSQMTVPIVHSGKVLG
VMILESDRVGTFNDDTTRFVSQIADHAALSIHNARIHQQAVEQQALLAQRSQQLNEVLRISQALSANLNLNDLLPEIVRA
IQASLGFNIALLSLVDQERPTFMRRRAVVGVPDERWFELRDQLVPIEWYRSVMREEFRISRSYYIPHSHTSYTAIWGNNS
DTYRPDLGERRPHEWHQDDALFVPLYDSDNNLISILSVDDPRDRRKPSFESVQVLEIFATQAAIAIENAHLYTITQQLAI
TDGLTGLFNQRHFMTMLDREVALAYRYNYPLSLLALDIDYFKQYNDNYGHLVGNVLLRDFARLICENVRDVDIVSRNGGE
EFTIILPKTDQAGAVLLAERLRVRTAEHLFGQGHITVSVGVATLNNNWDAHTLHDQADQALYRAKNSGRNLVISVP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=39.1025641025641, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=37.8947368421053, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=36.6459627329193, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787802, Length=129, Percent_Identity=39.5348837209302, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=176, Percent_Identity=32.9545454545455, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=34.8314606741573, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=33.1210191082803, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=156, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=30.3225806451613, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 81740; Mature: 81609

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRRSVLRISSRQRTHFQANPWRIRRMITELHALHEIRHAINQALELDTVLEEIYSQTS
CCCCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMDTRQFYLALYSPEQELIEFALAVENGQRVSWPARPYANGLTEWILQHRRPLRINAHN
HHHHHHEEEEEEECCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCEEECCC
LDPAVAPIIVGQPVQSFLGVPIVLGEQVLGVMSVQSVERAEAYSAHDEQILTMIADQAAV
CCCHHHCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AIAHAKRFAAVDQQLQARVAQLETLEATIRDLNETLDVDTILNRLLDRIQPIMHADAGMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
CLLDTEQQRMFIRAIQGYPAEVRRFQFEGWPIDRGIAGLVARTGQPDWTADIRNSSYYVN
EEEECHHHHHHHHHHCCCCHHHHEEEECCCCCCCCHHHHHHCCCCCCCCEECCCCEEEEC
SRPTTLSQMTVPIVHSGKVLGVMILESDRVGTFNDDTTRFVSQIADHAALSIHNARIHQQ
CCCCCHHHEEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVEQQALLAQRSQQLNEVLRISQALSANLNLNDLLPEIVRAIQASLGFNIALLSLVDQER
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCC
PTFMRRRAVVGVPDERWFELRDQLVPIEWYRSVMREEFRISRSYYIPHSHTSYTAIWGNN
HHHHHHCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCC
SDTYRPDLGERRPHEWHQDDALFVPLYDSDNNLISILSVDDPRDRRKPSFESVQVLEIFA
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHCCCCCCCHHHHHHHHH
TQAAIAIENAHLYTITQQLAITDGLTGLFNQRHFMTMLDREVALAYRYNYPLSLLALDID
HHHHEEEECCEEEEEHHHHHHHCCHHHHHCCHHHHHHHHHHEEEEEEECCCEEEEEEEHH
YFKQYNDNYGHLVGNVLLRDFARLICENVRDVDIVSRNGGEEFTIILPKTDQAGAVLLAE
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHH
RLRVRTAEHLFGQGHITVSVGVATLNNNWDAHTLHDQADQALYRAKNSGRNLVISVP
HHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
TKRRSVLRISSRQRTHFQANPWRIRRMITELHALHEIRHAINQALELDTVLEEIYSQTS
CCCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMDTRQFYLALYSPEQELIEFALAVENGQRVSWPARPYANGLTEWILQHRRPLRINAHN
HHHHHHEEEEEEECCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCEEECCC
LDPAVAPIIVGQPVQSFLGVPIVLGEQVLGVMSVQSVERAEAYSAHDEQILTMIADQAAV
CCCHHHCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AIAHAKRFAAVDQQLQARVAQLETLEATIRDLNETLDVDTILNRLLDRIQPIMHADAGMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
CLLDTEQQRMFIRAIQGYPAEVRRFQFEGWPIDRGIAGLVARTGQPDWTADIRNSSYYVN
EEEECHHHHHHHHHHCCCCHHHHEEEECCCCCCCCHHHHHHCCCCCCCCEECCCCEEEEC
SRPTTLSQMTVPIVHSGKVLGVMILESDRVGTFNDDTTRFVSQIADHAALSIHNARIHQQ
CCCCCHHHEEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVEQQALLAQRSQQLNEVLRISQALSANLNLNDLLPEIVRAIQASLGFNIALLSLVDQER
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCC
PTFMRRRAVVGVPDERWFELRDQLVPIEWYRSVMREEFRISRSYYIPHSHTSYTAIWGNN
HHHHHHCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCC
SDTYRPDLGERRPHEWHQDDALFVPLYDSDNNLISILSVDDPRDRRKPSFESVQVLEIFA
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHCCCCCCCHHHHHHHHH
TQAAIAIENAHLYTITQQLAITDGLTGLFNQRHFMTMLDREVALAYRYNYPLSLLALDID
HHHHEEEECCEEEEEHHHHHHHCCHHHHHCCHHHHHHHHHHEEEEEEECCCEEEEEEEHH
YFKQYNDNYGHLVGNVLLRDFARLICENVRDVDIVSRNGGEEFTIILPKTDQAGAVLLAE
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHH
RLRVRTAEHLFGQGHITVSVGVATLNNNWDAHTLHDQADQALYRAKNSGRNLVISVP
HHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA