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The map label for this gene is rcnA [H]

Identifier: 159900671

GI number: 159900671

Start: 5304650

End: 5305993

Strand: Direct

Name: rcnA [H]

Synonym: Haur_4158

Alternate gene names: 159900671

Gene position: 5304650-5305993 (Clockwise)

Preceding gene: 159900669

Following gene: 159900672

Centisome position: 83.58

GC content: 53.05

Gene sequence:

>1344_bases
ATGAAACGTTGTTTATGCGCATGCTGGCTGCTGTTACTATTTGCCAGCCAACCACAAACCGCTCAGGCACACAGCCTTGA
TCAGCTTTTTCAAGATATGCGGGTGCAGATTCAGCCAACTCAAATTGTGCTGACCGTACACTTAATCGCTGGCCCGCTGA
TTACGCCGCGTTTGTGGGAGCACTTGGATACTGATCGTTCGCAAACGCTGGATCAAATTGAAATTGAGCGTTGGTGTGCT
GAGTTTAATCGCAATTTGGAATTGGCGTTTGATCACCAAGCACTCTCATTGACCTTGTATGAAGTCAGCGAATTTCCGAC
CACCAAAGCTGATTTTATTGGCTCGAATGCGACGAATTTAACCTGGACGGTGCAGGCAGCGCTTGGGCCAATCGCCACAG
GCGAACATCTGCTCAGCTTAAAATCGAATAATTACCATGATATTAGTGTTGTCGATTGGAGCAAAACCCGTGGCCGAGCA
GGGATTGTGGCCCAAGATGCTAGCACCATGGACTTGCATAGTGCTCAGTTTCCAATTAAATGGCCTAGCAATTTTCGCAG
CGATGGCGCGATTGCCTTGCCAACGGCGGAACCGAGTGCCCCGCAACAACCAAGCTCAAGCCTCGTGGCCCGTTTGCAAA
CTGGCGATAGTTCGTTTGGCTTGATCGCGGCAACGCTGGGGCTGGCTTTTGGATTTGGCGCGGTTCATGCCTTACAACCT
GGCCATGGCAAAACCTTGGTCGCGGCCTATCTGGTTGGCTCACGCGGCACGATTCAACAAGCGACCTTGCTGGGCGCAAT
TGTTACGCTGACCCATACTGCCAGTGTTTTTGCCTTGGGCGGGTTGATGTTGCTGTTCAGTGCTTGGGCTACGCCTGAGC
GCTTTATCCCAATTCTGACATTCGTTTCGGGAGTTTTGGTAGCAGGCTTGGGTTTGCGCATGCTCTGGGAGCGGCTGCAA
GCGCTGCGTTCTGGCAACCAAGGCCATTCCCATGGTGGCTTGTTTCATAGCCATGGCGATGGTGGGGCAGGCCATAGCCA
TGTGAGCAACCGCAAATTACTAGGCTTGGGCATTTCGGGTGGGCTTGTGCCCTGCCCCGAAGCCTTGGTGATTATGATTG
TTGCGGCGACGCTTGGCCGAATTGGCCTCGGCTTGGCGATGATTGTGGCGTTTAGTGCTGGTTTGGCGGCGGTCTTAATC
GGCATTGGGATTGTGCTGGTAACACTTGGCTCGCGCCTGATCAAAACCAGCCAGCCCGATGCCCGTTGGTTGCGTTGGCT
GCCAATCGCCAGCGCTGGTGTGGTGACAATCCTTGGAATTGGGCTTATGCTTCAAACAATCTAA

Upstream 100 bases:

>100_bases
AACGCGATAGCGTGCCATATGTGCTCCTTTATCGTAAAAACTACCGATTTGTAGGCAAGCGCTATGCCAGCGTTTAGGAT
TAATTTGTTATCATGCGGCT

Downstream 100 bases:

>100_bases
ATTTCTAGCAATCAGGAGCAGCGCATGGCATTGATTGTGGTCGTTGGGGGAGCTGATACTGGACGTGCTCCGATGGGTGC
GGTGCTATTACGCCGCTTGT

Product: high-affinity nickel-transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKRCLCACWLLLLFASQPQTAQAHSLDQLFQDMRVQIQPTQIVLTVHLIAGPLITPRLWEHLDTDRSQTLDQIEIERWCA
EFNRNLELAFDHQALSLTLYEVSEFPTTKADFIGSNATNLTWTVQAALGPIATGEHLLSLKSNNYHDISVVDWSKTRGRA
GIVAQDASTMDLHSAQFPIKWPSNFRSDGAIALPTAEPSAPQQPSSSLVARLQTGDSSFGLIAATLGLAFGFGAVHALQP
GHGKTLVAAYLVGSRGTIQQATLLGAIVTLTHTASVFALGGLMLLFSAWATPERFIPILTFVSGVLVAGLGLRMLWERLQ
ALRSGNQGHSHGGLFHSHGDGGAGHSHVSNRKLLGLGISGGLVPCPEALVIMIVAATLGRIGLGLAMIVAFSAGLAAVLI
GIGIVLVTLGSRLIKTSQPDARWLRWLPIASAGVVTILGIGLMLQTI

Sequences:

>Translated_447_residues
MKRCLCACWLLLLFASQPQTAQAHSLDQLFQDMRVQIQPTQIVLTVHLIAGPLITPRLWEHLDTDRSQTLDQIEIERWCA
EFNRNLELAFDHQALSLTLYEVSEFPTTKADFIGSNATNLTWTVQAALGPIATGEHLLSLKSNNYHDISVVDWSKTRGRA
GIVAQDASTMDLHSAQFPIKWPSNFRSDGAIALPTAEPSAPQQPSSSLVARLQTGDSSFGLIAATLGLAFGFGAVHALQP
GHGKTLVAAYLVGSRGTIQQATLLGAIVTLTHTASVFALGGLMLLFSAWATPERFIPILTFVSGVLVAGLGLRMLWERLQ
ALRSGNQGHSHGGLFHSHGDGGAGHSHVSNRKLLGLGISGGLVPCPEALVIMIVAATLGRIGLGLAMIVAFSAGLAAVLI
GIGIVLVTLGSRLIKTSQPDARWLRWLPIASAGVVTILGIGLMLQTI
>Mature_447_residues
MKRCLCACWLLLLFASQPQTAQAHSLDQLFQDMRVQIQPTQIVLTVHLIAGPLITPRLWEHLDTDRSQTLDQIEIERWCA
EFNRNLELAFDHQALSLTLYEVSEFPTTKADFIGSNATNLTWTVQAALGPIATGEHLLSLKSNNYHDISVVDWSKTRGRA
GIVAQDASTMDLHSAQFPIKWPSNFRSDGAIALPTAEPSAPQQPSSSLVARLQTGDSSFGLIAATLGLAFGFGAVHALQP
GHGKTLVAAYLVGSRGTIQQATLLGAIVTLTHTASVFALGGLMLLFSAWATPERFIPILTFVSGVLVAGLGLRMLWERLQ
ALRSGNQGHSHGGLFHSHGDGGAGHSHVSNRKLLGLGISGGLVPCPEALVIMIVAATLGRIGLGLAMIVAFSAGLAAVLI
GIGIVLVTLGSRLIKTSQPDARWLRWLPIASAGVVTILGIGLMLQTI

Specific function: Efflux system for nickel and cobalt [H]

COG id: COG2215

COG function: function code R; ABC-type uncharacterized transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788423, Length=249, Percent_Identity=28.1124497991968, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011541 [H]

Pfam domain/function: PF03824 NicO [H]

EC number: NA

Molecular weight: Translated: 47632; Mature: 47632

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRCLCACWLLLLFASQPQTAQAHSLDQLFQDMRVQIQPTQIVLTVHLIAGPLITPRLWE
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEECCCEEEEEEEEHHCCCCCHHHHH
HLDTDRSQTLDQIEIERWCAEFNRNLELAFDHQALSLTLYEVSEFPTTKADFIGSNATNL
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCE
TWTVQAALGPIATGEHLLSLKSNNYHDISVVDWSKTRGRAGIVAQDASTMDLHSAQFPIK
EEEEEEHHCCCCCCCHHEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCC
WPSNFRSDGAIALPTAEPSAPQQPSSSLVARLQTGDSSFGLIAATLGLAFGFGAVHALQP
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHGKTLVAAYLVGSRGTIQQATLLGAIVTLTHTASVFALGGLMLLFSAWATPERFIPILT
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FVSGVLVAGLGLRMLWERLQALRSGNQGHSHGGLFHSHGDGGAGHSHVSNRKLLGLGISG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCC
GLVPCPEALVIMIVAATLGRIGLGLAMIVAFSAGLAAVLIGIGIVLVTLGSRLIKTSQPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ARWLRWLPIASAGVVTILGIGLMLQTI
HHHHEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRCLCACWLLLLFASQPQTAQAHSLDQLFQDMRVQIQPTQIVLTVHLIAGPLITPRLWE
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEECCCEEEEEEEEHHCCCCCHHHHH
HLDTDRSQTLDQIEIERWCAEFNRNLELAFDHQALSLTLYEVSEFPTTKADFIGSNATNL
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCE
TWTVQAALGPIATGEHLLSLKSNNYHDISVVDWSKTRGRAGIVAQDASTMDLHSAQFPIK
EEEEEEHHCCCCCCCHHEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCC
WPSNFRSDGAIALPTAEPSAPQQPSSSLVARLQTGDSSFGLIAATLGLAFGFGAVHALQP
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHGKTLVAAYLVGSRGTIQQATLLGAIVTLTHTASVFALGGLMLLFSAWATPERFIPILT
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FVSGVLVAGLGLRMLWERLQALRSGNQGHSHGGLFHSHGDGGAGHSHVSNRKLLGLGISG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCC
GLVPCPEALVIMIVAATLGRIGLGLAMIVAFSAGLAAVLIGIGIVLVTLGSRLIKTSQPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ARWLRWLPIASAGVVTILGIGLMLQTI
HHHHEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA