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The map label for this gene is citA [H]

Identifier: 159900659

GI number: 159900659

Start: 5294260

End: 5295387

Strand: Direct

Name: citA [H]

Synonym: Haur_4146

Alternate gene names: 159900659

Gene position: 5294260-5295387 (Clockwise)

Preceding gene: 159900638

Following gene: 159900664

Centisome position: 83.42

GC content: 55.23

Gene sequence:

>1128_bases
ATGGCGACTGAAACCCAAGTTCATGTAGGTTTAGAAGGGATCGTTGCAGCGGCAACGCGGCTCAGCAGTGTCGATGGCCA
AGCTGGGGAATTAATTATTGCAGGCTTTCCCTTGGAGCGTTTGGCCCCGTTTGCGACCTTCGAGGAAACGATTTTTCTCT
TGTGGAATGACCATTTGCCAAGCCAAAGCGAGTTGGCCGAATTGCGCCAGAGCCTTGCCAGCCAGCGCCAATTGCCAGCC
CTTACCTTAGAAGTCGCTCAACAGCTGGGCCGCGAACAGGCCGACCCGATGGATGCATTGCGGGCTGCGACCGCTACATT
AAACCCATTGGCTGACGAAAAAGCTACGGCCCAACGAATTGTGGCAGCCTTGCCCACGATTGTGGCGGCCTATTGGCGAG
CACGCAACCAAGCCGAATTTATCGAGCCACGCAGCGATTTAAGCCATGCTGCCAATTATTTGTGGATGTTGACTGGCAAA
GAGCCAAGCGCTGAGAAGGTGCGGGCACTCGAAACCTATCTCAACACCGTAGTTGACCATGGCCTGAATGCCTCGACCTT
CACTACTCGCGTGATCATCTCGACTGAATCGGATTTGGTTTCGGCGATTACTGGGGCGATTGGAGCGCTCAAAGGGCCGT
TGCATGGCGGCGCACCTGGCCCAGCCTTGGATATGGTATTTGAAATTGGCACAGCCGATCGCGCTGAGGAAGTACTTCGC
GCCAAGTTAGCACGCGGCGAGCGCTTGATGGGCTTTGGCCATCGCGTCTACAAGGTGCGCGATCCACGGGCCGAGGTTTT
GGCAGGCGCAGCCGATCAACTTTTTGCCAACGATGGCAACCGCGAGTTATACGAACTTGTACGCCATGTTGAGCAAACCG
CGATTCGGCTGCTCGAAGAACACAAGCCAGGCCGCAAATTGCAAACCAATGTCGAGTTCTACACTGCCTTGCTGTTGCAT
GGCATCGATTTTGAAACCGACCTGTTTACCCCAACCTTTACGATCAGCCGCGCTGTTGGTTGGATTGCCCACGCCTTCGA
GCAACGCGCCGTTGGCCGAATTATTCGCCCACAATCGATTTATACTGGCGAACGCAACCGCACGTGGGTTGAGGTTGCCG
AGCGGTAA

Upstream 100 bases:

>100_bases
TGACAACATTGATAAATCTATTTATAGTCGATAGCAGCAGGAATTTCAAGCGGCAAAATTGCCGCTCAAGGCCTAACACG
CTGGAAAGGATCGATTGTCA

Downstream 100 bases:

>100_bases
AATATCCCATCACCCTCGCCCCCTCGCCCACTGCGGCGAACGAGAGGTGCAATTTCAGAATGGTTCCCCTCGTCTGCTGG
GCGGGCGAGGGGGTGAGGGA

Product: citrate (Si)-synthase

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MATETQVHVGLEGIVAAATRLSSVDGQAGELIIAGFPLERLAPFATFEETIFLLWNDHLPSQSELAELRQSLASQRQLPA
LTLEVAQQLGREQADPMDALRAATATLNPLADEKATAQRIVAALPTIVAAYWRARNQAEFIEPRSDLSHAANYLWMLTGK
EPSAEKVRALETYLNTVVDHGLNASTFTTRVIISTESDLVSAITGAIGALKGPLHGGAPGPALDMVFEIGTADRAEEVLR
AKLARGERLMGFGHRVYKVRDPRAEVLAGAADQLFANDGNRELYELVRHVEQTAIRLLEEHKPGRKLQTNVEFYTALLLH
GIDFETDLFTPTFTISRAVGWIAHAFEQRAVGRIIRPQSIYTGERNRTWVEVAER

Sequences:

>Translated_375_residues
MATETQVHVGLEGIVAAATRLSSVDGQAGELIIAGFPLERLAPFATFEETIFLLWNDHLPSQSELAELRQSLASQRQLPA
LTLEVAQQLGREQADPMDALRAATATLNPLADEKATAQRIVAALPTIVAAYWRARNQAEFIEPRSDLSHAANYLWMLTGK
EPSAEKVRALETYLNTVVDHGLNASTFTTRVIISTESDLVSAITGAIGALKGPLHGGAPGPALDMVFEIGTADRAEEVLR
AKLARGERLMGFGHRVYKVRDPRAEVLAGAADQLFANDGNRELYELVRHVEQTAIRLLEEHKPGRKLQTNVEFYTALLLH
GIDFETDLFTPTFTISRAVGWIAHAFEQRAVGRIIRPQSIYTGERNRTWVEVAER
>Mature_374_residues
ATETQVHVGLEGIVAAATRLSSVDGQAGELIIAGFPLERLAPFATFEETIFLLWNDHLPSQSELAELRQSLASQRQLPAL
TLEVAQQLGREQADPMDALRAATATLNPLADEKATAQRIVAALPTIVAAYWRARNQAEFIEPRSDLSHAANYLWMLTGKE
PSAEKVRALETYLNTVVDHGLNASTFTTRVIISTESDLVSAITGAIGALKGPLHGGAPGPALDMVFEIGTADRAEEVLRA
KLARGERLMGFGHRVYKVRDPRAEVLAGAADQLFANDGNRELYELVRHVEQTAIRLLEEHKPGRKLQTNVEFYTALLLHG
IDFETDLFTPTFTISRAVGWIAHAFEQRAVGRIIRPQSIYTGERNRTWVEVAER

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=392, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1786527, Length=377, Percent_Identity=34.2175066312997, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786939, Length=386, Percent_Identity=30.3108808290155, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=344, Percent_Identity=27.3255813953488, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319850, Length=410, Percent_Identity=26.3414634146341, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=343, Percent_Identity=25.0728862973761, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325257, Length=361, Percent_Identity=25.4847645429363, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640126, Length=397, Percent_Identity=26.1964735516373, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640124, Length=400, Percent_Identity=26, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356863, Length=350, Percent_Identity=24.5714285714286, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41277; Mature: 41146

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATETQVHVGLEGIVAAATRLSSVDGQAGELIIAGFPLERLAPFATFEETIFLLWNDHLP
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCHHHHHHEEEEEECCCCC
SQSELAELRQSLASQRQLPALTLEVAQQLGREQADPMDALRAATATLNPLADEKATAQRI
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VAALPTIVAAYWRARNQAEFIEPRSDLSHAANYLWMLTGKEPSAEKVRALETYLNTVVDH
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GLNASTFTTRVIISTESDLVSAITGAIGALKGPLHGGAPGPALDMVFEIGTADRAEEVLR
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
AKLARGERLMGFGHRVYKVRDPRAEVLAGAADQLFANDGNRELYELVRHVEQTAIRLLEE
HHHHCCCHHHHCCCEEEEECCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
HKPGRKLQTNVEFYTALLLHGIDFETDLFTPTFTISRAVGWIAHAFEQRAVGRIIRPQSI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YTGERNRTWVEVAER
ECCCCCCCCEEECCC
>Mature Secondary Structure 
ATETQVHVGLEGIVAAATRLSSVDGQAGELIIAGFPLERLAPFATFEETIFLLWNDHLP
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCHHHHHHEEEEEECCCCC
SQSELAELRQSLASQRQLPALTLEVAQQLGREQADPMDALRAATATLNPLADEKATAQRI
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VAALPTIVAAYWRARNQAEFIEPRSDLSHAANYLWMLTGKEPSAEKVRALETYLNTVVDH
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GLNASTFTTRVIISTESDLVSAITGAIGALKGPLHGGAPGPALDMVFEIGTADRAEEVLR
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
AKLARGERLMGFGHRVYKVRDPRAEVLAGAADQLFANDGNRELYELVRHVEQTAIRLLEE
HHHHCCCHHHHCCCEEEEECCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
HKPGRKLQTNVEFYTALLLHGIDFETDLFTPTFTISRAVGWIAHAFEQRAVGRIIRPQSI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YTGERNRTWVEVAER
ECCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]