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The map label for this gene is pyrB [H]

Identifier: 159900505

GI number: 159900505

Start: 5092779

End: 5093735

Strand: Direct

Name: pyrB [H]

Synonym: Haur_3992

Alternate gene names: 159900505

Gene position: 5092779-5093735 (Clockwise)

Preceding gene: 159900504

Following gene: 159900508

Centisome position: 80.24

GC content: 54.23

Gene sequence:

>957_bases
ATGAGTTCGCCAACCACTACACCGCGCCGTCGGCATGTGCTTGATCTTGATGATTGGAGTGCTGATGAGTTACGCACACT
TTTGACCATCGCCACCCAGATGAAGGATGTGCTGGAGCGTCCGGTTAAGCAAGTGCCAACGTTGCGCGGCAAAATTGTGG
TCAATATGTTTTGGGAAGATAGCACCCGCACTCGTTCGTCATTTGAGCTAGCGGCCAAAGCGCTCTCGGCCAACGTGCTC
AGCCTCGGAGCCAAAGATACCAGCGTCAAAAAAGGCGAATCATTGGTCGATACGGTGCGCACGGTGCAAGCCTTGGGAGC
CGATTATATTGTGATGCGCCATGGCCAAAGTGGCTCGCCCTATCTGGTGGCGCAGCATTTCCGTGGCTCGGTGATTAATG
GTGGCGATGGTCGGCATGCCCATCCATCGCAAGCCCTGCTCGACCTATTCACGATGCACGAACGGCTAGGTGAGTTGGCC
GACAAAAAAGTCGTGATTGTTGGTGATATTTTGCATAGCCGCGTGGCTCGCTCCAATTTGTGGGCGTTGACGACGATGGG
CGCAAAAGTCGTGGTTTGTGGCCCACCAACCTTGATCGGCCCAGCCAAATATTGGCAAAGCACATGGCCCAATGTTGAAA
TTTCGCATAGCCTTGATCAAGCTGCTGAGGGTGCTGATGTGCTGATGGCCTTGCGTTTGCAACACGAACGCATGGAAGGT
GGTTTGTTGCCATCATTGCGCGAATATAGCCGCCGCTTTGGCCTGAATGCTACCCGCGTCGCCAGCGCCAACTCCAACGT
GATTGTGATGCACCCCGGCCCGATGAACGAAGGCGTGGAGATTATGCCCGATGTAGCAACTGGCCCACACTCAGTAATTG
AAACCCAAATTACCAATGGCGTAGCCATGCGCATGGCCTTGTTCTACTACCTTGCCACTGGTAGCGAACAGGTGTAG

Upstream 100 bases:

>100_bases
AGCAGCCTTGCCAGCCCGTCGCTAAACCGTCAAGGCGCTTAGGCCACAACCTAAGCGCTTTTGTTTATTAGTTGCTACTT
GCTATAGATGGAGAATTTTT

Downstream 100 bases:

>100_bases
ATCCGATCCATCATCAGGATTGTGGCCCCTCGCCCATACGAGTGAGCGAGGGGCTTTTTGCGCTGAACCAGAGGCGAAGG
CCACGAATTAACGTTGGACT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSSPTTTPRRRHVLDLDDWSADELRTLLTIATQMKDVLERPVKQVPTLRGKIVVNMFWEDSTRTRSSFELAAKALSANVL
SLGAKDTSVKKGESLVDTVRTVQALGADYIVMRHGQSGSPYLVAQHFRGSVINGGDGRHAHPSQALLDLFTMHERLGELA
DKKVVIVGDILHSRVARSNLWALTTMGAKVVVCGPPTLIGPAKYWQSTWPNVEISHSLDQAAEGADVLMALRLQHERMEG
GLLPSLREYSRRFGLNATRVASANSNVIVMHPGPMNEGVEIMPDVATGPHSVIETQITNGVAMRMALFYYLATGSEQV

Sequences:

>Translated_318_residues
MSSPTTTPRRRHVLDLDDWSADELRTLLTIATQMKDVLERPVKQVPTLRGKIVVNMFWEDSTRTRSSFELAAKALSANVL
SLGAKDTSVKKGESLVDTVRTVQALGADYIVMRHGQSGSPYLVAQHFRGSVINGGDGRHAHPSQALLDLFTMHERLGELA
DKKVVIVGDILHSRVARSNLWALTTMGAKVVVCGPPTLIGPAKYWQSTWPNVEISHSLDQAAEGADVLMALRLQHERMEG
GLLPSLREYSRRFGLNATRVASANSNVIVMHPGPMNEGVEIMPDVATGPHSVIETQITNGVAMRMALFYYLATGSEQV
>Mature_317_residues
SSPTTTPRRRHVLDLDDWSADELRTLLTIATQMKDVLERPVKQVPTLRGKIVVNMFWEDSTRTRSSFELAAKALSANVLS
LGAKDTSVKKGESLVDTVRTVQALGADYIVMRHGQSGSPYLVAQHFRGSVINGGDGRHAHPSQALLDLFTMHERLGELAD
KKVVIVGDILHSRVARSNLWALTTMGAKVVVCGPPTLIGPAKYWQSTWPNVEISHSLDQAAEGADVLMALRLQHERMEGG
LLPSLREYSRRFGLNATRVASANSNVIVMHPGPMNEGVEIMPDVATGPHSVIETQITNGVAMRMALFYYLATGSEQV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=35.5263157894737, Blast_Score=167, Evalue=8e-42,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=35.6913183279743, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786469, Length=172, Percent_Identity=34.8837209302326, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790703, Length=232, Percent_Identity=30.6034482758621, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=347, Percent_Identity=29.3948126801153, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=30.9446254071661, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=30.9904153354633, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34815; Mature: 34684

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTTTPRRRHVLDLDDWSADELRTLLTIATQMKDVLERPVKQVPTLRGKIVVNMFWED
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
STRTRSSFELAAKALSANVLSLGAKDTSVKKGESLVDTVRTVQALGADYIVMRHGQSGSP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
YLVAQHFRGSVINGGDGRHAHPSQALLDLFTMHERLGELADKKVVIVGDILHSRVARSNL
EEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCC
WALTTMGAKVVVCGPPTLIGPAKYWQSTWPNVEISHSLDQAAEGADVLMALRLQHERMEG
EEEEECCCEEEEECCCCCCCCHHHHHCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHCC
GLLPSLREYSRRFGLNATRVASANSNVIVMHPGPMNEGVEIMPDVATGPHSVIETQITNG
CCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHH
VAMRMALFYYLATGSEQV
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSPTTTPRRRHVLDLDDWSADELRTLLTIATQMKDVLERPVKQVPTLRGKIVVNMFWED
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
STRTRSSFELAAKALSANVLSLGAKDTSVKKGESLVDTVRTVQALGADYIVMRHGQSGSP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
YLVAQHFRGSVINGGDGRHAHPSQALLDLFTMHERLGELADKKVVIVGDILHSRVARSNL
EEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCC
WALTTMGAKVVVCGPPTLIGPAKYWQSTWPNVEISHSLDQAAEGADVLMALRLQHERMEG
EEEEECCCEEEEECCCCCCCCHHHHHCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHCC
GLLPSLREYSRRFGLNATRVASANSNVIVMHPGPMNEGVEIMPDVATGPHSVIETQITNG
CCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHH
VAMRMALFYYLATGSEQV
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA