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The map label for this gene is braE [H]

Identifier: 159900317

GI number: 159900317

Start: 4770873

End: 4771904

Strand: Reverse

Name: braE [H]

Synonym: Haur_3800

Alternate gene names: 159900317

Gene position: 4771904-4770873 (Counterclockwise)

Preceding gene: 159900318

Following gene: 159900316

Centisome position: 75.19

GC content: 53.59

Gene sequence:

>1032_bases
ATGCAGCGTTTGCGCCCACTACTGCCACTTGTGGCGATTGGCGGCCTTGCAAGCTTGCCATTTGCCTTTGCCGCCGATAC
CACCCGCTTTTGGCAAGGGGTTTTCATTCAGATTTTTATTCTGGCGATTTATGCCCTGAGCTACGATTTGCTGATGGGCT
ACACCGGCATGATTTCGTTTGGCCATGCGCTGTTTTTTGGCGCTGGTGGCTATACCGCCGCCATTTTGCTGCGCCAAGCC
GAGCCGCCCTCGATGCTAATTGTGATTGCAGCGGTGATTGTGGTTGGCGCAACTATCGGGGCAGCAGTGGGCGCATTGTC
GCTACGGGTCAGCGGGGTCTATTTTTCGATGATTACCCTCGCGCTGGCCGAAATTGCCTTCATCCTGTTCAAAGCCGATG
ATCCCAAGCTCAAGCCAATTACGGGCGGCGAAATTGGCTTGCAAGGGATTGTCGTGCCCGCAGCGATCGATGCCACGACC
TATCGACTGCGCTTTTACTTTTTGACCTTGGCGTGTATGGTTGGCTTGTATTACGCGGCGCGGCGTTTGATCAACTCGCC
AACAGGGCGGGTATTTGTGGCAATTCGCGAGAACGAGCCACGCGCCACGGCCTTGGGCTATAACACGCTGCATTTCAAAT
TATTGGCAACTGCAATTTCATCAACGATTGCGGCCTTGGCAGGGATGTTGATGGTGCTGTATGAAAAAAGTGCCAGCTTC
GAAATGCTCAGCGTTAATCTCACCATTCAAGCCTTGTTGATGACGATCATCGGCGGGATTGGCACGCTAATCGGGCCAAT
GCTCGGTGCTGCTACCATCCGCTTGCTTGATCATGGCCTCAAAGGCGAATTTATGCAGGCACTTTTGCCAGCGTGGTTGC
GCCCCGACTTAGTATTTGGCATCGTGTATGTGGTGCTGGTGTTATTCTTCCCGGCTGGTTTGATGGGAGCAATTCGCAAG
TTGCGCGGCAAAACCAGCCGTAGCTCAGTCGAACGCCTACGCCAAGCCATGAAACCAAAGGCTGAATCATGA

Upstream 100 bases:

>100_bases
GGTGCTGGCAAATTGTGGCCACTCGCCACAGATCGAACAGCCCAGTTTGTTCAATACGGCTCTACTCGATTTTCTGACCC
AAGTTTAGGCGAGGTTTGCT

Downstream 100 bases:

>100_bases
CCTACGAATTTCCGCCAATTGGCATTGCTGCAATCGCGACCTATCTGCCAGCAGCCGTCGAGACTGCTAGTCAAATCGCC
TTAGCCGCCAATATCCCCGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MQRLRPLLPLVAIGGLASLPFAFAADTTRFWQGVFIQIFILAIYALSYDLLMGYTGMISFGHALFFGAGGYTAAILLRQA
EPPSMLIVIAAVIVVGATIGAAVGALSLRVSGVYFSMITLALAEIAFILFKADDPKLKPITGGEIGLQGIVVPAAIDATT
YRLRFYFLTLACMVGLYYAARRLINSPTGRVFVAIRENEPRATALGYNTLHFKLLATAISSTIAALAGMLMVLYEKSASF
EMLSVNLTIQALLMTIIGGIGTLIGPMLGAATIRLLDHGLKGEFMQALLPAWLRPDLVFGIVYVVLVLFFPAGLMGAIRK
LRGKTSRSSVERLRQAMKPKAES

Sequences:

>Translated_343_residues
MQRLRPLLPLVAIGGLASLPFAFAADTTRFWQGVFIQIFILAIYALSYDLLMGYTGMISFGHALFFGAGGYTAAILLRQA
EPPSMLIVIAAVIVVGATIGAAVGALSLRVSGVYFSMITLALAEIAFILFKADDPKLKPITGGEIGLQGIVVPAAIDATT
YRLRFYFLTLACMVGLYYAARRLINSPTGRVFVAIRENEPRATALGYNTLHFKLLATAISSTIAALAGMLMVLYEKSASF
EMLSVNLTIQALLMTIIGGIGTLIGPMLGAATIRLLDHGLKGEFMQALLPAWLRPDLVFGIVYVVLVLFFPAGLMGAIRK
LRGKTSRSSVERLRQAMKPKAES
>Mature_343_residues
MQRLRPLLPLVAIGGLASLPFAFAADTTRFWQGVFIQIFILAIYALSYDLLMGYTGMISFGHALFFGAGGYTAAILLRQA
EPPSMLIVIAAVIVVGATIGAAVGALSLRVSGVYFSMITLALAEIAFILFKADDPKLKPITGGEIGLQGIVVPAAIDATT
YRLRFYFLTLACMVGLYYAARRLINSPTGRVFVAIRENEPRATALGYNTLHFKLLATAISSTIAALAGMLMVLYEKSASF
EMLSVNLTIQALLMTIIGGIGTLIGPMLGAATIRLLDHGLKGEFMQALLPAWLRPDLVFGIVYVVLVLFFPAGLMGAIRK
LRGKTSRSSVERLRQAMKPKAES

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=323, Percent_Identity=25.077399380805, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36873; Mature: 36873

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLRPLLPLVAIGGLASLPFAFAADTTRFWQGVFIQIFILAIYALSYDLLMGYTGMISF
CCCHHHHHHHHHHCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHALFFGAGGYTAAILLRQAEPPSMLIVIAAVIVVGATIGAAVGALSLRVSGVYFSMITL
HHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAEIAFILFKADDPKLKPITGGEIGLQGIVVPAAIDATTYRLRFYFLTLACMVGLYYAA
HHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
RRLINSPTGRVFVAIRENEPRATALGYNTLHFKLLATAISSTIAALAGMLMVLYEKSASF
HHHHCCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EMLSVNLTIQALLMTIIGGIGTLIGPMLGAATIRLLDHGLKGEFMQALLPAWLRPDLVFG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHH
IVYVVLVLFFPAGLMGAIRKLRGKTSRSSVERLRQAMKPKAES
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQRLRPLLPLVAIGGLASLPFAFAADTTRFWQGVFIQIFILAIYALSYDLLMGYTGMISF
CCCHHHHHHHHHHCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHALFFGAGGYTAAILLRQAEPPSMLIVIAAVIVVGATIGAAVGALSLRVSGVYFSMITL
HHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAEIAFILFKADDPKLKPITGGEIGLQGIVVPAAIDATTYRLRFYFLTLACMVGLYYAA
HHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
RRLINSPTGRVFVAIRENEPRATALGYNTLHFKLLATAISSTIAALAGMLMVLYEKSASF
HHHHCCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EMLSVNLTIQALLMTIIGGIGTLIGPMLGAATIRLLDHGLKGEFMQALLPAWLRPDLVFG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHH
IVYVVLVLFFPAGLMGAIRKLRGKTSRSSVERLRQAMKPKAES
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]